scholarly journals Oligosaccharides obtained from a blood-group-Sd(a+) Tamm-Horsfall glycoprotein. An n.m.r. study

1986 ◽  
Vol 236 (3) ◽  
pp. 821-828 ◽  
Author(s):  
A S R Donald ◽  
J Feeney
Keyword(s):  
Blood Group ◽  
Bacteroides Fragilis ◽  
Two Dimensional ◽  
Methylation Analysis ◽  
Constant Proportion ◽  
Enzyme Digest ◽  
Complete Assignment ◽  
Beta Galactosidase ◽  
Urinary Glycoprotein

Treatment of Tamm-Horsfall urinary glycoprotein with Bacteroides fragilis endo-beta-galactosidase over a range of enzyme concentrations, pH and temperature resulted in the release of a small but constant proportion of the terminal sugars, which indicates the presence in the glycoprotein of relatively few enzyme-susceptible -GlcNAc beta 1-3Gal beta 1-4GlcNAc- units. Three oligosaccharides were isolated from the enzyme digest and characterized as Gal beta 1-4GlcNAc beta 1-3Gal, NeuAc alpha 2-3Gal beta 1-4 GlcNAc beta 1-3Gal and GalNAc beta 1-4(NeuAc alpha 2-3)Gal beta 1-4GlcNAc beta 1-3Gal by methylation analysis and exo-glycosidase digestion. The alditols of these oligosaccharides and related structures were examined by 500 MHz 1H-n.m.r. spectroscopy aided by spin-spin decoupling and two-dimensional correlated spectroscopy. An almost complete assignment of proton shifts was possible, and significant differences between the signals of some of the protons in the blood-group-Sda-active oligosaccharide III and literature values for the corresponding signals in the structurally related Cad-blood-group determinant are noted.

scholarly journals 500 MHz 1 H n.m.r. of oligosaccharides of N-acetyl-lactosamine-type released from human erythrocyte glycopeptides by endo-β-galactosidase

1988 ◽  
Vol 250 (1) ◽  
pp. 9-13 ◽  
Author(s):  
E F Hounsell ◽  
J Feeney ◽  
P Scudder
Keyword(s):  
Blood Group ◽  
Human Erythrocyte ◽  
Bacteroides Fragilis ◽  
Structural Studies ◽  
Beta Galactosidase

500 MHz 1H n.m.r. spectroscopy has been used in structural studies of three linear and five branched oligosaccharides of N-acetyl-lactosamine-type that were released from desialylated blood group O erythrocyte glycopeptides by treatment with the endo-beta-galactosidase of Bacteroides fragilis followed by reduction. The following oligosaccharide alditols were characterized: (formula; see book)

scholarly journals Determination of the structure of a novel acidic oligosaccharide with blood-group activity isolated from bovine submaxillary-gland mucin

1989 ◽  
Vol 260 (2) ◽  
pp. 389-393 ◽  
Author(s):  
S M D'Arcy ◽  
C M Donoghue ◽  
C A M Koeleman ◽  
D H Van den Eijnden ◽  
A V Savage
Keyword(s):  
Blood Group ◽  
Submaxillary Gland ◽  
Two Dimensional ◽  
Methylation Analysis ◽  
One Dimensional ◽  
First Report ◽  
Novel Structure ◽  
Blood Group H

In this study we investigated the structure of an acidic fucose-containing pentasaccharide released from bovine submaxillary-gland mucin by alkaline-borohydride treatment. The structure, determined by a combination of one-dimensional and two-dimensional 1H-n.m.r. spectroscopy at 270 MHz and methylation analysis involving g.l.c.-m.s., was as follows: Fuc alpha(1-2)Gal beta(1-4)GlcNAc beta(1-3)[NeuAc alpha(2-6)]GalNAcol This pentasaccharide is a novel structure and is the first report of a blood-group-H type 2 determinant on a submaxillary-gland mucin.

A One- and Two-Dimensional 1H and 13C N.M.R. Study of Some Fernene Triterpenoids

1989 ◽  
Vol 42 (7) ◽  
pp. 1185 ◽  
Author(s):  
AL Wilkins ◽  
JA Elix ◽  
AG Gonzalez ◽  
C Perez
Keyword(s):  
Two Dimensional ◽  
Methyl Group ◽  
Complete Assignment

A complete assignment of the 1H and 13C n.m.r. signals of the lichen triterpenoid 12α-acetoxyfern-9(11)-en-3 β-ol (lb) has been achieved by using one- and two-dimensional n.m.r. techniques. Evidence in support of one of two recently advanced assignments of the methyl group resonances of fern-9(11)-en-3-β-ol (1a) is presented.

Narcissus Alkaloids, XIII. Complete Assignment of the Nmr Spectra of Papyramine and 6-epi-Papyramine by Two-Dimensional Nmr Spectroscopy

1990 ◽  
Vol 53 (6) ◽  
pp. 1456-1462 ◽  
Author(s):  
Jaume Bastida ◽  
Carles Codina ◽  
Francesc Viladomat ◽  
Mario Rubiralta ◽  
Jean-Charles Quirion ◽  
...  
Keyword(s):  
Nmr Spectroscopy ◽  
Nmr Spectra ◽  
Two Dimensional ◽  
Complete Assignment

The structure of the antigenic lipopolysaccharide O-chain produced by Escherichiahermannii ATCC 33650 and 33652

1990 ◽  
Vol 68 (8) ◽  
pp. 1456-1466 ◽  
Author(s):  
Linda M. Beynon ◽  
David R. Bundle ◽  
Malcolm B. Perry
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
High Resolution ◽  
Molecular Modelling ◽  
Partial Hydrolysis ◽  
Two Dimensional ◽  
Methylation Analysis ◽  
Structure Formula ◽  
Structural Methods ◽  
Nuclear Magnetic

High resolution two-dimensional 1H and 13C nuclear magnetic resonance at 500 MHz was used in combination with molecular modelling to solve the structures of the antigenic O-polysaccharides produced by Escherichiahermannii strains ATCC 33650 and 33652. Classical structural methods such as methylation analysis, selective and partial hydrolysis, and periodate oxidations confirmed that the O-polysaccharides had a branched tetrasaccharide repeating unit with the structure:[Formula: see text]Keywords: Escherichiahermannii, lipopolysaccharide, magnetic resonance, polysaccharide.

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