scholarly journals The early enhancement of rat liver deoxyribonucleic acid-dependent ribonucleic acid polymerase II activity by tri-iodothyronine

1975 ◽  
Vol 150 (1) ◽  
pp. 133-135 ◽  
Author(s):  
S Jothy ◽  
J L Bilodeau ◽  
H Champsaur ◽  
H Simpkins
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Rat Liver ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Hormonal Treatment ◽  
Simultaneous Increase ◽  
Transient Activation ◽  
Acidic Proteins

It is shown that tri-iodothyronine injected intravenously into thyroidectomized rats induces an early and transient activation of rat liver RNA polymerase II which could be demonstrated to occur 40-80 min after hormonal treatment. There was a simultaneous increase in the concentration of acidic proteins bound to chromatin.

scholarly journals Contemporaneous isolation of deoxyribonucleic acid-dependent ribonucleic acid polymerase and poly(A) polymerase from rat liver mitochondria

1976 ◽  
Vol 157 (2) ◽  
pp. 295-300 ◽  
Author(s):  
R Gallerani ◽  
di Istituto ◽  
Ch Istituto di ◽  
C Saccone
Keyword(s):  
Rna Polymerase ◽  
Rat Liver ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Chromatographic Behaviour ◽  
Endogenous Factor ◽  
Insoluble Material ◽  
Protein Molecules

1. Poly(A) polymerase and DNA-dependent RNA polymerase from rat liver mitochondria can be completely separated by using two different chromatographic procedures. 2. Poly(A) polymerase can only incorporate ATP into acid-insoluble material and strongly depends on the addition of an endogenous factor (probably containing a mixture of oligoribonucleotides), but it is not stimulated by DNA. 3. RNA polymerase is fully DNA-dependent and rifampicin-sensitive, but was not stimulated by the endogenous factor mentioned above. 4. The chromatographic behaviour of the two enzymes, together with the properties described, suggest that they represent two different protein molecules.

scholarly journals A procedure for the rapid purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase (Short Communication)

1973 ◽  
Vol 133 (1) ◽  
pp. 201-203 ◽  
Author(s):  
Peter Humphries ◽  
David J. McConnell ◽  
Robert L. Gordon
Keyword(s):  
Escherichia Coli ◽  
Rna Polymerase ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Short Communication ◽  
Gel Filtration ◽  
High Salt ◽  
Rapid Procedure ◽  
Complete Purification ◽  
Rapid Purification

A rapid procedure involving DNA–cellulose chromatography followed either by sedimentation in a high-salt glycerol gradient or by gel filtration is described for the complete purification of Escherichia coli DNA-dependent RNA polymerase.

scholarly journals Inhibition by α-amanitin of ribonucleic acid polymerase solubilized from rat liver nuclei

1970 ◽  
Vol 116 (2) ◽  
pp. 177-180 ◽  
Author(s):  
F. Novello ◽  
L. Fiume ◽  
F. Stirpe
Keyword(s):  
Rna Polymerase ◽  
Ammonium Sulphate ◽  
Rat Liver ◽  
Ribonucleic Acid ◽  
Isolated Rat Liver ◽  
Rat Liver Nuclei ◽  
Liver Nuclei ◽  
Isolated Rat

1. α-Amanitin inhibits in vitro the RNA polymerase solubilized from isolated rat liver nuclei. 2. In contrast with previous observations with whole nuclei, the inhibition occurs approximately to the same extent in the presence and in the absence of ammonium sulphate. 3. Evidence is presented that the toxin acts by interacting with the enzyme itself and not with DNA or other components.

scholarly journals Two species of RNA polymerase II released from rat liver chromatin

FEBS Letters ◽  
1979 ◽  
Vol 104 (1) ◽  
pp. 169-172 ◽  
Author(s):  
Munehiko Yukioka ◽  
Koichiro Omori ◽  
Yasuji Okai ◽  
Akira Inoue
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Rat Liver ◽  
Liver Chromatin
Sign in / Sign up

Export Citation Format

Share Document