scholarly journals Carbohydrate composition of lymphocyte plasma membrane from pig mesenteric lymph node

1976 ◽  
Vol 153 (1) ◽  
pp. 75-78 ◽  
Author(s):  
D Snary ◽  
A K Allen ◽  
R A Faulkes ◽  
M J Crumpton
Keyword(s):  
Plasma Membrane ◽  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Amino Sugar ◽  
Membrane Lipid ◽  
Molar Ratio ◽  
Neutral Sugar ◽  
Mesenteric Lymph ◽  
Lipid Fractions ◽  
Almost All

Pig lymphocyte plasma membrane isolated from mesenteric lymph node contained 69 mug of carbohydrate/mg dry wt., which was made up of neutral sugar, amino sugar and sialic acid in the molar proportions 5:1.7:1. The neutral sugar comprised fucose, ribose, mannose, glucose, galactose and inositol (molar proportions 2:9:11:15:26:1), and the amino sugar glucosamine and galactosamine (molar ratio 2:1). The ribose was most probably derived from RNA. All of the fucose and mannose and almost all of the glucosamine were associated with the membrane protein whereas the membrane lipid contained all of the inositol. The remaining sugars were distributed in various ratios between the protein and lipid fractions.

scholarly journals Plasma-membrane-associated immunoglobulins and other polypeptides of pig mesenteric node lymphocytes

1975 ◽  
Vol 148 (3) ◽  
pp. 417-423 ◽  
Author(s):  
S I Chavin ◽  
S M Johnson ◽  
A Holliman
Keyword(s):  
Molecular Weight ◽  
Plasma Membrane ◽  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Plasma Membranes ◽  
Intact Cell ◽  
Immunoglobulin M ◽  
Mesenteric Lymph ◽  
Mesenteric Node ◽  
Polypeptide Chains

Plasma membranes from pig mesenteric lymph-node lymphocytes contain a large number of polypeptide chains ranging in molecular weight from 20,000 to greater than 470,000, with a major component of 46,000. There are approx. 12-15 glycoproteins. The membranes contain immunoglobulin G, which comprises 0.6% of the total protein. Immunoglobulin M is also detected, but has not been accurately quantified for technical reasons. Possible origins of the membrane-associated immunoglobulin are briefly discussed. It is concluded that the immunoglobulin is probably associated with the plasma membrane in the intact cell.

scholarly journals Lipid composition of lymphocyte plasma membrane from pig mesenteric lymph node

1976 ◽  
Vol 156 (1) ◽  
pp. 103-110 ◽  
Author(s):  
G M Levis ◽  
G P Evangelatos ◽  
M J Crumpton
Keyword(s):  
Plasma Membrane ◽  
Oleic Acid ◽  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Lipid Composition ◽  
Membrane Fraction ◽  
Neutral Lipids ◽  
Lipid Fraction ◽  
Mesenteric Lymph ◽  
Major Ganglioside

1. The lipid fraction of the plasma membrane of pig mesenteric lymph-node lymphocytes contained primarily (94%) neutral lipids and phospholipids in about equal weights. The remianing lipid comprised glycosphingolipids (1.8%), gangliosides (o.27%)and probably ceramides (1.3%). The major phospholipid was phosphatidylcholine (46% of the total), and mono- and tri-hexosylceramides accounted for 72% of the glycosphingolipids. Haematoside was distributed between the glycosphingolipid and ganglioside fractions. The major ganglioside was monosialoganglioside. About 90% of the sialic acid was N-glycollylated. 2. A comparision of the lipid composition of the plasma-membrane fraction with that of the initial lymph-node homogenate showed that the purified membrane contained increased proportions of phospholipids, especially sphingomyelin, glycosphingolipids and gangliosides. 3. Fatty acid analyses showed that the membrane phosphatidylcholine was rich in palmitic acid, that the sphingomeyelin and phosphatidylethanolamine were high in myristic acid and that the glycosphingolipids were rich in oleic acid.

scholarly journals Preparation and characterization of the plasma membrane of pig lymphocytes

1970 ◽  
Vol 120 (1) ◽  
pp. 133-143 ◽  
Author(s):  
D. Allan ◽  
M. J. Crumpton
Keyword(s):  
Plasma Membrane ◽  
Lymph Node ◽  
Membrane Fraction ◽  
Membrane Vesicles ◽  
Sucrose Density Gradient ◽  
Molar Ratio ◽  
Whole Cells ◽  
Mesenteric Lymph ◽  
Specific Activities

Lymphocyte plasma membrane was isolated from minced pig mesenteric lymph node by differential centrifugation and by centrifuging through a sucrose density gradient. The yield of membrane was approx. 0.1% (dry wt. relative to wet wt. of lymph node). The purified material had a sucrose density of 1.14g/cm3 and consisted mainly of smooth vesicles. The membrane fraction contained, apart from protein and lipid, 59μg of carbohydrate, 11μg of sialic acid and 28μg of RNA/mg of protein; no DNA was detected. The cholesterol/phospholipid molar ratio was 1.01. Specific activities (μmol of product/h per mg of protein) of 5′-nucleotidase, succinate dehydrogenase, acid phosphatase and glucose 6-phosphatase were 10.1, 0, 0.51 and 0.30 respectively. The membrane vesicles were aggregated by an antiserum against pig lymphocytes and adsorbed the agglutinins to whole lymphocytes present in the antiserum; the membrane fraction was 28 times as effective as whole cells (on a dry wt. basis) in removing the lympho-agglutinins. Antisera against the membrane fraction agglutinated whole lymphocytes. It is concluded that the preparation represents the plasma membrane of small lymphocytes. The plasma membrane of pig thymocytes was isolated by using the same procedure. Its properties were similar to those of the lymphocyte plasma membrane.

An estimate of Salmonella prevalence on Illinois swine farms using mesenteric lymph node cultures

1999 ◽  
Author(s):  
D. Damman ◽  
P. B. Bahnson ◽  
R. M. Weigel ◽  
R. E. Isaacson ◽  
H. F. Troutt ◽  
...  
Keyword(s):  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Swine Farms ◽  
Mesenteric Lymph

scholarly journals Rotavirus infection causes mesenteric lymph node hypertrophy independently of type I interferon or TNFa in mice

2020 ◽  
Author(s):  
Joy Nakawesi ◽  
Konjit Getachew Muleta ◽  
Dragos‐Christian Dasoveanu ◽  
Bengt Johansson‐Lindbom ◽  
Katharina Lahl
Keyword(s):  
Lymph Node ◽  
Mesenteric Lymph Node ◽  
Type I Interferon ◽  
Type I ◽  
Rotavirus Infection ◽  
Mesenteric Lymph
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