scholarly journals Effects of live cells of Saccharomyces cerevisiae on in vitro lactate utilization by Megasphaera elsdenii and on the interactions between Streptococcus bovis and M. elsdenii grown on glucose

1996 ◽  
Vol 45 (Suppl. 1) ◽  
pp. 338-338
Author(s):  
F. Chaucheyras ◽  
G. Fonty ◽  
G. Bertin ◽  
Ph Gouet
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Live Cells ◽  
Streptococcus Bovis ◽  
Megasphaera Elsdenii ◽  
Lactate Utilization

Effects of a strain of Saccharomyces cerevisiae (Levucell® SC), a microbial additive for ruminants, on lactate metabolism in vitro

1996 ◽  
Vol 42 (9) ◽  
pp. 927-933 ◽  
Author(s):  
Frédérique Chaucheyras ◽  
Gérard Fonty ◽  
Philippe Gouet ◽  
Gérard Bertin ◽  
Jean-Michel Salmon
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Amino Acids ◽  
Lactate Concentration ◽  
Lactate Production ◽  
Yeast Cells ◽  
Strictly Anaerobic ◽  
Streptococcus Bovis ◽  
Lactate Metabolism ◽  
Megasphaera Elsdenii

The effect of Levucell® SC, a strain of Saccharomyces cerevisiae marked as a feed additive for ruminants, was investigated in vitro on lactate metabolism by the ruminal bacteria Streptococcus bovis and Megasphaera elsdenii. The coculture between 107 live cells∙mL−1 of SC and a Streptococcus bovis strain in the presence of glucose reduced lactate production by the bacterial strain. Live yeast cells were able to compete with Streptococcus bovis for glucose utilization in strictly anaerobic conditions, so less glucose was available for the bacterium. SC also stimulated L-lactate utilization by a strain of M. elsdenii. The effect depended on the concentration of yeast cells added. Bacterial growth and fermentation end-product concentrations were also increased in the presence of SC. Some amino acids and vitamins, but not dicarboxylic acids, stimulated the bacterial specific activity of L-lactate uptake. SC was able to provide amino acids to M. elsdenii. In a coculture of Streptococcus bovis and M. elsdenii on glucose, the reduction of lactate concentration was improved by SC, the same trend being observed when maltose or soluble starch were used as carbon and energy source. These results indicate that SC can be a very useful tool to reduce lactate accumulation in vitro during fermentation of soluble sugars.Key words: rumen, Saccharomyces cerevisiae, lactate metabolism, Streptococcus bovis, Megasphaera elsdenii.

Increase of ruminal fiber digestion by cellobiose and a twin strain of Saccharomyces cerevisiae live cells in vitro

2006 ◽  
Vol 77 (4) ◽  
pp. 407-413 ◽  
Author(s):  
Zeenat Ara LILA ◽  
Nazimuddin MOHAMMED ◽  
Tsuyoshi TAKAHASHI ◽  
Masahiko TABATA ◽  
Takashi YASUI ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Live Cells ◽  
Fiber Digestion

Potential influence of dairy propionibacteria on the growth and acid metabolism of Streptococcus bovis and Megasphaera elsdenii

2017 ◽  
Vol 8 (1) ◽  
pp. 111-119 ◽  
Author(s):  
J. Luo ◽  
C.S. Ranadheera ◽  
S. King ◽  
C.A. Evans ◽  
S.K. Baines
Keyword(s):  
Lactic Acid ◽  
Propionic Acid ◽  
Acid Metabolism ◽  
Feedlot Cattle ◽  
Streptococcus Bovis ◽  
In Vitro Investigation ◽  
Megasphaera Elsdenii ◽  
Dairy Propionibacteria ◽  
Different Strains

Ruminal acidosis is a prevalent disorder among dairy cows and feedlot cattle, which can significantly impair their health and productivity. This study, involving seven different strains of dairy propionibacteria, represents an in vitro investigation of the feasibility of using these organisms as direct-fed microbials to control lactic acid acumulation in the rumen. Interactions between the propionibacteria, Streptococcus bovis and Megasphaera elsdenii were evaluated in terms of effects on lactic, acetic and propionic acid metabolism, following co-incubation. Spot resistance tests showed slight but varying degrees of growth inhibition by S. bovis among the propionibacteria, while no inhibition was observed between M. elsdenii and the different strains of dairy propionibacteria. In the co-culture experiments comprising S. bovis in nutrient broth, significant differences in pH and the levels of production of lactic, acetic and propionic acid, were observed between treatments following inoculation with various propionibacteria and/or M. elsdenii. In general, lactic acid concentrations at the end of the incubation were significantly lower in the cultures containing propionibacteria compared with cultures comprising either S. bovis only or S. bovis + M. elsdenii, although efficacy of lactate metabolism varied between species and strains. Moreover,the accumulation of acetic and propionic acid in the combined cultures, but not in the solo S. bovis culture, indicated that these compounds were produced as a result of the metabolism of lactic acid by the propionibacteria and M. elsdenii.

The effect of Saccharomyces cerevisiae on in vitro growth and fermentation of Selenomonas ruminantium and Megasphaera elsdenii

2017 ◽  
Vol 13 (3) ◽  
pp. 9-22
Author(s):  
Sylwia Grochowska ◽  
Włodzimierz Nowak ◽  
Małgorzata Lasik-Kurdyś ◽  
Robert Mikuła ◽  
Jacek Nowak
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Volatile Fatty Acids ◽  
Yeast Fermentation ◽  
Yeast Culture ◽  
In Vitro Growth ◽  
Fermentation Products ◽  
Selenomonas Ruminantium ◽  
Megasphaera Elsdenii ◽  
Live Culture

Stimulation of lactate utilization by Selenomonas ruminantium and Megasphaera elsdenii may help in reducing problems associated with rumen acidosis. The objective of this study was to determine the effect of a Saccharomyces cerevisiae live culture and Saccharomyces cerevisiae fermentation products on in vitro growth and fermentation of lactate-utilizing ruminal bacteria, S. ruminantium (ATCC 19205) and M. elsdenii (ATCC 25940). The cultures were run for 0, 6, 12, 24 and 48 h under anaerobic conditions on a growth medium supplemented with a yeast live culture (SC) or with yeast fermentation products (SCFP) and, as reference, on the same medium without supplementation (CON). Neither SC nor SCFP had a significant effect on the growth of S. ruminantium after 6, 12 and 24 h of incubation, but the live yeast culture significantly (P≤0.05) improved the growth of these bacteria after 48 h of incubation. The yeast fermentation products significantly (P≤0.05) decreased pH and increased lactate synthesis by S. ruminantium. The Saccharomyces cerevisiae live culture significantly improved the growth of M. elsdenii after 12 and 24 h of incubation, and the S. cerevisiae fermentation products increased its growth after 48 h. The After 24 and 48 h of incubation the Saccharomyces cerevisiae live culture reduced the concentration of total volatile fatty acids (VFA), while caproate was the main product of in vitro fermentation of M. elsdenii (P≤0.05). Saccharomyces cerevisiae live cultures may improve microbial fibre fermentation in the rumen by maintaining optimal pH conditions.

Dairy propionibacteria as direct-fed microbials: in vitro effect on acid metabolism of Streptococcus bovis and Megasphaera elsdenii

2018 ◽  
Vol 68 (3) ◽  
pp. 153-158 ◽  
Author(s):  
Jianbiao Luo ◽  
Chaminda Senaka Ranadheera ◽  
Stuart King ◽  
Craig Andrew Evans ◽  
Surinder Baines
Keyword(s):  
Acid Metabolism ◽  
Streptococcus Bovis ◽  
Megasphaera Elsdenii ◽  
Dairy Propionibacteria ◽  
Vitro Effect

Multi-mode light microscopy of microtubule assembly dynamics and chromosome movement in vivo and In vitro

1996 ◽  
Vol 54 ◽  
pp. 730-731
Author(s):  
E. D. Salmon ◽  
J. C. Waters ◽  
C. Waterman-Storer
Keyword(s):  
Imaging System ◽  
Ccd Camera ◽  
Transmitted Light ◽  
Microtubule Assembly ◽  
Live Cells ◽  
Optical Efficiency ◽  
Multiple Band ◽  
Multi Mode

We have developed a multi-mode digital imaging system which acquires images with a cooled CCD camera (Figure 1). A multiple band pass dichromatic mirror and robotically controlled filter wheels provide wavelength selection for epi-fluorescence. Shutters select illumination either by epi-fluorescence or by transmitted light for phase contrast or DIC. Many of our experiments involve investigations of spindle assembly dynamics and chromosome movements in live cells or unfixed reconstituted preparations in vitro in which photodamage and phototoxicity are major concerns. As a consequence, a major factor in the design was optical efficiency: achieving the highest image quality with the least number of illumination photons. This principle applies to both epi-fluorescence and transmitted light imaging modes. In living cells and extracts, microtubules are visualized using X-rhodamine labeled tubulin. Photoactivation of C2CF-fluorescein labeled tubulin is used to locally mark microtubules in studies of microtubule dynamics and translocation. Chromosomes are labeled with DAPI or Hoechst DNA intercalating dyes.

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