The origin of human embryo lung cells in culture: A comment on cell differentiation, in vitro growth and neoplasia

2006 ◽  
Vol 9 (1) ◽  
pp. 19-29 ◽  
Author(s):  
L. M. Franks ◽  
T. W. Cooper
Keyword(s):  
Cell Differentiation ◽  
Human Embryo ◽  
Lung Cells ◽  
In Vitro Growth ◽  
Cells In Culture

scholarly journals TIMING OF DNA SYNTHESIS IN THE MITOTIC CYCLE IN VITRO

1961 ◽  
Vol 9 (3) ◽  
pp. 509-518 ◽  
Author(s):  
Jesse E. Sisken ◽  
Riojun Kinosita
Keyword(s):  
Dna Synthesis ◽  
Generation Time ◽  
Mitotic Cycle ◽  
Tritiated Thymidine ◽  
Lung Cells ◽  
Human Amnion ◽  
Cells In Culture ◽  
Single Colony ◽  
Distribution Of Values

A study was made of the timing of DNA synthesis in the mitotic cycle under conditions where the average mitotic cycle of populations of human amnion and kitten lung cells in culture was variable. Three types of experiments were performed: (a) Autoradiographs were made of incorporated tritiated thymidine in cells whose mitotic histories were recorded microcinematographically allowing the measurement of telophase + G1 along with the total length of the mitotic cycle. (b) Measurement of the G2 + prophase part of the mitotic cycle was performed under various conditions by exposing cells to tritiated thymidine and observing the increase in labeled metaphases plus anaphases as a function of time. (c) The effect of a change in pH on parts of the mitotic cycle was tested by continuously photographing a single colony of cells first at pH 7.8 and then at pH 7.1. All of our data point to the same conclusion; namely, that within a population of cells with a given generation time, the length of each of the measurable parts of the mitotic cycle has a particular distribution of values and that, when there is a change in the generation time, under our conditions only the T + G1 distribution changes.

THE IN VITRO GROWTH OF RUBELLA VIRUS IN HUMAN EMBRYO CELLS1

1965 ◽  
Vol 81 (1) ◽  
pp. 71-85 ◽  
Author(s):  
STANLEY A. PLOTKIN ◽  
ANDRE BOUÉ ◽  
JOELLE G. BOUÉ
Keyword(s):  
Rubella Virus ◽  
Human Embryo ◽  
In Vitro Growth

In vitro growth characteristics of rat mesothelioma cells in culture

1986 ◽  
Vol 57 (3) ◽  
pp. 249-256 ◽  
Author(s):  
M. C. Jaurand ◽  
A. Renier ◽  
A. Meeren ◽  
L. Magne ◽  
M. C. Pinchon ◽  
...  
Keyword(s):  
Growth Characteristics ◽  
In Vitro Growth ◽  
Cells In Culture

Effect of in Vivo Oxidized Cellulose on in Vitro Growth of Human Respiratory Mucosa and Sub-mucosa During Endoscopic Skull Base Approaches

2016 ◽  
Vol 77 (S 01) ◽  
Author(s):  
Ezequiel Goldschmidt ◽  
Jorge Rasmussen ◽  
Joseph Chabot ◽  
Monica Loressi ◽  
Marcelo Ielpi ◽  
...  
Keyword(s):  
Skull Base ◽  
Oxidized Cellulose ◽  
In Vitro Growth ◽  
Respiratory Mucosa

In-vitro- und In-vivo-Wirkung von Schilddrüsenhormon auf das Wachstum von Neuroblastomzellen

1990 ◽  
Vol 29 (03) ◽  
pp. 120-124
Author(s):  
R. P. Baum ◽  
E. Rohrbach ◽  
G. Hör ◽  
B. Kornhuber ◽  
E. Busse
Keyword(s):  
Cell Differentiation ◽  
Neuroblastoma Cells ◽  
Control Group ◽  
Initial Value ◽  
Initial Rise ◽  
Biphasic Effect ◽  
Contrast Microscopy ◽  
Morphological Sign

The effect of triiodothyronine (T3) on the differentiation of cultured neuroblastoma (NB) cells was studied after 9 days of treatment with a dose of 10-4 M/106 cells per day. Using phase contrast microscopy, 30-50% of NB cells showed formation of neurites as a morphological sign of cellular differentiation. The initial rise of the mitosis rate was followed by a plateau. Changes in cyclic nucleotide content, in the triphosphates and in the activity of the enzyme ornithine decarboxylase (ODC) were assessed in 2 human and 2 murine cell lines to serve as biochemical parameters of the cell differentiation induced by T3. Whereas the cAMP level increased significantly (3 to 7 fold compared with its initial value), the cGMP value dropped to 30 to 50% of that of the control group. ATP and GTP increased about 200%, the ODC showed a decrease of about 50%. The present studies show a biphasic effect of T3 on neuroblastoma cells: the initial rise of mitotic activity is followed by increased cell differentiation starting from day 4 of the treatment.

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