scholarly journals Phospholipase A activity associated with membranes of human polymorphonuclear leucocytes

1977 ◽  
Vol 167 (3) ◽  
pp. 839-841 ◽  
Author(s):  
R Franson ◽  
J Weiss ◽  
L Martin ◽  
J K Spitznagel ◽  
P Elsbach
Keyword(s):  
Cytoplasmic Granule ◽  
Phospholipase A ◽  
Polymorphonuclear Leucocytes ◽  
Total Alkaline Phosphatase ◽  
Total Alkaline Phosphatase Activity ◽  
Optimal Activity ◽  
Granule Fraction ◽  
Human Granulocytes ◽  
Membrane Bound ◽  
Total Alkaline

Homogenates of human polymorphonuclear leucocytes (granulocytes) contain a Ca2+-dependent phospholipase A with optimal activity pH7.0. This enzyme is membrane-bound and is enriched in crude cytoplasmic-granule fraction. Ratezonal centrifugation of the cytoplasmic-granule fraction demonstrates that the phospholipase A is associated not only with specific- and azurophilic-granule populations but also with an ‘empty’ vesicular fraction containing 85% of the total alkaline phosphatase activity of whole homogenate. Thus this phospholipase is associated with granule as well as with other cellular membranes of human granulocytes.

scholarly journals Release of alkaline phosphatase from membranes by a phosphatidylinositol-specific phospholipase C.

1977 ◽  
Vol 167 (1) ◽  
pp. 281-284 ◽  
Author(s):  
M G Low ◽  
J B Finean
Keyword(s):  
Staphylococcus Aureus ◽  
Alkaline Phosphatase ◽  
Phospholipase C ◽  
Mammalian Species ◽  
Salt Solutions ◽  
Total Alkaline Phosphatase ◽  
Total Alkaline Phosphatase Activity ◽  
Wide Range ◽  
Releasing Effect ◽  
Total Alkaline

Purified phosphatidylinositol-specific phospholipase C from Staphylococcus aureus released a substantial proportion of the total alkaline phosphatase activity from a wide range of tissues from several mammalian species. Co-purification of the phospholipase C and alkaline phosphatase-releasing activities and the inhibition of both these activities by iso-osmotic salt solutions suggested that the releasing effect was unlikely to be due to a contaminant.

scholarly journals A Comparison of the Effects of Resistance and Endurance Training Protocols on Serum Irisin Level and Alkaline Phosphatase Activity in Sedentary Obese Women

2020 ◽  
Vol 27 (4) ◽  
pp. 23-28
Author(s):  
Fereshte Poutafkand ◽  
Hamid Marefati ◽  
Hossein Taherichadorneshin
Keyword(s):  
Alkaline Phosphatase ◽  
Exercise Training ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Obese Women ◽  
Total Alkaline Phosphatase ◽  
Resistance Exercise Training ◽  
Total Alkaline Phosphatase Activity ◽  
Total Alkaline ◽  
Training Protocols

Abstract Introduction. Studies have revealed that the anabolic effect of irisin on bone is mediated by an increase in alkaline phosphatase. However, few studies have investigated the interactive effect of irisin on alkaline phosphatase after exercise training. Therefore, the present study aimed to compare the impact of endurance and resistance training protocols on serum irisin concentration and total alkaline phosphatase activity in sedentary obese women. Material and methods. Forty-five sedentary obese women (age: 48.96 ± 5.2 years, body mass index 32.24 ± 3.76 kg/m2) were randomly assigned to control, endurance, and resistance groups. Endurance (45 to 75 minutes at an intensity corresponding to 50 to 80% of heart rate reserve) and resistance exercise training (3 sets, 10-15 repetitions at an intensity corresponding to 50 to 65% of one-repetition maximum) were conducted for 8 weeks, 3 days per week. Maximal oxygen consumption (VO2max) was estimated using the modified Bruce protocol treadmill test. Fasting blood samples were taken before the first and 48-hr after the last exercise training sessions. The serum concentrations of irisin and total alkaline phosphatase activity were measured using the sandwich ELISA method and photo-metric method, respectively. Results. Both endurance and resistance exercise training protocols caused a significant reduction in BMI and BFP of obese women. In contrast, VO2max significantly increased after both exercise training protocols. However, neither endurance nor resistance training protocols had a significant impact on the serum concentrations of irisin and total alkaline phosphatase activity. No significant inter-group differences were observed between the subjects’ BMI, BFP, VO2max, total alkaline phosphatase, and irisin at the end of protocols. Conclusions. The finding of the current study revealed that neither of the training protocols had a significant impact on bone anabolic parameters. However, performing these types of exercise is suggested for weight management in obese women.

scholarly journals Total Alkaline Phosphatase Activity in Water of Rusalka Lake in the City of Szczecin

2013 ◽  
Vol 12 ◽  
pp. 60-66
Author(s):  
Piotr Daniszewski
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Total Alkaline Phosphatase ◽  
Total Alkaline Phosphatase Activity ◽  
Level Of Activity ◽  
Catchment Areas ◽  
Water Catchment ◽  
The City ◽  
Total Alkaline

The progress of civilizations causes changes in the water catchment areas lakes; aquatic waters are order - phosphorus and nitrogen compounds. Level of activity alkaline phosphatase (APA) has an important impact on the chemical and biological processes taking place in the aquatic environment. APA belongs to a group of indicators, who tells us about the extent limitations to development of biomass in the tank water. Water of Rusalka lake were the subjects of 2008-2012 (July – September). Total alkaline phosphatase activity was determined seven times a year in these environments. A study of seasonal fluctuations showed that a maximum total alkaline phosphatase activity, both in the water and in the bottom sediment, occurred in spring (May) and summer (July, August).

scholarly journals Total Alkaline Phosphatase Activity of Water in the Miedwie Lake (North-West Poland) on the Basis of the European Union Water Framework Directive

2013 ◽  
Vol 15 ◽  
pp. 213-217
Author(s):  
Piotr Daniszewski
Keyword(s):  
European Union ◽  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
The European Union ◽  
Seasonal Fluctuations ◽  
Total Alkaline Phosphatase ◽  
Total Alkaline Phosphatase Activity ◽  
North West ◽  
Total Alkaline

Water in the Miedwie Lake were the subjects of a five-year study (2008-2010). Total alkaline phosphatase activity was determined seven times a year in these environments. The study on the water demonstrated that the top sublittoral layer (1 m) featured the highest alkaline phosphatase activity among all the analyzed zones. A study of seasonal fluctuations showed that a maximum total alkaline phosphatase activity, both in the water occurred in spring (May) and summer (July, August). Basing on this parameter no increase in eutrophication process in the Miedwie Lake was determined in the course of a 3-year study.

Quality Control of an Automated Differential Isoenzyme Assay of Alkaline Phosphatase, with Use of L-Phenylalanine Inhibition

1972 ◽  
Vol 18 (4) ◽  
pp. 391-392 ◽  
Author(s):  
Fred Cantor ◽  
Sidney Green ◽  
Leo L Stolbach ◽  
William H Fishman
Keyword(s):  
Quality Control ◽  
Alkaline Phosphatase ◽  
Control Program ◽  
Total Alkaline Phosphatase ◽  
Quality Control Program ◽  
Total Alkaline Phosphatase Activity ◽  
Standard Curve ◽  
Heat Stable ◽  
One Year ◽  
Total Alkaline

Abstract A quality-control program, in which a heat-stable human alkaline phosphatase preparation is used to monitor the performance of an automated alkaline phosphatase method, was observed for a year. In the method phenyl phosphate is used as substrate, L-phenylalanine as an intestinal isoenzyme inhibitor, and a phenol standard curve to monitor chromogen formation. Mean total alkaline phosphatase activity was 14.0 ± 0.9 King-Armstrong units (CV, 6.4%), and mean inhibition by L-phenylalanine was 78.2 ± 1.1% (CV, 1.4%). These data compared closely with the data obtained in a collaborating laboratory during the same one-year period.

Factors affecting measurement of total alkaline phosphatase activity in human serum, especially wavelength accuracy.

1978 ◽  
Vol 24 (6) ◽  
pp. 938-941 ◽  
Author(s):  
J A Lott ◽  
K Turner ◽  
J Scott
Keyword(s):  
Alkaline Phosphatase ◽  
Human Serum ◽  
Alkaline Phosphatase Activity ◽  
Serum Alkaline Phosphatase ◽  
Molar Absorptivity ◽  
Total Alkaline Phosphatase ◽  
Factors Affecting ◽  
Total Alkaline Phosphatase Activity ◽  
Setting Error ◽  
Total Alkaline

Abstract Serum alkaline phosphatase estimations by the Selected Method [Clin. Chem. 21, 1988 (1975)] are very sensitive to small errors in the wavelength setting of the spectrophotometer. With a setting error as small as 3 nm, results can be too low by as much as 23%. The product of the reaction, 4-nitrophenol, can be prepared in very pure form and can be used to check both the wavelength and absorbance accuracy of a spectrophotometer. The molar absorptivity for 4-nitrophenol obtained in the instrument being used should be used in calculating alkaline phosphatase activities.

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