The effect of Arginine Vasopressin on Ureagenesis in Isolated rat Hepatocytes

1985 ◽  
Vol 69 (2) ◽  
pp. 231-233 ◽  
Author(s):  
P. J. T. Drew ◽  
J. P. Monson ◽  
H. K. Metcalfe ◽  
S. J. W. Evans ◽  
R. A. Iles ◽  
...  
Keyword(s):  
Ammonium Chloride ◽  
Dose Response ◽  
Arginine Vasopressin ◽  
Response Curve ◽  
Rat Hepatocytes ◽  
Dose Response Curve ◽  
Physiological Significance ◽  
Urea Synthesis ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

1. The effect of arginine vasopressin (AVP) on ureagenesis was measured in isolated rat hepatocytes with ammonium chloride and l(+)-lactate as substrates. 2. AVP was found to stimulate urea synthesis and the dose-response curve suggests that such an effect is present at concentrations of the hormone as low as 25-50 pmol/l. 3. Both the dose-response curve and the concentrations of NH4+ employed suggest that the effect observed could be of physiological significance.

scholarly journals Effects of phorbol esters on α1-adrenergic-mediated and glucagon-mediated actions in isolated rat hepatocytes

1985 ◽  
Vol 228 (1) ◽  
pp. 277-280 ◽  
Author(s):  
J A García-Sáinz ◽  
F Mendlovic ◽  
M A Martínez-Olmedo
Keyword(s):  
Cyclic Amp ◽  
Dose Response ◽  
Response Curve ◽  
Phorbol Esters ◽  
Rat Hepatocytes ◽  
Metabolic Effects ◽  
Isolated Rat Hepatocytes ◽  
The Right ◽  
Isolated Rat ◽  
Stimulation Of

Phorbol 12-myristate 13-acetate (PMA) inhibited the stimulation of ureogenesis produced by adrenaline, but produced a minimal displacement to the right of the dose-response curve for glucagon. However, PMA diminished the accumulation of cyclic AMP induced by glucagon. Dissociation between the cyclic AMP concentrations and the metabolic effects induced by glucagon is evidenced in the presence of phorbol esters.

scholarly journals Effect of adenosine and inosine on ureagenesis in hepatocytes

1987 ◽  
Vol 245 (2) ◽  
pp. 371-374 ◽  
Author(s):  
R Guinzberg P ◽  
I Laguna ◽  
A Zentella ◽  
R Guzman ◽  
E Piña
Keyword(s):  
Uric Acid ◽  
Rat Hepatocytes ◽  
Physiological Significance ◽  
Isolated Rat Hepatocytes ◽  
Dose Dependent ◽  
Isolated Rat ◽  
Stimulation Of

Adenosine and inosine produced a dose-dependent stimulation of ureagenesis in isolated rat hepatocytes. Hypoxanthine, xanthine and uric acid were without effect. Half-maximally effective concentrations were 0.08 microM for adenosine and 5 microM for inosine. Activation of ureagenesis by both nucleosides had the following characteristics: (a) it was observed with either glutamine or (NH4)2CO3, provided that glucose was present; (b) it was not detected when glucose was replaced by lactate plus oleate; (c) it was mutually antagonized by glucagon, but not by adrenaline; and (d) it was dependent on Ca2+. We suggest that the action of adenosine and inosine on ureagenesis might be of physiological significance.

The mechanism of inhibition of ureogenesis by acidosis

1984 ◽  
Vol 4 (10) ◽  
pp. 819-825 ◽  
Author(s):  
J. P. Monson ◽  
R. M. Henderson ◽  
J. A. Smith ◽  
R. A. Iles ◽  
M. Faus-Dader ◽  
...  
Keyword(s):  
Ammonium Chloride ◽  
Rat Liver ◽  
Rat Hepatocytes ◽  
Ph Sensitive ◽  
Perfused Rat Liver ◽  
Carbamoyl Phosphate ◽  
Isolated Rat Hepatocytes ◽  
Mechanism Of Inhibition ◽  
Cytosol Ph ◽  
Isolated Rat

In perfused rat liver a decrease of cytosol pH, determined with pH-sensitive microelectrodes7 from 7.2 to 6.85 is associated with a 50% fall in ureogenesis from ammonium chloride. In isolated rat hepatocytes the fall in ureogenesis due to acidosis is associated with decrease in the mitochondrial and cytosolic concentration of citrulline. Limitation of carbamoyl phosphate synthesis and thus citrulline supply could be responsible for the inhibition of ureogenesis observed.

Inhibition of gluconeogenesis and urea synthesis in isolated rat hepatocytes by acetazolamide

1985 ◽  
Vol 13 (1) ◽  
pp. 255-255 ◽  
Author(s):  
HILARY K. METCALFE ◽  
JOHN P. MONSON ◽  
PETER J. DREW ◽  
RICHARD A. ILES ◽  
NICHOLAS D. CARTER ◽  
...  
Keyword(s):  
Rat Hepatocytes ◽  
Urea Synthesis ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

The Effect of Arginine Vasopressin on Ureogenesis in Isolated Rat Hepatocytes

1985 ◽  
Vol 68 (s11) ◽  
pp. 48P-49P
Author(s):  
P.J.T. Drew ◽  
H.K. Metcalfe ◽  
J.P. Monson ◽  
R.A. Iles ◽  
R.D. Cohen
Keyword(s):  
Arginine Vasopressin ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

Glucagon degradation in isolated rat hepatocytes: Effect of ammonium chloride and chloroquine

1981 ◽  
Vol 23 (3) ◽  
pp. 311-320 ◽  
Author(s):  
Bertrand Canivet ◽  
Phillip Gorden ◽  
Jean-Louis Carpentier ◽  
Lelio Orci ◽  
Pierre Freychet
Keyword(s):  
Ammonium Chloride ◽  
Rat Hepatocytes ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat
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