scholarly journals Combinatorial protein engineering of proteolytically resistant mesotrypsin inhibitors as candidates for cancer therapy

2016 ◽  
Vol 473 (10) ◽  
pp. 1329-1341 ◽  
Author(s):  
Itay Cohen ◽  
Olumide Kayode ◽  
Alexandra Hockla ◽  
Banumathi Sankaran ◽  
Derek C. Radisky ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Protein Engineering ◽  
Morbidity And Mortality ◽  
New Therapies ◽  
Combinatorial Protein Engineering ◽  
Pancreatic Cancers ◽  
The Way

Cancer is a leading cause of morbidity and mortality worldwide. The results presented in the present study pave the way to develop new therapies targeting mesotrypsin, an enzyme that contributes to progression and metastasis of lung, prostate, breast and pancreatic cancers.

Staphylococcal display for combinatorial protein engineering of a head-to-tail affibody dimer binding the Alzheimer amyloid-β peptide

2012 ◽  
Vol 8 (1) ◽  
pp. 139-145 ◽  
Author(s):  
Hanna Lindberg ◽  
Anna Johansson ◽  
Torleif Härd ◽  
Stefan Ståhl ◽  
John Löfblom
Keyword(s):  
Protein Engineering ◽  
Amyloid Β ◽  
Amyloid Β Peptide ◽  
Combinatorial Protein Engineering

Structure-based Combinatorial Protein Engineering (SCOPE)

2002 ◽  
Vol 321 (4) ◽  
pp. 677-691 ◽  
Author(s):  
Paul E. O'Maille ◽  
Marina Bakhtina ◽  
Ming-Daw Tsai
Keyword(s):  
Protein Engineering ◽  
Combinatorial Protein Engineering

scholarly journals Radiation-Induced Heart Disease: A Clinical Update

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Syed Wamique Yusuf ◽  
Shehzad Sami ◽  
Iyad N. Daher
Keyword(s):  
Radiation Therapy ◽  
Heart Disease ◽  
Cardiovascular Diseases ◽  
Cancer Therapy ◽  
Cancer Survivors ◽  
Cardiovascular Effects ◽  
Cardiac Complication ◽  
Morbidity And Mortality ◽  
Radiation Induced ◽  
Effects Of Radiation

Cardiovascular diseases and cancer are the two leading causes of morbidity and mortality worldwide. Improvement in cancer therapy has led to increasing number of cancer survivors, some of whom may suffer from adverse cardiovascular effects of radiation therapy. Longterm followup is essential, as the cardiac complication may manifest years after completion of radiation therapy. In this paper, we have discussed the cardiovascular effects of radiation therapy.

scholarly journals Directed Evolution of Therapeutic Antibodies Targeting Glycosylation in Cancer

Cancers ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 2824
Author(s):  
Ron Amon ◽  
Ronit Rosenfeld ◽  
Shahar Perlmutter ◽  
Oliver C. Grant ◽  
Sharon Yehuda ◽  
...  
Keyword(s):  
Cancer Therapy ◽  
Therapeutic Efficacy ◽  
Surface Display ◽  
Yeast Surface Display ◽  
Structural Basis ◽  
Therapeutic Antibodies ◽  
Pancreatic Cancers ◽  
Yeast Surface ◽  
Directed Molecular Evolution ◽  
Cancer Theranostics

Glycosylation patterns commonly change in cancer, resulting in expression of tumor-associated carbohydrate antigens (TACA). While promising, currently available anti-glycan antibodies are not useful for clinical cancer therapy. Here, we show that potent anti-glycan antibodies can be engineered to acquire cancer therapeutic efficacy. We designed yeast surface display to generate and select for therapeutic antibodies against the TACA SLea (CA19−9) in colon and pancreatic cancers. Elite clones showed increased affinity, better specificity, improved binding of human pancreatic and colon cancer cell lines, and increased complement-dependent therapeutic efficacy. Molecular modeling explained the structural basis for improved antibody functionality at the molecular level. These new tools of directed molecular evolution and selection for effective anti-glycan antibodies, provide insights into the mechanisms of cancer therapy targeting glycosylation, and provide major methodological advances that are likely to open up innovative avenues of research in the field of cancer theranostics.

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