scholarly journals The effects of 1-hydroxyethane-1,1-diphosphonate and dichloromethanediphosphonate on collagen synthesis by rabbit articular chondrocytes and rat bone cells

1981 ◽  
Vol 196 (1) ◽  
pp. 293-301 ◽  
Author(s):  
H L Guenther ◽  
H E Guenther ◽  
H Fleisch
Keyword(s):  
Collagen Synthesis ◽  
Articular Chondrocytes ◽  
Bone Cells ◽  
Radioactive Material ◽  
Secondary Culture ◽  
Positive Effect ◽  
Rabbit Articular Chondrocytes ◽  
Isolated Rat ◽  
Rat Bone

Investigations were performed to assess the effects of dichloromethanediphosphonate on the synthesis of collagen by (1) isolated rabbit articular chondrocytes, (2) isolated rat calvaria bone cells and (3) bone explants from rats treated with the diphosphonates. The studies showed that dichloromethanediphosphonate, but not 1-hydroxyethane-1,1-diphosphonate, causes articular chondrocytes to increase net collagen biosynthesis, both when measured as 3H-labelled or as non-radioactive material, in a dose-related fashion. The increment in collagen synthesis was still evident with cells that were exposed continuously to the diphosphonate in primary as well as secondary culture; however, it declined with cells in tertiary culture and was absent after the fourth subculture. The type of collagen was not affected by the diphosphonate. The synthesis of collagen by bone cells was likewise increased with dichloromethanediphosphonate. No effects were detected with 1-hydroxyethane-1,1-diphosphonate was tested. Finally, when calvaria and tibiae from diphosphonate-treated rats were cultured in vitro, the positive effect of dichloromethanediphosphonate on collagen synthesis was also evident. 1-Hydroxyethane-1,1-diphosphonate, on the other hand, decreased the incorporation of [3H]proline into the collagen of calvaria and osseous tibial shafts and showed no effect on the collagen synthesis of the cartilaginous tibial heads.

Effects of Glucocorticoids on Fetal Rat Bone Collagen Synthesis in Vitro*

Endocrinology ◽  
1979 ◽  
Vol 104 (3) ◽  
pp. 715-721 ◽  
Author(s):  
JOHN W. DIETRICH ◽  
ERNESTO M. CANALIS ◽  
DONNA M. MAINA ◽  
LAWRENCE G. RAISZ
Keyword(s):  
Collagen Synthesis ◽  
Bone Collagen ◽  
Fetal Rat ◽  
Bone Collagen Synthesis ◽  
Rat Bone

Defective stimulation of proliferation and collagen biosynthesis of human bone cells by serum from diabetic patients

1992 ◽  
Vol 127 (6) ◽  
pp. 509-514 ◽  
Author(s):  
Rolf E Brenner ◽  
Bert Riemenschneider ◽  
Werner Blum ◽  
Martin Mörike ◽  
Walter M Teller ◽  
...  
Keyword(s):  
Metabolic Control ◽  
Collagen Synthesis ◽  
Bone Cells ◽  
Cell Attachment ◽  
Cell Number ◽  
Human Bone ◽  
Diabetic Patients ◽  
Inhibitory Influence ◽  
Control Serum

We studied the influence of fasting serum from nine insulin-dependent diabetic children and adolescents under insufficient metabolic control on normal human bone cells in vitro compared with serum from eight sex- and age-matched controls. Cell number 24 h after plating was significantly less under diabetic serum, indicating impaired cell attachment, spreading and initiation of cell proliferation. Cell number after five days was reduced by 1% diabetic serum, while higher serum concentrations had diverging effects on osteoblast proliferation. Collagen synthesis of human osteoblasts was significantly reduced by 8% diabetic serum compared to 8% control serum, while synthesis of non-collagenous proteins was not affected. Duration of diabetes (several weeks up to 12 years) had no influence on these parameters. The serum from one patient, which was studied a second time under excellent metabolic control three months later, however, had lost its inhibitory influence on collagen synthesis of osteoblasts. The pattern of the interstitial collagen types I, III and V was not altered by diabetic serum. These results indicate that defective regulation of proliferation and collagen synthesis of osteoblasts by components present in human diabetic serum may be an important factor in the development of diabetic osteopenia. The negative influence might be explained in part by reduced levels of IGF-I and elevated levels of IGF binding protein-1 in the diabetic sera.

scholarly journals Inorganic pyrophosphate release by rabbit articular chondrocytes in vitro

1986 ◽  
Vol 29 (12) ◽  
pp. 1485-1492 ◽  
Author(s):  
A. Pieter A. Prins ◽  
Erno Kiljan ◽  
Rob J. van de Stadt ◽  
Jan K. van der Korst
Keyword(s):  
Articular Chondrocytes ◽  
Inorganic Pyrophosphate ◽  
Rabbit Articular Chondrocytes

IN VITRO STUDIES ON COLLAGEN METABOLISM IN METAPHYSEAL RAT BONE

1975 ◽  
Vol 80 (4) ◽  
pp. 775-783 ◽  
Author(s):  
Norvald Langeland
Keyword(s):  
Amino Acid ◽  
Collagen Synthesis ◽  
Treated Group ◽  
In Vitro Studies ◽  
Collagen Metabolism ◽  
Bone Collagen ◽  
Accretion Rates ◽  
Different Doses ◽  
Rat Bone

ABSTRACT The effect of oestradiol-17β treatment on bone collagen metabolism in vitro was studied in metaphyseal rat bone. Rats were oophorectomized and subsequently treated for 3 weeks with different doses of oestradiol-17β. Bone pieces were incubated in a modified Krebs-Ringer bicarbonate medium for 6 h. Synthesis of [14C] hydroxyproline from [14C]proline and the incorporation of this amino acid into the bone samples was determined and the collagen synthesis and accretion rates calculated from these data. Collagen resorption rates were calculated from measured release of non-radioactive hydroxyproline to the medium. Castration resulted in an increased rate of accretion and resorption of collagen. All doses of oestradiol tested in this study (1 to 20 μg per animal per day for 3 weeks) decreased both accretion and resorption rates to levels insignificantly different from those of the non-castrated control rats. Only the 2 μg treated group had significantly better collagen balance than the castrated untreated rats.

scholarly journals Effects of anti-inflammatory drugs on matrix degradation of rabbit articular chondrocytes in vitro.

Ensho ◽  
1986 ◽  
Vol 6 (1) ◽  
pp. 71-73
Author(s):  
Katsuhiro Shimada ◽  
Masayuki Shinmei ◽  
Toshiyuki Kikuchi ◽  
Koichi Masuda ◽  
Yutaka Shimomura ◽  
...  
Keyword(s):  
Articular Chondrocytes ◽  
Matrix Degradation ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Rabbit Articular Chondrocytes
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