Regulation of cAMP in a lymphocyte preparation isolated from peripheral venous blood in human subjects: the significance of residual thrombocytes, noradrenaline and prostaglandins

1998 ◽  
Vol 95 (3) ◽  
pp. 377 ◽  
Author(s):  
Susanne B. SØNDERGAARD ◽  
Jens H. KNUDSEN ◽  
Niels J. CHRISTENSEN
Keyword(s):  
Human Subjects ◽  
Venous Blood ◽  
Peripheral Venous Blood

Measurement of 5-Androsten-3β, 17β-diol in Spermatic and Peripheral Venous Blood Samples from the Same Human Subjects by a Radioimmunoassay Method

1978 ◽  
Vol 9 (4) ◽  
pp. 194-200 ◽  
Author(s):  
G. Forti ◽  
E. Calabresi ◽  
P. Giannotti ◽  
D. Borrelli ◽  
P. Gonnelli ◽  
...  
Keyword(s):  
Human Subjects ◽  
Venous Blood ◽  
Blood Samples ◽  
Peripheral Venous Blood ◽  
Radioimmunoassay Method

PRELIMINARY OBSERVATIONS ON THE RELEASE OF OXYTOCIN AND VASOPRESSIN IN HUMAN SUBJECTS

1960 ◽  
Vol 21 (1) ◽  
pp. 125-128 ◽  
Author(s):  
R. R. CHAUDHURY ◽  
G. F. JOPLIN
Keyword(s):  
Intravenous Injection ◽  
Human Subjects ◽  
Venous Blood ◽  
Blood Levels ◽  
Moderate Severity ◽  
Peripheral Venous Blood ◽  
Vasopressor Activity ◽  
Hypothalamic Damage

SUMMARY 1. Oxytocic and vasopressor activity were found in human peripheral venous blood following overnight dehydration and an intravenous injection of nicotine. 2. The quantities are adequate for investigation of hypothalamic damage of moderate severity. 3. The blood levels of oxytocic activity exceed vasopressor activity.

Regulation of cAMP in a lymphocyte preparation isolated from peripheral venous blood in human subjects: the significance of residual thrombocytes, noradrenaline and prostaglandins

1998 ◽  
Vol 95 (3) ◽  
pp. 377-383 ◽  
Author(s):  
Susanne B. SØNDERGAARD ◽  
Jens H. KNUDSEN ◽  
Niels J. CHRISTENSEN
Keyword(s):  
Healthy Subjects ◽  
Human Subjects ◽  
Venous Blood ◽  
Close Correlation ◽  
Peripheral Venous Blood ◽  
Noradrenaline Concentration ◽  
Noradrenaline And Adrenaline

1.The aim of the study was to elucidate the mechanism of the previously reported close correlation observed between noradrenaline and cAMP in a lymphocyte preparation (LP) isolated from peripheral venous blood in healthy subjects. A close correlation was also obtained in the present study between lymphocyte noradrenaline and adrenaline and cAMP both in the basal state and after stimulation with isoproterenol (P< 0.05 to 0.007). 2.Although 99% of the thrombocytes were removed from the LP during the washing procedure, LP contained approximately one thrombocyte per lymphocyte. The noradrenaline concentration in LP which could be ascribed to residual thrombocytes, calculated from the average noradrenaline concentration in thrombocytes and the number of thrombocytes in LP, correlated closely to noradrenaline in LP (P< 0.007). 3.To test the hypothesis that noradrenaline in LP was primarily derived from plasma, we studied three patients with phaeochromocytoma, who had high levels of noradrenaline and adrenaline both in plasma and in LP. 4.Further studies showed that the addition of thrombocytes to LP increased cAMP. The response was inhibited by indomethacin, whereas the addition of cimetidine and propranolol had no effect on basal or thrombocyte-stimulated cAMP. 5.We conclude that noradrenaline in LP is a marker of the number of residual thrombocytes. The addition of thrombocytes to LP increased cAMP in lymphocytes. This response was not mediated by catecholamines but possibly by prostaglandins.

Platelet Hyperaggregability in Ischemic Cerebrovascular Disease and Effects of Aspirin

1982 ◽  
Vol 48 (02) ◽  
pp. 117-119 ◽  
Author(s):  
M Kusunoki ◽  
K Kimura ◽  
K Nagatsuka ◽  
Y Isaka ◽  
O Uyama ◽  
...  
Keyword(s):  
Cerebrovascular Disease ◽  
Venous Blood ◽  
Second Phase ◽  
Cerebral Angiogram ◽  
Peripheral Venous Blood ◽  
Chronic Stage ◽  
Ischemic Cerebrovascular Disease ◽  
Carotid Arterial ◽  
Platelet Hyperaggregability ◽  
The Brain

SummaryPlatelet aggregation was studied in 24 patients in the chronic stage of ischemic cerebrovascular disease (CVD), with cerebral affluent and effluent blood, i.e., carotid arterial and internal jugular venous blood, and also with peripheral venous blood. Aggregation tests were performed at various final concentrations of sodium arachidonate (A.A.) and ADP. In 17 patients, not taking aspirin, platelet aggregability in jugular venous blood was significantly accentuated compared with that in arterial and peripheral venous blood. This tendency was more marked in the patients with cerebral artery stenosis and/or occlusion than in those with normal cerebral angiogram. In 7 patients taking 500 mg or more oral aspirin, aggregation differences across the brain were not observed and A.A. aggregation and the second phase of ADP aggregation were completely suppressed. These results suggest that a prophylactic administration of aspirin may be beneficial for patients in chronic stage of CVD.

Increased erythrocyte adhesiveness/aggregation in the peripheral venous blood of patients with ischaemic heart disease and an eventful course

2001 ◽  
Vol 56 (2) ◽  
pp. 121-126 ◽  
Author(s):  
Schlomo BERLINER ◽  
Rivka ROTSTEIN ◽  
Renato FUSMAN ◽  
Itzhak SHAPIRA ◽  
Ori ROGOWSKI ◽  
...  
Keyword(s):  
Heart Disease ◽  
Ischaemic Heart Disease ◽  
Venous Blood ◽  
Peripheral Venous Blood

Sensitive detection of circulating hepatocellular carcinoma cells in peripheral venous blood

Cancer ◽  
1995 ◽  
Vol 75 (9) ◽  
pp. 2214-2219 ◽  
Author(s):  
Toshiki Komeda ◽  
Yoshihiro Fukuda ◽  
Takehiro Sando ◽  
Ryuichi Kita ◽  
Motonobu Furukawa ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Venous Blood ◽  
Carcinoma Cells ◽  
Sensitive Detection ◽  
Hepatocellular Carcinoma Cells ◽  
Peripheral Venous Blood

Respiratory measurements of cardiac output: from elegant idea to useful test

2004 ◽  
Vol 96 (2) ◽  
pp. 428-437 ◽  
Author(s):  
Gabriel Laszlo
Keyword(s):  
Cardiac Output ◽  
Human Subjects ◽  
Venous Blood ◽  
The Body ◽  
Mixed Venous Blood ◽  
Indirect Determination ◽  
Arterial Blood ◽  
Pulmonary Capillary ◽  
Pulmonary Arterial ◽  
Mixed Venous

The measurement of cardiac output was first proposed by Fick, who published his equation in 1870. Fick's calculation called for the measurement of the contents of oxygen or CO2 in pulmonary arterial and systemic arterial blood. These values could not be determined directly in human subjects until the acceptance of cardiac catheterization as a clinical procedure in 1940. In the meanwhile, several attempts were made to perfect respiratory methods for the indirect determination of blood-gas contents by respiratory techniques that yielded estimates of the mixed venous and pulmonary capillary gas pressures. The immediate uptake of nonresident gases can be used in a similar way to calculate cardiac output, with the added advantage that they are absent from the mixed venous blood. The fact that these procedures are safe and relatively nonintrusive makes them attractive to physiologists, pharmacologists, and sports scientists as well as to clinicians concerned with the physiopathology of the heart and lung. This paper outlines the development of these techniques, with a discussion of some of the ways in which they stimulated research into the transport of gases in the body through the alveolar membrane.

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