scholarly journals Effect of an exotoxin from Bacillus thuringiensis on Deoxyribonucleic acid-dependent ribonucleic acid polymerase in nuclei from adult Sarcophaga bullata. Unusual behaviour of eukaryotic polymerases to inhibitors

1973 ◽  
Vol 136 (1) ◽  
pp. 9-13 ◽  
Author(s):  
T. J. C. Beebee ◽  
R. P. M. Bond
Keyword(s):  
Ionic Strength ◽  
Bacillus Thuringiensis ◽  
Rna Polymerase ◽  
Metal Ions ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Low Concentration ◽  
Sarcophaga Bullata ◽  
Unusual Behaviour

The DNA-dependent RNA polymerase activities in nuclei isolated from adult Sarcophaga bullata are unusual in their responses to metal ions, ionic strength and inhibitors. There is an activity that is sensitive both to rifamycin and to α-amanitin. The activity is less sensitive to Bacillus thuringiensis exotoxin than is larval polymerase, and low concentration of exotoxin provoke a slight stimulation.

scholarly journals Deoxyribonucleic acid-dependent ribonucleic acid polymerases from normal and polyoma-transformed BHK-21/C13 cells

1975 ◽  
Vol 145 (3) ◽  
pp. 509-516 ◽  
Author(s):  
R J Cooper ◽  
H M Keir
Keyword(s):  
Ionic Strength ◽  
Rna Polymerase ◽  
Dna Content ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Actinomycin D ◽  
Activity Ratio ◽  
Cell Types ◽  
Dna Templates ◽  
Alpha Amanitin

DNA-dependent RNA polymerase (EC 2.7.7.6) ACTIVITIES FROM NORMAL BHK-21/C13 cells and from BHK-21/C13 cells transformed by polyoma virus (PYY cells) were solubilized and fractionated on columns of DEAE-Sephadex. Various properties of the A and B enzymes from the two types of cell were compared. 1. The yields of polymerase relative to the DNA content of the nuclear preparations are similar for both cell types. 2. The ionic-strength optima of polymerases A and B are 12.5 mM and 100mM with respect to (NH4)2SO4 for both cell types. 3. The Mn2+/Mg2+ activity ratio (measured at the respective optimum for each cation) for polymerase A from BHK-21/C13 cells was 1.48 and for the polymerase A from PYY cells was 0.55. The corresponding ratios for polymerase B were 10.11 for BHK-21/C13 cells and 22.75 for PYY cells. 4. Minor differences in the ability of the A polymerases to transcribe native and denatured DNA templates were observed; such differences were not apparent when the B polymerases were compared. 5. All the polymerases were inhibited completely by actinomycin D and by rifampicin AF/013, but not markedly so by rifampicin. Alpha-amanitin inhibited polymerase B but not polymerase A.

scholarly journals Differential inhibition of mammalian ribonucleic acid polymerases by an exotoxin from Bacillus thuringiensis. The direct observation of nucleoplasmic ribonucleic acid polymerase activity in intact nuclei

1972 ◽  
Vol 127 (4) ◽  
pp. 619-624 ◽  
Author(s):  
T. Beebee ◽  
A. Korner ◽  
R. P. M. Bond
Keyword(s):  
Ionic Strength ◽  
Bacillus Thuringiensis ◽  
Rna Polymerase ◽  
Ribonucleic Acid ◽  
Polymerase Activity ◽  
High Ionic Strength ◽  
Rna Polymerases ◽  
Differential Inhibition ◽  
Rna Polymerase Activity ◽  
Soluble Enzymes

The effects of the exotoxin from Bacillus thuringiensis on DNA-dependent RNA polymerases from rat liver were examined. The exotoxin inhibits all RNA polymerase activity at both low and high ionic strength in intact nuclei, and soluble enzymes are similarly affected. This inhibition is relieved by ATP. Dephosphorylated exotoxin did not inhibit the soluble enzymes. Nucleolar and nucleoplasmic RNA polymerases respond to different concentration ranges of exotoxin, and the compound can be used in intact nuclei to isolate the nucleoplasmic activity.

scholarly journals A procedure for the rapid purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase (Short Communication)

1973 ◽  
Vol 133 (1) ◽  
pp. 201-203 ◽  
Author(s):  
Peter Humphries ◽  
David J. McConnell ◽  
Robert L. Gordon
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Short Communication ◽  
Gel Filtration ◽  
High Salt ◽  
Rapid Procedure ◽  
Complete Purification ◽  
Rapid Purification

A rapid procedure involving DNA–cellulose chromatography followed either by sedimentation in a high-salt glycerol gradient or by gel filtration is described for the complete purification of Escherichia coli DNA-dependent RNA polymerase.

scholarly journals Effects of α-amanitin on the stimulation of prostatic ribonucleic acid polymerase by prostatic steroid–protein receptor complexes

1974 ◽  
Vol 140 (3) ◽  
pp. 565-567 ◽  
Author(s):  
P. Davies ◽  
K. Griffiths
Keyword(s):  
Ionic Strength ◽  
Rna Polymerase ◽  
Ribonucleic Acid ◽  
Selective Inhibitor ◽  
High Ionic Strength ◽  
Receptor Complexes ◽  
Protein Receptor ◽  
Stimulation Of

Stimulation of prostatic RNA polymerase in vitro by prostatic 17β-hydroxy-5α-androstan-3-one (5α-dihydrotestosterone)–receptor complexes has been previously reported. By use of the selective inhibitor, α-amanitin, we have shown that both nucleolar and extranucleolar RNA polymerase activities may be stimulated, but stimulation is abolished at high ionic strength.

scholarly journals Effect of the exotoxin of Bacillus thuringiensis on normal and ecdysone-stimulated ribonucleic acid polymerase activity in intact nuclei from the fat body of Sarcophaga bullata larvae

1973 ◽  
Vol 136 (1) ◽  
pp. 1-7 ◽  
Author(s):  
T. J. C. Beebee ◽  
R. P. M. Bond
Keyword(s):  
Bacillus Thuringiensis ◽  
Ribonucleic Acid ◽  
Fat Body ◽  
Polymerase Activity ◽  
Sarcophaga Bullata ◽  
Steady Rate

A nuclear preparation from the fat-body of Sarcophaga bullata was obtained which incorporates nucleotides at a steady rate. Two activities, differing in their response to α-amanitin, are present. The activities are not separated by changes in the concentration of (NH4)2SO4 as effectively as in mammalian nuclei. The activity resistant to α-amanitin is stimulated by ecdysone, and both normal and the ecdysone-stimulated activities are inhibited by the exotoxin. The amanitin-sensitive enzyme is also inhibited by exotoxin, but higher concentrations are required.

scholarly journals Contemporaneous isolation of deoxyribonucleic acid-dependent ribonucleic acid polymerase and poly(A) polymerase from rat liver mitochondria

1976 ◽  
Vol 157 (2) ◽  
pp. 295-300 ◽  
Author(s):  
R Gallerani ◽  
di Istituto ◽  
Ch Istituto di ◽  
C Saccone
Keyword(s):  
Rna Polymerase ◽  
Rat Liver ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Chromatographic Behaviour ◽  
Endogenous Factor ◽  
Insoluble Material ◽  
Protein Molecules

1. Poly(A) polymerase and DNA-dependent RNA polymerase from rat liver mitochondria can be completely separated by using two different chromatographic procedures. 2. Poly(A) polymerase can only incorporate ATP into acid-insoluble material and strongly depends on the addition of an endogenous factor (probably containing a mixture of oligoribonucleotides), but it is not stimulated by DNA. 3. RNA polymerase is fully DNA-dependent and rifampicin-sensitive, but was not stimulated by the endogenous factor mentioned above. 4. The chromatographic behaviour of the two enzymes, together with the properties described, suggest that they represent two different protein molecules.

scholarly journals The early enhancement of rat liver deoxyribonucleic acid-dependent ribonucleic acid polymerase II activity by tri-iodothyronine

1975 ◽  
Vol 150 (1) ◽  
pp. 133-135 ◽  
Author(s):  
S Jothy ◽  
J L Bilodeau ◽  
H Champsaur ◽  
H Simpkins
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Rat Liver ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Hormonal Treatment ◽  
Simultaneous Increase ◽  
Transient Activation ◽  
Acidic Proteins

It is shown that tri-iodothyronine injected intravenously into thyroidectomized rats induces an early and transient activation of rat liver RNA polymerase II which could be demonstrated to occur 40-80 min after hormonal treatment. There was a simultaneous increase in the concentration of acidic proteins bound to chromatin.

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