scholarly journals Effects of peptidic fractions from Saccharomyces cerevisiae culture on growth and metabolism of the ruminal bacteria Megasphaera elsdenii

2004 ◽  
Vol 53 (3) ◽  
pp. 177-186 ◽  
Author(s):  
Filippo Rossi ◽  
Aldo Di Luccia ◽  
Donatella Vincenti ◽  
Pier Sandro Cocconcelli
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ruminal Bacteria ◽  
Megasphaera Elsdenii

Effects of a strain of Saccharomyces cerevisiae (Levucell® SC), a microbial additive for ruminants, on lactate metabolism in vitro

1996 ◽  
Vol 42 (9) ◽  
pp. 927-933 ◽  
Author(s):  
Frédérique Chaucheyras ◽  
Gérard Fonty ◽  
Philippe Gouet ◽  
Gérard Bertin ◽  
Jean-Michel Salmon
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acids ◽  
Lactate Concentration ◽  
Lactate Production ◽  
Yeast Cells ◽  
Strictly Anaerobic ◽  
Streptococcus Bovis ◽  
Lactate Metabolism ◽  
Megasphaera Elsdenii

The effect of Levucell® SC, a strain of Saccharomyces cerevisiae marked as a feed additive for ruminants, was investigated in vitro on lactate metabolism by the ruminal bacteria Streptococcus bovis and Megasphaera elsdenii. The coculture between 107 live cells∙mL−1 of SC and a Streptococcus bovis strain in the presence of glucose reduced lactate production by the bacterial strain. Live yeast cells were able to compete with Streptococcus bovis for glucose utilization in strictly anaerobic conditions, so less glucose was available for the bacterium. SC also stimulated L-lactate utilization by a strain of M. elsdenii. The effect depended on the concentration of yeast cells added. Bacterial growth and fermentation end-product concentrations were also increased in the presence of SC. Some amino acids and vitamins, but not dicarboxylic acids, stimulated the bacterial specific activity of L-lactate uptake. SC was able to provide amino acids to M. elsdenii. In a coculture of Streptococcus bovis and M. elsdenii on glucose, the reduction of lactate concentration was improved by SC, the same trend being observed when maltose or soluble starch were used as carbon and energy source. These results indicate that SC can be a very useful tool to reduce lactate accumulation in vitro during fermentation of soluble sugars.Key words: rumen, Saccharomyces cerevisiae, lactate metabolism, Streptococcus bovis, Megasphaera elsdenii.

The effect of Saccharomyces cerevisiae on in vitro growth and fermentation of Selenomonas ruminantium and Megasphaera elsdenii

2017 ◽  
Vol 13 (3) ◽  
pp. 9-22
Author(s):  
Sylwia Grochowska ◽  
Włodzimierz Nowak ◽  
Małgorzata Lasik-Kurdyś ◽  
Robert Mikuła ◽  
Jacek Nowak
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Volatile Fatty Acids ◽  
Yeast Fermentation ◽  
Yeast Culture ◽  
In Vitro Growth ◽  
Fermentation Products ◽  
Selenomonas Ruminantium ◽  
Megasphaera Elsdenii ◽  
Live Culture

Stimulation of lactate utilization by Selenomonas ruminantium and Megasphaera elsdenii may help in reducing problems associated with rumen acidosis. The objective of this study was to determine the effect of a Saccharomyces cerevisiae live culture and Saccharomyces cerevisiae fermentation products on in vitro growth and fermentation of lactate-utilizing ruminal bacteria, S. ruminantium (ATCC 19205) and M. elsdenii (ATCC 25940). The cultures were run for 0, 6, 12, 24 and 48 h under anaerobic conditions on a growth medium supplemented with a yeast live culture (SC) or with yeast fermentation products (SCFP) and, as reference, on the same medium without supplementation (CON). Neither SC nor SCFP had a significant effect on the growth of S. ruminantium after 6, 12 and 24 h of incubation, but the live yeast culture significantly (P≤0.05) improved the growth of these bacteria after 48 h of incubation. The yeast fermentation products significantly (P≤0.05) decreased pH and increased lactate synthesis by S. ruminantium. The Saccharomyces cerevisiae live culture significantly improved the growth of M. elsdenii after 12 and 24 h of incubation, and the S. cerevisiae fermentation products increased its growth after 48 h. The After 24 and 48 h of incubation the Saccharomyces cerevisiae live culture reduced the concentration of total volatile fatty acids (VFA), while caproate was the main product of in vitro fermentation of M. elsdenii (P≤0.05). Saccharomyces cerevisiae live cultures may improve microbial fibre fermentation in the rumen by maintaining optimal pH conditions.

scholarly journals Effects of different dietary concentrate to forage ratio and thiamine supplementation on the rumen fermentation and ruminal bacterial community in dairy cows

2015 ◽  
Vol 55 (2) ◽  
pp. 189 ◽  
Author(s):  
Hongrong Wang ◽  
Xiaohua Pan ◽  
Chao Wang ◽  
Mengzhi Wang ◽  
Lihuai Yu
Keyword(s):  
Bacterial Community ◽  
Dairy Cows ◽  
Ph Value ◽  
Latin Square ◽  
Population Variation ◽  
Control Group ◽  
Ruminal Bacteria ◽  
Megasphaera Elsdenii ◽  
The Mean ◽  
The Difference

A subacute ruminal acidosis (SARA) model was induced gradually by increasing the proportion of dietary concentrate to evaluate the effect of thiamine supplementation on the structure of bacterial community in dairy cows. Three Holstein dairy cows with rumen cannula were randomly assigned to a replicated 3 × 3 Latin square design trial and received three diets during three successive 21-day periods in each square. The three dietary treatments were as follows: a low-concentrate diet (control), a high-concentrate SARA-induced diet (SARA) and a high-concentrate SARA-induced diet with 180 mg thiamine/kg DM (SARA+thiamine). Real-time–polymerase chain reaction assay was used to quantify the population variation of SARA-related ruminal bacteria in these cows. The results showed that SARA was induced gradually when cows were fed with the high-concentrate diets. The mean ruminal pH value was higher in the control cows than in those of SARA and SARA+thiamine groups, the mean was decreased in cows fed on SARA diet, and the depression was alleviated by supplemented thiamine and the difference was significant (P < 0.05) especially at 9-h and 12-h sample times (or 1 h and 4 h after the second feeding). The populations of Streptococcus bovis and genus Lactobacillus in cows from the SARA group were increased in log copies/µL by 3.62% and 4.65%, respectively, compared with the control group (P < 0.05). In contrast, in log copies/µL, populations of Butyrivibrio fibrisovens and Megasphaera elsdenii were decreased by 1.14% and 4.90%, respectively (P < 0.05). Thiamine supplementation led to an obvious reduction of Strepococcus bovis and Lactobacillus (P < 0.05), whereas the number of log copies/µL of Megasphaera elsdenii was dramatically increased (P < 0.05). There was no significant effect of thiamine supplementation on the number of log copies/µL of Butyrivibrio fibrisovens and Selenomonas ruminantium (P > 0.05). It was concluded that thiamine supplementation to high-concentrate diets at concentrations of 180 mg/kg DM could help alleviate SARA by increasing rumen pH and balancing the population of lactic acid-producing and -consuming bacteria.

Anti-Saccharomyces cerevisiae Antibodies in Inflammatory Bowel Disease: a Family Study

2001 ◽  
Vol 36 (2) ◽  
pp. 196-201 ◽  
Author(s):  
F. Seibold ◽  
O. Stich ◽  
R. Hufnagl ◽  
S. Kamil ◽  
M. Scheurlen
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Inflammatory Bowel Disease ◽  
Bowel Disease ◽  
Family Study ◽  
Inflammatory Bowel
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