The Effects of Plasma from Normal Subjects Taking Low and High Salt Diets on the Erythrocyte Sodium Pump

1983 ◽  
Vol 65 (3) ◽  
pp. 66P-66P
Author(s):  
P.L. Weissberg ◽  
M.J. West ◽  
M.R. Wilkins
Keyword(s):  
Sodium Pump ◽  
Normal Subjects ◽  
High Salt ◽  
Erythrocyte Sodium

Relationship between the Maximum Velocity and Sodium Affinity of the Erythrocyte Sodium Pump and its Rate Constant in Blood

1990 ◽  
Vol 79 (6) ◽  
pp. 625-630 ◽  
Author(s):  
T. H. Thomas ◽  
S. MacPhail ◽  
V. Mott ◽  
R. Wilkinson
Keyword(s):  
Rate Constant ◽  
Whole Blood ◽  
Sodium Pump ◽  
Maximum Velocity ◽  
Normal Subjects ◽  
Affinity Constant ◽  
Pump Rate ◽  
Median Error ◽  
Data Points ◽  
Erythrocyte Sodium

1. Young and mature erythrocytes from 15 normal subjects were used to compare the sodium pump rate constant measured in whole blood with the more definitive sodium affinity constant and maximum velocity of the sodium pump measured in artificial media using sodium-loaded cells. 2. Similar values were obtained from both erythrocyte fractions for the sodium affinity constant and maximum velocity and also by using two different plots. The median error in the estimate of individual sodium affinity constants and maximum velocities from regression analysis was about 20% and the precision was not improved by combining the data points for the two erythrocyte fractions. 3. The rate constant in whole blood was closely related to the sodium affinity constant and maximum velocity of the sodium pump (r = 0.75), suggesting that it was a reasonable overall assessment of available sodium pump activity. 4. Differences in the rate constant between subjects were due to differences in both the maximum velocity and sodium affinity constant of the sodium pump so that the rate constant could not be used as a guide to the underlying sodium pump physiology.

Factors affecting Potassium Balance During Frusemide Administration

1984 ◽  
Vol 67 (2) ◽  
pp. 195-203 ◽  
Author(s):  
Christopher S. Wilcox ◽  
William E. Mitch ◽  
Ralph A. Kelly ◽  
Paul A. Friedman ◽  
Paul F. Souney ◽  
...  
Keyword(s):  
Salt Intake ◽  
Normal Subjects ◽  
Plasma Aldosterone ◽  
High Salt ◽  
Renin Angiotensin Aldosterone System ◽  
Plasma Aldosterone Concentration ◽  
Water Load ◽  
Renin Angiotensin ◽  
High Salt Intake ◽  
Low Salt

1. We investigated the effects of Na+ intake, the renin-angiotensin-aldosterone system and antidiuretic hormone (ADH) on K+ balance during 3 days of frusemide administration to six normal subjects. Subjects received 40 mg of frusemide for 3 days during three different protocols: Na+ intake 270 mmol/day (high salt); Na+ intake 20 mmol/day to stimulate the renin-angiotensin-aldosterone system (low salt); Na+ intake 270 mmol/day plus captopril (25 mg/6 h) to prevent activation of the renin-angiotensin-aldosterone system. In a fourth protocol, a water load was given during high salt intake to prevent ADH release and then frusemide was given. 2. During high salt intake, frusemide increased K+ excretion (UKV) over 3 h, but the loss was counterbalanced by subsequent renal K+ retention so that daily K+ balance was neutral. 3. During low salt intake, the magnitude of the acute kaliuresis following the first dose of frusemide and the slope of the linear relationship between UKV and the log of frusemide excretion were increased compared with that found during the high salt intake. In addition, low salt intake abolished the compensatory renal retention of K+ after frusemide and cumulative K+ balance over 3 days of diuretic administration was uniformly negative (−86 ± 7 mmol/3 days; P < 0.001). 4. Captopril abolished the rise in plasma aldosterone concentration induced by frusemide. The acute kaliuresis after frusemide was unchanged compared with that observed during high salt intake. The compensatory reduction in UKV occurring after the diuretic was slightly potentiated. In fact, captopril given without the diuretic induced a small positive K+ balance. 5. When a water load was given concurrently with frusemide, the acute kaliuresis was >30% lower compared with that seen with frusemide alone, even though the natriuretic response was unchanged. 6. We conclude that: (a) K+ balance is maintained when frusemide is given during liberal Na+ intake because acute K+ losses are offset by subsequent renal K+ retention; (b) this compensatory K+ retention can be inhibited by aldosterone release which could account for the negative K+ balance seen during salt restriction; (c) the short-term kaliuretic response to frusemide is augmented by release of both ADH and aldosterone whereas changes in K+ balance over 3 days of frusemide are dependent on plasma aldosterone concentration.

Is the Erythrocyte Sodium Pump Altered in Human Obesity?*

1983 ◽  
Vol 56 (5) ◽  
pp. 925-929 ◽  
Author(s):  
Bruce M. Simat ◽  
Robert R. Mayrand ◽  
Arthur H. L. From ◽  
John E. Morley ◽  
Charles Billington ◽  
...  
Keyword(s):  
Sodium Pump ◽  
Human Obesity ◽  
Erythrocyte Sodium

A serial study of erythrocyte sodium pump kinetics and sodium content in the puerperium

1994 ◽  
Vol 170 (2) ◽  
pp. 693-698 ◽  
Author(s):  
Lesley M. Bolton ◽  
Trevor H. Thomas ◽  
Sheila Macphail ◽  
William Dunlop
Keyword(s):  
Sodium Pump ◽  
Sodium Content ◽  
Erythrocyte Sodium

Lithium and erythrocyte membrane cation carrier studies in normal and manic depressive subjects

1977 ◽  
Vol 7 (2) ◽  
pp. 229-233 ◽  
Author(s):  
G. J. Naylor ◽  
A. Smith ◽  
L. J. Boardman ◽  
D. A. T. Dick ◽  
E. G. Dick ◽  
...  
Keyword(s):  
Erythrocyte Membrane ◽  
Human Subjects ◽  
Normal Subjects ◽  
Sodium Concentration ◽  
Manic Depressive Psychosis ◽  
Manic Depressive ◽  
Normal Human ◽  
Potassium Influx ◽  
Erythrocyte Sodium

synopsisChanges in the erythrocyte membrane cation carrier following lithium ingestion in normal human subjects were studied; ouabain sensitive potassium influx fell significantly during the lithium treated phase. Lithium was fed to rats and no change in erythrocyte Na-K ATPase was shown. These findings contrast with studies of lithium in manic depressive psychosis. The fluctuations in the erythrocyte membrane cation carrier were studied in 5 normal subjects over 12 weeks and the correlations between the parameters calculated. The erythrocyte sodium concentration correlated positively with the ouabain sensitive potassium influx. This too contrasts with findings in manic depressive psychosis.

Erythrocyte Sodium Pump Activity in Diabetes Mellitus and its Relationship to Control and Microangiopathy

1985 ◽  
Vol 69 (s12) ◽  
pp. 44P-45P
Author(s):  
P.E. Jennings ◽  
M.R. Wilkins ◽  
M.J. West ◽  
M.J. Kendall ◽  
A.H. Barnett
Keyword(s):  
Diabetes Mellitus ◽  
Sodium Pump ◽  
Pump Activity ◽  
Erythrocyte Sodium

Age and sex differences of erythrocyte sodium pump activity in normal human subjects

1977 ◽  
Vol 76 (3) ◽  
pp. 399-401 ◽  
Author(s):  
Mabel Hokin-Neaverson ◽  
David A. Spiegel ◽  
William A. Burckhardt ◽  
James W. Jefferson
Keyword(s):  
Sex Differences ◽  
Sodium Pump ◽  
Human Subjects ◽  
Pump Activity ◽  
Normal Human ◽  
Erythrocyte Sodium ◽  
Age And Sex

Relationship of Erythrocyte Sodium Pump Kinetics to Pump Activity during Pregnancy

1989 ◽  
Vol 77 (s21) ◽  
pp. 29P-29P
Author(s):  
S MacPhail ◽  
T Thomas ◽  
R Wilkindson ◽  
JM Davison ◽  
W Dunlop
Keyword(s):  
Sodium Pump ◽  
Pump Activity ◽  
Erythrocyte Sodium ◽  
Relationship Of
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