scholarly journals Pathophysiological changes in the cerebellum and brain stem in a rabbit model after superior petrosal vein sacrifice

2018 ◽  
Vol 38 (6) ◽  
Author(s):  
Lei Cheng ◽  
Pin Guo ◽  
Yi-wei Liao ◽  
Hong-liang Zhang ◽  
Huan-ting Li ◽  
...  
Keyword(s):  
Brain Stem ◽  
Rabbit Model ◽  
Control Group ◽  
Stem Tissue ◽  
Nissl Staining ◽  
Petrosal Vein ◽  
Time Period ◽  
Cerebellar Tissue ◽  
Superior Petrosal Vein ◽  
The Brain

In certain surgical procedures, sacrificing the superior petrosal vein (SPV) is required. Previous studies have reported transient cerebellar edema, venous infarction, or hemorrhage that might occur after sectioning of the SPV. The present study investigated the pathophysiological changes in cerebellum and brain stem after SPV sacrifice. Rabbits were divided into the operation group where the SPV was sacrificed and the control group where the SPV remained intact. Each group was further subdivided into 4, 8, 12, 24, 48, and 72 h groups which represented the time period from sacrificing of the SPV to killing of the rabbits. The water content (WC), Na+ content, K+ content, and pathophysiological changes in cerebellum and brain stem tissue were measured. In comparison with the control, the WC and Na+ content of cerebellar tissue were increased in the 4, 8, 12, and 24 h operation subgroups (P<0.05), but only increased in the 4-h subgroup of the brain stem tissue (P<0.05). The K+ content of the cerebellar tissue decreased in the 4, 8, 12, and 24 h operation subgroups (P<0.05) but only decreased in the 4-h subgroup of brain stem tissue (P<0.05). Nissl staining and TEM demonstrated that cerebellar edema occurred in the 4, 8, 12, and 24 h operation subgroups but not in the 48- and 72-h subgroups. Brain stem edema occurred in the 4-h operation subgroup. In summary, cerebellum and brain stem edema can be observed at different time points after sacrificing of the SPV in the rabbit model.

scholarly journals Role of Nitric Oxide in Regulation of Brain Stem Circulation during Hypotension

1997 ◽  
Vol 17 (10) ◽  
pp. 1089-1096 ◽  
Author(s):  
Kazunori Toyoda ◽  
Kenichiro Fujii ◽  
Setsuro Ibayashi ◽  
Tetsuhiko Nagao ◽  
Takanari Kitazono ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Brain Stem ◽  
Topical Application ◽  
Basilar Artery ◽  
Group Versus ◽  
Control Group ◽  
Cranial Window ◽  
Severe Hypotension ◽  
The Brain

We tested the hypothesis that nitric oxide (NO) plays a role in CBF autoregulation in the brain stem during hypotension. In anesthetized rats, local CBF to the brain stem was determined with laser-Doppler flowmetry, and diameters of the basilar artery and its branches were measured through an open cranial window during stepwise hemorrhagic hypotension. During topical application of 10−5 mol/L and 10−4 mol/L Nω-nitro-L-arginine (L-NNA), a nonselective inhibitor of nitric oxide synthase (NOS), CBF started to decrease at higher steps of mean arterial blood pressure in proportion to the concentration of L-NNA in stepwise hypotension (45 to 60 mm Hg in the 10−5 mol/L and 60 to 75 mm Hg in the 10−4 mol/L L-NNA group versus 30 to 45 mm Hg in the control group). Dilator response of the basilar artery to severe hypotension was significantly attenuated by topical application of L-NNA (maximum dilatation at 30 mm Hg: 16 ± 8% in the 10−5 mol/L and 12 ± 5% in the 10−4 mol/L L-NNA group versus 34 ± 4% in the control group), but that of the branches was similar between the control and L-NNA groups. Topical application of 10−5 mol/L 7-nitro indazole, a selective inhibitor of neuronal NOS, did not affect changes in CBF or vessel diameter through the entire pressure range. Thus, endothelial but not neuronal NO seems to take part in the regulation of CBF to the the brain stem during hypotension around the lower limits of CBF autoregulation. The role of NO in mediating dilatation in response to hypotension appears to be greater in large arteries than in small ones.

scholarly journals Comparison of Auditory Brain Stem Responses and Otoacoustic Emission of Autism with Healthy Children

2020 ◽  
Vol 9 ◽  
pp. 1937
Author(s):  
Seyed Gholamreza Noorazar ◽  
Yalda Jabbari Moghaddam ◽  
Rasul Kharzaee ◽  
Mojtaba Sohrabpour
Keyword(s):  
Brain Stem ◽  
Rating Scale ◽  
Children With Autism ◽  
Otoacoustic Emission ◽  
Repetitive Behaviors ◽  
Control Group ◽  
Healthy Children ◽  
Auditory Brain Stem Responses ◽  
Auditory Brain Stem ◽  
The Brain

Background: Autism is a neurodevelopment disorder, including difficulty in establishing relationships and social interaction, difficulty in communication, performing restricted, and repetitive behaviors. The impaired reception and integration of sensory information especially auditory data are one of the main characteristics of children with autism.  According to various studies, the brain stem plays a key role in the reception and integration of auditory and sensory data. Hence, this study aims to comparison auditory brain stem responses (ABR) and otoacoustic emission (OAE) of autism patients with healthy children. Materials and Methods: This case-control study was performed on 20 autism children (4-8 years old) as case group who referred to psychiatry clinics affiliated with Tabriz University of Medical Sciences and 20 healthy age-matched as the control group. The severity of autism was evaluated by the Gilliam Autism Rating Scale (GARS). Also, ABR and OAE were recorded, and all data compared with the healthy children. Results: The latencies between the waves III-V and I-V bilaterally, and wave V bilaterally and wave I in the left ear showed a significant increase in children with autism compared to the healthy group. Conclusion: This study shows that there was a reduced nerve conduction velocity in the auditory pathway of the brain stem in children with autism compared to healthy children. [GMJ.2020;9:e1937]

scholarly journals Automated Segmentation of Cerebellum Using Brain Mask and Partial Volume Estimation Map

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Dong-Kyun Lee ◽  
Uicheul Yoon ◽  
Kichang Kwak ◽  
Jong-Min Lee
Keyword(s):  
Brain Stem ◽  
Similarity Index ◽  
Volume Estimation ◽  
Morphological Operations ◽  
Cerebellar Tissue ◽  
Morphological Operator ◽  
Cerebellar Peduncle ◽  
The Individual ◽  
The Brain ◽  
Dice Similarity Index

While segmentation of the cerebellum is an indispensable step in many studies, its contrast is not clear because of the adjacent cerebrospinal fluid, meninges, and cerebra peduncle. Thus, various cerebellar segmentation methods, such as a deformable model or a template-based algorithm might exhibit incorrect segmentation of the venous sinuses and the cerebellar peduncle. In this study, we propose a fully automated procedure combining cerebellar tissue classification, a template-based approach, and morphological operations sequentially. The cerebellar region was defined approximately by removing the cerebral region from the brain mask. Then, the noncerebellar region was trimmed using a morphological operator and the brain-stem atlas was aligned to the individual brain to define the brain-stem area. The proposed method was validated with the well-known FreeSurfer and ITK-SNAP packages using the dice similarity index and recall and precision scores. As a result, the proposed method was significantly better than the other methods for the dice similarity index (0.93, FreeSurfer: 0.92, ITK-SNAP: 0.87) and precision (0.95, FreeSurfer: 0.90, ITK-SNAP: 0.93). Therefore, it could be said that the proposed method yielded a robust and accurate segmentation result. Moreover, additional postprocessing with the brain-stem atlas could improve its result.

scholarly journals Effects of sevoflurane exposure during different stages of pregnancy on the brain development of rat offspring

2021 ◽  
Author(s):  
Feng-he Cui ◽  
Jie Li ◽  
Ke-zhong Li ◽  
Yong-gang Xie ◽  
Xiao-ling Zhao
Keyword(s):  
Brain Development ◽  
Late Pregnancy ◽  
Control Group ◽  
Sevoflurane Anesthesia ◽  
Nissl Staining ◽  
Learning Capacity ◽  
Rat Offspring ◽  
Tnf Α ◽  
Natural Birth ◽  
The Brain

Abstract Objective This study explored the effects of sevoflurane exposure during different stages of pregnancy on the brain development of offspring. Methods Thirty-six pregnant SD rats were randomly divided into 4 groups: control, sevoflurane exposure in early (S1) pregnancy, sevoflurane exposure in middle (S2) pregnancy, and sevoflurane exposure in late (S3) pregnancy. After natural birth, the learning and memory capacity of offspring rats was analyzed using the Morris water maze experiment. The hippocampi of offspring rats were collected. The levels of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α in the hippocampus were measured by ELISA. Additionally, the Nissl bodies in the hippocampus were analyzed using Nissl staining. Immunohistochemistry was used to examine the expression of BDNF and CPEB2 in the hippocampus of offspring. Proteins related to the NR4A1/NF-κB pathway were analyzed using western blotting. Results The memory and learning capacity of offspring rats was significantly reduced in the S1 and S2 groups compared to the control group (p < 0.05), while there was no obvious difference between the control and S3 groups (p > 0.05). The level of IL-1β was significantly increased (p < 0.05) in the S1 group compared with the control group. Sevoflurane anesthesia received in early and middle pregnancy could significantly affect the formation of Nissl bodies in the hippocampi of offspring rats. In addition, the expression of BDNF and CPEB2 in the hippocampi of offspring rats was greatly decreased in the S1 group compared with the control group (p < 0.05). The expression of NR4A1 in the hippocampi of rat offspring was significantly decreased in the S1 and S2 groups compared with the control group (p < 0.05). The expression of proteins related to the NF-κB pathway was increased in the S1 group compared to the control group (p < 0.05). Conclusions The neurotoxic effect of maternal sevoflurane anesthesia on the brain development of offspring is higher when the exposure occurs in early pregnancy than in late pregnancy, and its mechanism might involve the NR4A1/NF-κB pathway to increase the secretion of inflammatory cytokines.

scholarly journals Electroacupuncture Improves Clearance of Amyloid-β through the Glymphatic System in the SAMP8 Mouse Model of Alzheimer’s Disease

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Pei-zhe Liang ◽  
Li Li ◽  
Ya-nan Zhang ◽  
Yan Shen ◽  
Li-li Zhang ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Cognitive Impairment ◽  
Neurodegenerative Disorder ◽  
Amyloid Β ◽  
Control Group ◽  
Nissl Staining ◽  
Glymphatic System ◽  
Model Of Alzheimer’S Disease ◽  
The Brain

Background. Memory loss and cognitive impairment characterize the neurodegenerative disorder, Alzheimer’s disease (AD). Amyloid-β (Aβ) is the key factor that triggers the course of AD, and reducing the deposition of Aβ in the brain has been considered as a potential target for the treatment of AD. In clinical and animal studies, electroacupuncture (EA) has been shown to be an effective treatment for AD. In recent years, substantial evidence has accumulated suggesting the important role of the glymphatic system in Aβ clearance. Objective. The purpose of this study was to explore whether EA modifies the accumulation of Aβ through the glymphatic system and may thus be applied to alleviate cognitive impairments. Methods. Seven-month-old SAMP8 mice were randomized into a control group (Pc) and an electroacupuncture group (Pe). Age-matched SAMR1 mice were used as normal controls (Rc). Mice in the Pe group were stimulated on Baihui (GV20) and Yintang (GV29) for 10 min and then pricked at Shuigou (GV26) for ten times. EA treatment lasted for 8 weeks. In each week, EA would be applied once a day for the first five consecutive days and ceased at the remaining two days. After EA treatment, Morris water maze (MWM) test was used to evaluate the cognitive function; HE and Nissl staining was performed to observe the brain histomorphology; ELISA, contrast-enhanced MRI, and immunofluorescence were applied to explore the mechanisms underlying EA effects from Aβ accumulation, glymphatic system function, reactivity of astrocytes, and AQP4 polarization, respectively. Results. This EA regime could improve cognition and alleviate neuropathological damage to brain tissue. And EA treatment might reduce Aβ accumulation, enhance paravascular influx in the glymphatic system, inhibit the reactivity of astrocytes, and improve AQP4 polarity. Conclusion. EA treatment might reduce Aβ accumulation from the brain via improving clearance performance of the glymphatic system and thereby alleviating cognitive impairment.

scholarly journals Stereological Evaluation of the Brains in Patients with Parkinson’s disease Compared to Controls

2017 ◽  
Vol 25 (3) ◽  
pp. 265-274 ◽  
Author(s):  
Zahra Heidari ◽  
Ali Moghtaderi ◽  
Hamidreza Mahmoudzadeh-Sagheb ◽  
Enam Alhagh Charkhat Gorgich
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Brain Stem ◽  
Volume Density ◽  
Control Group ◽  
Middle Cerebellar Peduncle ◽  
Significance Level ◽  
Significant Difference ◽  
Cerebellar Peduncle ◽  
The Brain

Abstract Parkinson’s disease (PD) is a chronic and progressive neurological disorder. A tetrad of bradykinesia, rigidity, tremor and postural instability are the core features of the disease. The aim of this study was to evaluate stereological changes in the brain of patients with PD and compare them with that of healthy controls. This case-control study was conducted on 29 patients with PD and 12 controls (C) in Zahedan, Iran. All subjects enrolled into the study through the convenience sampling method. MRI images of the brains of two groups in frontal and sagittal axis with consecutive 5mm distance slices were captured. Parameters including total volume (V) and volume density (Vv) of different parts of the brain were estimated based on Cavalries’ point counting stereological method. To analyze the data, descriptive statistics, Mann-Whitney U-Test applied for comparing the PD and C groups were used. Significance level was set at p<0.05. Our study showed that the volume of the brain and total volume and volume density (Vv) of cerebral hemispheres, cerebellum, ventricles, hippocampus, pons, mid brain and superior cerebellar peduncles in the PD group did not indicate significant difference from the control group. Total volume of brain stem in PD group wasn’t significantly different from the control group. The volume density of brain stem (p= 0.012) and total volume and volume density of middle cerebellar peduncle (p< 0.0001) in PD group were significantly larger than the control group. This study shows that PD stereological parameters related to volume and volume density of middle cerebellar peduncle and volume density of brain stem were significantly larger in patients compared to the controls. Therefore, stereological parameters can be used for early diagnosis and probably for follow-up in patients with PD.

Fos-like immunoreactivity in the brain stem following oral quinine stimulation in decerebrate rats

1999 ◽  
Vol 277 (2) ◽  
pp. R384-R394 ◽  
Author(s):  
Joseph B. Travers ◽  
Kevin Urbanek ◽  
Harvey J. Grill
Keyword(s):  
Brain Stem ◽  
Control Group ◽  
Solitary Tract ◽  
Intact Group ◽  
Sensorimotor Processing ◽  
Unstimulated Control ◽  
Rejection Response ◽  
Lower Brain Stem ◽  
The Brain ◽  
Intact Rats

The present study compared the distribution of Fos-like immunoreactivity (FLI) following intraoral stimulation with quinine monohydrochloride (QHCl) in awake intact rats to the pattern obtained in chronic supracollicular decerebrate (CD) rats. Because the behavioral rejection response to QHCl is evident in the CD rat, it was hypothesized that the pattern of FLI in the lower brain stem should be similar in both groups. Overall, the distribution of FLI in the brain stem was quite similar in both intact and CD groups, and QHCl stimulation increased FLI in the rostral (gustatory) nucleus of the solitary tract, the parabrachial nucleus (PBN), and the lateral reticular formation (RF) compared with an unstimulated control group. The CD group differed from the intact group, however, with a trend toward less FLI in the RF and a shift in the pattern of label away from the external subdivision of the PBN. CD rats also had increased FLI in the caudal nucleus of the solitary tract, with or without intraoral infusions. The distribution of QHCl-induced FLI in the brain stem of intact rats thus indicates both local sensorimotor processing as well as the influence of forebrain structures.

scholarly journals THE POSSIBILITIES OF COMPuTEd TOMOGRAPHY IN ASSESSING THE RISK OF STROKE

2017 ◽  
Vol 9 (4) ◽  
pp. 35-38
Author(s):  
M A Kotov ◽  
K I Sebelev ◽  
R O Terent’ev
Keyword(s):  
Computed Tomography ◽  
Blood Flow ◽  
High Risk ◽  
Brain Stem ◽  
Morphological Changes ◽  
Diagnostic Methods ◽  
Control Group ◽  
Stem Density ◽  
Stroke Outcomes ◽  
The Brain

Stroke occurs when the blood flow in the brain tissues decreases, causing morphological changes - microcirculation disturbances leading to a change in the density of the substance, which can be visualized using radiation diagnostic methods and based on the data obtained, to identify patients at high risk of stroke. The purpose of this study is to study density of brain stem structures in patients with different stroke outcomes compared to the control group. When analyzing these indicators, a correlation was found between changes in brain stem density and the likelihood of a stroke.

Permeability of the microcirculation in area postrema of rat

1988 ◽  
Vol 46 ◽  
pp. 348-349
Author(s):  
Shams M. Ghoneim ◽  
Frank M. Faraci ◽  
Gary L. Baumbach
Keyword(s):  
Brain Stem ◽  
Area Postrema ◽  
Upper Body ◽  
Sprague Dawley ◽  
Sodium Cacodylate ◽  
Acute Hypertension ◽  
Sprague Dawley Rats ◽  
Ultrastructural Characteristics ◽  
The Brain ◽  
Adjacent Brain

The area postrema is a circumventricular organ in the brain stem and is one of the regions in the brain that lacks a fully functional blood-brain barrier. Recently, we found that disruption of the microcirculation during acute hypertension is greater in area postrema than in the adjacent brain stem. In contrast, hyperosmolar disruption of the microcirculation is greater in brain stem. The objective of this study was to compare ultrastructural characteristics of the microcirculation in area postrema and adjacent brain stem.We studied 5 Sprague-Dawley rats. Horseradish peroxidase was injected intravenously and allowed to circulate for 1, 5 or 15 minutes. Following perfusion of the upper body with 2.25% glutaraldehyde in 0.1 M sodium cacodylate, the brain stem was removed, embedded in agar, and chopped into 50-70 μm sections with a TC-Sorvall tissue chopper. Sections of brain stem were incubated for 1 hour in a solution of 3,3' diaminobenzidine tetrahydrochloride (0.05%) in 0.05M Tris buffer with 1% H2O2.

Surgical Approaches to the Brain Stem

1993 ◽  
Vol 4 (3) ◽  
pp. 457-468 ◽  
Author(s):  
Dennis Y. Wen ◽  
Roberto C. Heros
Keyword(s):  
Brain Stem ◽  
Surgical Approaches ◽  
The Brain
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