Excision-Repair of 4-Nitroquinoline 1-Oxide-Induced Deoxyribonucleic Acid Damage in Human-Embryo Intestinal and Lung Cells

1979 ◽  
Vol 7 (6) ◽  
pp. 1248-1249
Author(s):  
WILLIAM W. SELWAY ◽  
WILLIAM J. HARRIS
Keyword(s):  
Human Embryo ◽  
Deoxyribonucleic Acid ◽  
Excision Repair ◽  
Lung Cells

Permeabilization of ultraviolet-irradiated Chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus

1981 ◽  
Vol 1 (3) ◽  
pp. 237-244
Author(s):  
D B Yarosh ◽  
R B Setlow
Keyword(s):  
Polyethylene Glycol ◽  
Deoxyribonucleic Acid ◽  
Excision Repair ◽  
Micrococcus Luteus ◽  
Chinese Hamster ◽  
Endonuclease Activity ◽  
Chinese Hamster Cells ◽  
Ultraviolet Resistance

Chinese hamster V-79 cells were made permeable by treatment with polyethylene glycol and then incubated with a Micrococcus luteus extract containing ultraviolet-specific endonuclease activity. This treatment introduced nicks in irradiated, but not in unirradiated, deoxyribonucleic acid. The nicks remained open for at least 3 h; there was no loss of endonuclease-sensitive sites, and no excision of dimers as measured by chromatography was detected. In addition, there was no increase in ultraviolet resistance in treated cells. This suggests that the absence of a significant amount of excision repair in rodent cells is due to the lack of both incision and excision capacity.

scholarly journals Permeabilization of ultraviolet-irradiated Chinese hamster cells with polyethylene glycol and introduction of ultraviolet endonuclease from Micrococcus luteus.

1981 ◽  
Vol 1 (3) ◽  
pp. 237-244 ◽  
Author(s):  
D B Yarosh ◽  
R B Setlow
Keyword(s):  
Polyethylene Glycol ◽  
Deoxyribonucleic Acid ◽  
Excision Repair ◽  
Micrococcus Luteus ◽  
Chinese Hamster ◽  
Endonuclease Activity ◽  
Chinese Hamster Cells ◽  
Ultraviolet Resistance

Chinese hamster V-79 cells were made permeable by treatment with polyethylene glycol and then incubated with a Micrococcus luteus extract containing ultraviolet-specific endonuclease activity. This treatment introduced nicks in irradiated, but not in unirradiated, deoxyribonucleic acid. The nicks remained open for at least 3 h; there was no loss of endonuclease-sensitive sites, and no excision of dimers as measured by chromatography was detected. In addition, there was no increase in ultraviolet resistance in treated cells. This suggests that the absence of a significant amount of excision repair in rodent cells is due to the lack of both incision and excision capacity.

Formation and excision of covalent deoxyribonucleic acid adducts of benzo[a]pyrene 4,5-epoxide and benzo[a]pyrenediol epoxide I in human lung cells A549

Biochemistry ◽  
1980 ◽  
Vol 19 (6) ◽  
pp. 1095-1101 ◽  
Author(s):  
George Feldman ◽  
Joyce Remsen ◽  
Tzu-Chien V. Wang ◽  
Peter Cerutti
Keyword(s):  
Deoxyribonucleic Acid ◽  
Human Lung ◽  
Lung Cells ◽  
Human Lung Cells

Correlation Between Deoxyribonucleic Acid Excision-Repair and Life-Span in a Number of Mammalian Species

2001 ◽  
Vol 2001 (1) ◽  
Author(s):  
R. W. Hart ◽  
R. B. Setlow
Keyword(s):  
Dna Synthesis ◽  
Life Span ◽  
Uv Irradiation ◽  
Deoxyribonucleic Acid ◽  
Initial Rate ◽  
Excision Repair ◽  
Mammalian Species ◽  
Unscheduled Dna Synthesis

The ability of fibroblasts to perform unscheduled DNA synthesis (a measure of excision repair) after ultraviolet (UV) irradiation was measured radioautographically for seven species at several times after several UV fluences. Both the initial rate and the maximum incorporation of [′H]dThd increased with the life-span of the species (shrew, mouse, rat, hamster, cow, elephant, and human). Unscheduled DNA synthesis was approximately proportional to the logarithm of life-span. Reproduced by permission. R. W. Hart, R. B. Setlow, Correlation Between Deoxyribonucleic Acid Excision-Repair and Life-Span in a Number of Mammalian Species. Proc. Natl. Acad. Sci. U.S.A. 71 , 2169-2173 (1974).

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