scholarly journals Plasma-membrane-associated immunoglobulins and other polypeptides of pig mesenteric node lymphocytes

1975 ◽  
Vol 148 (3) ◽  
pp. 417-423 ◽  
Author(s):  
S I Chavin ◽  
S M Johnson ◽  
A Holliman
Keyword(s):  
Molecular Weight ◽  
Plasma Membrane ◽  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Plasma Membranes ◽  
Intact Cell ◽  
Immunoglobulin M ◽  
Mesenteric Lymph ◽  
Mesenteric Node ◽  
Polypeptide Chains

Plasma membranes from pig mesenteric lymph-node lymphocytes contain a large number of polypeptide chains ranging in molecular weight from 20,000 to greater than 470,000, with a major component of 46,000. There are approx. 12-15 glycoproteins. The membranes contain immunoglobulin G, which comprises 0.6% of the total protein. Immunoglobulin M is also detected, but has not been accurately quantified for technical reasons. Possible origins of the membrane-associated immunoglobulin are briefly discussed. It is concluded that the immunoglobulin is probably associated with the plasma membrane in the intact cell.

scholarly journals Carbohydrate composition of lymphocyte plasma membrane from pig mesenteric lymph node

1976 ◽  
Vol 153 (1) ◽  
pp. 75-78 ◽  
Author(s):  
D Snary ◽  
A K Allen ◽  
R A Faulkes ◽  
M J Crumpton
Keyword(s):  
Plasma Membrane ◽  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Amino Sugar ◽  
Membrane Lipid ◽  
Molar Ratio ◽  
Neutral Sugar ◽  
Mesenteric Lymph ◽  
Lipid Fractions ◽  
Almost All

Pig lymphocyte plasma membrane isolated from mesenteric lymph node contained 69 mug of carbohydrate/mg dry wt., which was made up of neutral sugar, amino sugar and sialic acid in the molar proportions 5:1.7:1. The neutral sugar comprised fucose, ribose, mannose, glucose, galactose and inositol (molar proportions 2:9:11:15:26:1), and the amino sugar glucosamine and galactosamine (molar ratio 2:1). The ribose was most probably derived from RNA. All of the fucose and mannose and almost all of the glucosamine were associated with the membrane protein whereas the membrane lipid contained all of the inositol. The remaining sugars were distributed in various ratios between the protein and lipid fractions.

scholarly journals Lipid composition of lymphocyte plasma membrane from pig mesenteric lymph node

1976 ◽  
Vol 156 (1) ◽  
pp. 103-110 ◽  
Author(s):  
G M Levis ◽  
G P Evangelatos ◽  
M J Crumpton
Keyword(s):  
Plasma Membrane ◽  
Oleic Acid ◽  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Lipid Composition ◽  
Membrane Fraction ◽  
Neutral Lipids ◽  
Lipid Fraction ◽  
Mesenteric Lymph ◽  
Major Ganglioside

1. The lipid fraction of the plasma membrane of pig mesenteric lymph-node lymphocytes contained primarily (94%) neutral lipids and phospholipids in about equal weights. The remianing lipid comprised glycosphingolipids (1.8%), gangliosides (o.27%)and probably ceramides (1.3%). The major phospholipid was phosphatidylcholine (46% of the total), and mono- and tri-hexosylceramides accounted for 72% of the glycosphingolipids. Haematoside was distributed between the glycosphingolipid and ganglioside fractions. The major ganglioside was monosialoganglioside. About 90% of the sialic acid was N-glycollylated. 2. A comparision of the lipid composition of the plasma-membrane fraction with that of the initial lymph-node homogenate showed that the purified membrane contained increased proportions of phospholipids, especially sphingomyelin, glycosphingolipids and gangliosides. 3. Fatty acid analyses showed that the membrane phosphatidylcholine was rich in palmitic acid, that the sphingomeyelin and phosphatidylethanolamine were high in myristic acid and that the glycosphingolipids were rich in oleic acid.

An estimate of Salmonella prevalence on Illinois swine farms using mesenteric lymph node cultures

1999 ◽  
Author(s):  
D. Damman ◽  
P. B. Bahnson ◽  
R. M. Weigel ◽  
R. E. Isaacson ◽  
H. F. Troutt ◽  
...  
Keyword(s):  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Swine Farms ◽  
Mesenteric Lymph

scholarly journals Rotavirus infection causes mesenteric lymph node hypertrophy independently of type I interferon or TNFa in mice

2020 ◽  
Author(s):  
Joy Nakawesi ◽  
Konjit Getachew Muleta ◽  
Dragos‐Christian Dasoveanu ◽  
Bengt Johansson‐Lindbom ◽  
Katharina Lahl
Keyword(s):  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Type I Interferon ◽  
Type I ◽  
Rotavirus Infection ◽  
Mesenteric Lymph
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