Application of a new method for the sensitive detection and quantification of acyl-CoA esters in Arabidopsis thaliana seedlings and mature leaves

2000 ◽  
Vol 28 (6) ◽  
pp. 575-577 ◽  
Author(s):  
T. R. Larson ◽  
I. A. Graham
Keyword(s):  
Arabidopsis Thaliana ◽  
Reversed Phase ◽  
New Method ◽  
Sensitive Detection ◽  
Reversed Phase Hplc ◽  
Selective Method ◽  
Mature Leaves ◽  
Highly Sensitive ◽  
First Time ◽  
Detection And Quantification

We report a novel, highly sensitive and selective method for the detection and quantification of acyl-CoA esters from Arabidopsis thaliana. Extracted acyl-CoA esters were derived to their fluorescent acyl-etheno-CoA esters, separated by ion-paired reversed-phase HPLC, and detected fluorometrically. We report the composition and concentration of the acyl-CoA pool in mature A. thaliana leaves, and during storage-lipid breakdown in 2-day-old seedlings. The concentrations measured were in the 1–4 μM range for both tissue types. To our knowledge, this is the first time that reliable estimates of acyl-CoA ester concentrations have been made for plants.

A H2O2-free electrochemical peptide biosensor based on Au@Pt bimetallic nanorods for highly sensitive sensing of matrix metalloproteinase 2

2020 ◽  
Vol 56 (45) ◽  
pp. 6039-6042 ◽  
Author(s):  
Xiaoxue Xi ◽  
Meiqi Wen ◽  
Shihao Song ◽  
Junlun Zhu ◽  
Wei Wen ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Sensitive Detection ◽  
Matrix Metalloproteinase 2 ◽  
Highly Sensitive ◽  
Bimetallic Nanorods ◽  
Highly Sensitive Detection ◽  
First Time

We construct for the first time a H2O2-free electrochemical peptide biosensor based on Au@Pt bimetallic nanorods in neutral substrate solutions for highly sensitive detection of matrix metalloproteinase 2.

Deposition of nanoplastics in high-altitude Alpine snow

2020 ◽  
Author(s):  
Dusan Materic ◽  
Elke Ludewig ◽  
Simona M. Cristescu ◽  
Thomas Röckmann ◽  
Rupert Holzinger
Keyword(s):  
High Altitude ◽  
Environmental Samples ◽  
Common Type ◽  
New Method ◽  
Methodological Issues ◽  
Remote Areas ◽  
Surface Snow ◽  
Daily Record ◽  
First Time ◽  
Detection And Quantification

<p>Recent research discovered that airborne microplastics pollution can reach very remote areas. However, analysis of nanoplastics in environmental samples remains challenging, mostly due to technical and methodological issues. A new method for chemical characterisation of nanoplastics based on TD-PTR-MS has been recently introduced. The detection limit of <1ng allowed for the first time the analysis of nanoplastics deposited on the snow in the pristine Alps. In this work, we analysed daily samples of surface snow close to the Sonnblick Observatory, Austria (3106 m altitude) in the period from 2017-02-07 to 2017-03-19, using our new method. The results showed a positive detection for various types of nanoplastics, and the most common type found was Polyethylene terephthalate (PET). We will present our results on the to-date longest daily record of nanoplastics deposition in high altitude regions and further discuss optimisations of TD-PTR-MS method for nanoplastics detection and quantification.</p>

scholarly journals Flavonoids from Lonchocarpus muehlbergianus

2004 ◽  
Vol 76 (4) ◽  
pp. 651-661 ◽  
Author(s):  
Aderbal F. Magalhães ◽  
Ana M.G.A. Tozzi ◽  
Eva G. Magalhães ◽  
Ivani S. Blanco ◽  
Maria-Del-Pilar C. Soriano
Keyword(s):  
Quantitative Analysis ◽  
Spectral Data ◽  
13C Nmr ◽  
2D Nmr ◽  
Reversed Phase ◽  
Light Petroleum ◽  
Natural Occurrence ◽  
Reversed Phase Hplc ◽  
First Time

The light petroleum extract from the roots of Lonchocarpus muehlbergianus Hassl contained nine flavonoids, including six new ones. These are 2,4-cis-2,4,5,8-tetramethoxy-(2´´,3´´:6,7)-furanoflavan; 2,4-cis-4-hydroxy-2,5,8-trimethoxy-(2´´,3´´:6,7)-furanoflavan; 2,4-cis-2-prenyloxy-4,5,8-trimethoxy-(2´´,3´´:6,7)-fu-ranoflavan; 2,4-cis-2-prenyloxy-4-hydroxy-5,8-dimethoxy-(2´´,3´´:6,7)-furanoflavan; 2',5',6'-trimethoxy-9-(1,1-dimethylallyoxy)-[2´´,3´´:3´,4´]-furanochalcone; 5,6-dimethoxy-(2´´,3´´:7,8)-furanoflavone, identi-fied by analysis of their spectral data (UV, IR, ¹H and 13C NMR, 2D-NMR, NOE and MS). The natural occurrence of 2,4-dioxygenated flavan derivatives is being reported for the first time. Quantitative analysis of the petrol extract, by using reversed-phase HPLC, showed that the most abundant flavonoid in the extract is 2,4-cis-2,4,5,8- tetramethoxy-(2´´,3´´:6,7)-furanoflavan.

Fluorescence behavior of a unique two-photon fluorescent probe in aggregate and solution states and highly sensitive detection of RNA in water solution and living systems

2016 ◽  
Vol 52 (57) ◽  
pp. 8838-8841 ◽  
Author(s):  
Yong Liu ◽  
Fangfang Meng ◽  
Longwei He ◽  
Xiaoqiang Yu ◽  
Weiying Lin
Keyword(s):  
Fluorescent Probe ◽  
Water Solution ◽  
Pure Water ◽  
Water Systems ◽  
Living Systems ◽  
Sensitive Detection ◽  
Two Photon ◽  
Highly Sensitive ◽  
Highly Sensitive Detection ◽  
First Time

In this work, we developed a unique fluorescent probe HVC-6 for highly sensitive detection of RNA in pure water systems and living systems by aggregation–disaggregation of the probe for the first time.

scholarly journals Determination of ciprofloxacin in plasma and urine by HPLC with ultraviolet detection

1998 ◽  
Vol 44 (6) ◽  
pp. 1251-1255 ◽  
Author(s):  
Marika Kamberi ◽  
Kimiko Tsutsumi ◽  
Tsutomu Kotegawa ◽  
Koichi Nakamura ◽  
Shigeyuki Nakano
Keyword(s):  
Plasma Proteins ◽  
Reversed Phase ◽  
Pharmacokinetic Studies ◽  
Reversed Phase Hplc ◽  
Ultraviolet Detection ◽  
Efficient Procedure ◽  
Quantification Limit ◽  
Detection And Quantification ◽  
Phase Column

Abstract A simple, sensitive isocratic method for the detection and quantification of ciprofloxacin in plasma and urine has been developed. The assay consisted of reversed-phase HPLC with ultraviolet detection. Plasma proteins were removed by a fast and efficient procedure. For the urine samples, the only required sample preparation was dilution. Separation was achieved on a C18 reversed-phase column. The quantification limit was 0.01 mg/L in plasma and 0.5 mg/L in urine. This method was sufficiently sensitive for pharmacokinetic studies.

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