scholarly journals Effects of phorbol esters on α1-adrenergic-mediated and glucagon-mediated actions in isolated rat hepatocytes

1985 ◽  
Vol 228 (1) ◽  
pp. 277-280 ◽  
Author(s):  
J A García-Sáinz ◽  
F Mendlovic ◽  
M A Martínez-Olmedo
Keyword(s):  
Cyclic Amp ◽  
Dose Response ◽  
Response Curve ◽  
Phorbol Esters ◽  
Rat Hepatocytes ◽  
Metabolic Effects ◽  
Isolated Rat Hepatocytes ◽  
The Right ◽  
Isolated Rat ◽  
Stimulation Of

Phorbol 12-myristate 13-acetate (PMA) inhibited the stimulation of ureogenesis produced by adrenaline, but produced a minimal displacement to the right of the dose-response curve for glucagon. However, PMA diminished the accumulation of cyclic AMP induced by glucagon. Dissociation between the cyclic AMP concentrations and the metabolic effects induced by glucagon is evidenced in the presence of phorbol esters.

scholarly journals Stimulatory effect of the intestinal peptide PHI on glycogenolysis and gluconeogenesis in isolated rat hepatocytes

1983 ◽  
Vol 214 (3) ◽  
pp. 999-1002 ◽  
Author(s):  
J E Felíu ◽  
J Marco
Keyword(s):  
Cyclic Amp ◽  
Pyruvate Kinase ◽  
Glycogen Phosphorylase ◽  
Rat Hepatocytes ◽  
Fold Increase ◽  
Isolated Rat Hepatocytes ◽  
Mediated Effects ◽  
Dose Dependent ◽  
Isolated Rat ◽  
Stimulation Of

The newly isolated peptide PHI provoked a dose-dependent stimulation of glycogenolysis and gluconeogenesis in isolated rat hepatocytes; at 1 microM-PHI, both processes were increased 1.6-fold as compared with basal values. These PHI-mediated effects were accompanied by the activation of glycogen phosphorylase and the inactivation of pyruvate kinase. PHI (1 microM) also caused a 2-fold increase in hepatocyte cyclic AMP.

The effect of Arginine Vasopressin on Ureagenesis in Isolated rat Hepatocytes

1985 ◽  
Vol 69 (2) ◽  
pp. 231-233 ◽  
Author(s):  
P. J. T. Drew ◽  
J. P. Monson ◽  
H. K. Metcalfe ◽  
S. J. W. Evans ◽  
R. A. Iles ◽  
...  
Keyword(s):  
Ammonium Chloride ◽  
Dose Response ◽  
Arginine Vasopressin ◽  
Response Curve ◽  
Rat Hepatocytes ◽  
Dose Response Curve ◽  
Physiological Significance ◽  
Urea Synthesis ◽  
Isolated Rat Hepatocytes ◽  
Isolated Rat

1. The effect of arginine vasopressin (AVP) on ureagenesis was measured in isolated rat hepatocytes with ammonium chloride and l(+)-lactate as substrates. 2. AVP was found to stimulate urea synthesis and the dose-response curve suggests that such an effect is present at concentrations of the hormone as low as 25-50 pmol/l. 3. Both the dose-response curve and the concentrations of NH4+ employed suggest that the effect observed could be of physiological significance.

scholarly journals Histamine H2 receptors on foetal-bovine articular chondrocytes

1983 ◽  
Vol 212 (2) ◽  
pp. 517-520 ◽  
Author(s):  
D J Taylor ◽  
J R Yoffe ◽  
D E Woolley
Keyword(s):  
Cyclic Amp ◽  
Adenylate Cyclase ◽  
Dose Response ◽  
Response Curve ◽  
Articular Chondrocytes ◽  
Monolayer Cultures ◽  
H2 Receptors ◽  
Bovine Articular Chondrocytes ◽  
The Right ◽  
H1 Antagonists

The dose-response curve of histamine-induced cyclic AMP elevation in monolayer cultures of primary foetal-bovine articular chondrocytes was displaced to the right by cimetidine. In addition, H2 but not H1 antagonists prevented the histamine-induced cyclic AMP elevation, suggesting histamine activates chondrocyte adenylate cyclase through an H2 receptor.

scholarly journals The mechanism by which adenosine decreases gluconeogenesis from lactate in isolated rat hepatocytes

1987 ◽  
Vol 246 (2) ◽  
pp. 449-454 ◽  
Author(s):  
A Lavoinne ◽  
H A Buc ◽  
S Claeyssens ◽  
M Pinosa ◽  
F Matray
Keyword(s):  
Ketone Body ◽  
Adenine Nucleotides ◽  
Rat Hepatocytes ◽  
Adenosine Kinase ◽  
Isolated Rat Hepatocytes ◽  
Adenosine Deaminase 2 ◽  
Body Production ◽  
Gluconeogenic Substrates ◽  
Isolated Rat ◽  
Stimulation Of

Incubation of hepatocytes from 24 h-starved rats in the presence of 0.5 mM-adenosine decreased gluconeogenesis from lactate, but not from alanine. The inhibition of gluconeogenesis was associated with a stimulation of ketone-body production and an inhibition of pyruvate oxidation. These metabolic changes were suppressed in the presence of iodotubercidin (an inhibitor of adenosine kinase), but were reinforced in the presence of deoxycoformycin (an inhibitor of adenosine deaminase); 2-chloroadenosine induced no change in gluconeogenesis from lactate. These data indicate that the inhibition of gluconeogenesis by adenosine probably results from its conversion into adenine nucleotides. In the presence of lactate or pyruvate, but not with alanine or asparagine, this conversion resulted in a decrease in the [ATP]/[ADP] ratio in both mitochondrial and cytosolic compartments. Adenosine decreased the Pi concentration with all gluconeogenic substrates.

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