Erythrocyte Sodium Pump Activity in Diabetes Mellitus and its Relationship to Control and Microangiopathy

1985 ◽  
Vol 69 (s12) ◽  
pp. 44P-45P
Author(s):  
P.E. Jennings ◽  
M.R. Wilkins ◽  
M.J. West ◽  
M.J. Kendall ◽  
A.H. Barnett
Keyword(s):  
Diabetes Mellitus ◽  
Sodium Pump ◽  
Pump Activity ◽  
Erythrocyte Sodium

Age and sex differences of erythrocyte sodium pump activity in normal human subjects

1977 ◽  
Vol 76 (3) ◽  
pp. 399-401 ◽  
Author(s):  
Mabel Hokin-Neaverson ◽  
David A. Spiegel ◽  
William A. Burckhardt ◽  
James W. Jefferson
Keyword(s):  
Sex Differences ◽  
Sodium Pump ◽  
Human Subjects ◽  
Pump Activity ◽  
Normal Human ◽  
Erythrocyte Sodium ◽  
Age And Sex

Relationship of Erythrocyte Sodium Pump Kinetics to Pump Activity during Pregnancy

1989 ◽  
Vol 77 (s21) ◽  
pp. 29P-29P
Author(s):  
S MacPhail ◽  
T Thomas ◽  
R Wilkindson ◽  
JM Davison ◽  
W Dunlop
Keyword(s):  
Sodium Pump ◽  
Pump Activity ◽  
Erythrocyte Sodium ◽  
Relationship Of

Deficiency of erythrocyte sodium pump activity in bipolar manic-depressive psychosis

Life Sciences ◽  
1974 ◽  
Vol 15 (10) ◽  
pp. 1739-1748 ◽  
Author(s):  
Mabel Hokin-Neaverson ◽  
David A. Spiegel ◽  
William C. Lewis
Keyword(s):  
Sodium Pump ◽  
Manic Depressive Psychosis ◽  
Manic Depressive ◽  
Pump Activity ◽  
Erythrocyte Sodium

Erythrocyte sodium pump activity in human obesity

1984 ◽  
Vol 141 (2-3) ◽  
pp. 179-187 ◽  
Author(s):  
Bambos M. Charalambous ◽  
Kevin Morgan ◽  
Gillian Spurlock ◽  
M.Afzal Mir
Keyword(s):  
Sodium Pump ◽  
Human Obesity ◽  
Pump Activity ◽  
Erythrocyte Sodium

Interaction of alcohol and kola nut on brain sodium pump activity in Wistar rats

2009 ◽  
Vol 91 (2) ◽  
pp. 357-362
Author(s):  
G.O. Obochi ◽  
A.E. Abara ◽  
S.P. Malu ◽  
M.U. Eteng ◽  
I.B. Umoh
Keyword(s):  
Wistar Rats ◽  
Sodium Pump ◽  
Pump Activity

Chlormadinone acetate, a progesterone derivative that binds to the digitalis receptor, inhibits the sodium pump in the isolated rat diaphragm

1985 ◽  
Vol 63 (1) ◽  
pp. 44-47 ◽  
Author(s):  
Ivan Bihler ◽  
Frank S. LaBella ◽  
P. C. Sawh
Keyword(s):  
Sodium Pump ◽  
Sugar Transport ◽  
Diaphragm Muscle ◽  
Rat Diaphragm ◽  
Bathing Medium ◽  
Chlormadinone Acetate ◽  
Muscle Tissues ◽  
Pump Activity ◽  
Intact Muscle ◽  
Resting Muscle

Rb+ uptake, intracellular Na+ and K+ levels, and the tissue–medium distribution of the nonmetabolized glucose analog, 3-O-methyl-D-glucose (3-MG) were measured in rat diaphragms incubated with chiormadinone acetate, 6-chloro-4,6-pregnadien-17-ol-3,20-dione 17-acetate (CMA), in the presence and absence of ouabain. CMA in concentrations of 5 × 10−7 M or higher significantly depressed 86Rb uptake, and promoted an increase in internal Na+ and a decrease in internal K+, indicating inhibition of the sodium pump. Sugar transport in resting muscle parallels the changes in internal Na+ levels and is an additional indicator of sodium pump activity. Equilibration of 3-MG between tissue and medium was accelerated by CMA, in parallel to the rise in internal Na+ level. Effects of CMA on Na+ levels and sugar transport, but not on Rb+ uptake, were additive to those of various concentrations of ouabain, suggesting interaction with sites not affected by ouabain. These results on diaphragm muscle confirm our previous studies on isolated cardiac muscle preparations showing that CMA, added to the aqueous bathing medium, inhibits the sodium pump in intact muscle tissues.

scholarly journals Inhibition of Na,K-ATPase by oleandrin and oleandrigenin, and their detection by digoxin immunoassays

1996 ◽  
Vol 42 (10) ◽  
pp. 1654-1658 ◽  
Author(s):  
S A Jortani ◽  
R A Helm ◽  
R Valdes
Keyword(s):  
Sodium Pump ◽  
Serum Proteins ◽  
Cross Reactivity ◽  
Pump Activity ◽  
Ic50 Values ◽  
On Line ◽  
Atpase Inhibition ◽  
Free Serum ◽  
Derivatives Of ◽  
Rule Out

Abstract Ingestion of oleander plant, containing the cardiac glycoside oleandrin, has been reported to induce fatal poisonings. Derivatives of oleandrin are structurally similar to digoxin. We investigated the cross-reactivities of oleandrin and its aglycone metabolite, oleandrigenin, in several commercially available digoxin immunoassays; assessed their ability to inhibit Na,K-ATPase catalytic activity; and measured their binding to proteins in serum. As assayed with ACS:180, Stratus, RIA, On-Line, and TDx digoxin assays, oleandrin at 100 micromol/L in digoxin-free serum gave apparent digoxin values of 0, 0.83, 2.24, 2.37, and 5.34 nmol/L, respectively, whereas oleandrigenin at that concentration gave results of 0, 0.52, 0.77, 4.94, and 1.40 nmol/L. Study of Na,K-ATPase inhibition showed IC50 values (micromol/L) of 0.22 for ouabain, 0.62 for oleandrin, 1.23 for oleandrigenin, and 2.69 for digoxin. At 25 degrees C, 96% of oleandrin and 48% of oleandrigenin were bound to serum proteins. Because detection of oleandrin and oleandrigenin by digoxin immunoassays is variable between assays as well as between congeners, assessment of cross-reactivity is warranted for each assay. The inhibition of Na,K-ATPase by oleandrin and oleandrigenin confirms that they likely exert their toxic effects through inhibition of sodium pump activity. In cases of digitalis-like poisoning with suspicion of oleander ingestion, a combination of digoxin immunoassays may be useful to effectively rule out the presence of oleander.

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