scholarly journals Effect of the exotoxin of Bacillus thuringiensis on normal and ecdysone-stimulated ribonucleic acid polymerase activity in intact nuclei from the fat body of Sarcophaga bullata larvae

1973 ◽  
Vol 136 (1) ◽  
pp. 1-7 ◽  
Author(s):  
T. J. C. Beebee ◽  
R. P. M. Bond
Keyword(s):  
Bacillus Thuringiensis ◽  
Ribonucleic Acid ◽  
Fat Body ◽  
Polymerase Activity ◽  
Sarcophaga Bullata ◽  
Steady Rate

A nuclear preparation from the fat-body of Sarcophaga bullata was obtained which incorporates nucleotides at a steady rate. Two activities, differing in their response to α-amanitin, are present. The activities are not separated by changes in the concentration of (NH4)2SO4 as effectively as in mammalian nuclei. The activity resistant to α-amanitin is stimulated by ecdysone, and both normal and the ecdysone-stimulated activities are inhibited by the exotoxin. The amanitin-sensitive enzyme is also inhibited by exotoxin, but higher concentrations are required.

scholarly journals Effect of an exotoxin from Bacillus thuringiensis on Deoxyribonucleic acid-dependent ribonucleic acid polymerase in nuclei from adult Sarcophaga bullata. Unusual behaviour of eukaryotic polymerases to inhibitors

1973 ◽  
Vol 136 (1) ◽  
pp. 9-13 ◽  
Author(s):  
T. J. C. Beebee ◽  
R. P. M. Bond
Keyword(s):  
Ionic Strength ◽  
Bacillus Thuringiensis ◽  
Rna Polymerase ◽  
Metal Ions ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Low Concentration ◽  
Sarcophaga Bullata ◽  
Unusual Behaviour

The DNA-dependent RNA polymerase activities in nuclei isolated from adult Sarcophaga bullata are unusual in their responses to metal ions, ionic strength and inhibitors. There is an activity that is sensitive both to rifamycin and to α-amanitin. The activity is less sensitive to Bacillus thuringiensis exotoxin than is larval polymerase, and low concentration of exotoxin provoke a slight stimulation.

scholarly journals Differential inhibition of mammalian ribonucleic acid polymerases by an exotoxin from Bacillus thuringiensis. The direct observation of nucleoplasmic ribonucleic acid polymerase activity in intact nuclei

1972 ◽  
Vol 127 (4) ◽  
pp. 619-624 ◽  
Author(s):  
T. Beebee ◽  
A. Korner ◽  
R. P. M. Bond
Keyword(s):  
Ionic Strength ◽  
Bacillus Thuringiensis ◽  
Rna Polymerase ◽  
Ribonucleic Acid ◽  
Polymerase Activity ◽  
High Ionic Strength ◽  
Rna Polymerases ◽  
Differential Inhibition ◽  
Rna Polymerase Activity ◽  
Soluble Enzymes

The effects of the exotoxin from Bacillus thuringiensis on DNA-dependent RNA polymerases from rat liver were examined. The exotoxin inhibits all RNA polymerase activity at both low and high ionic strength in intact nuclei, and soluble enzymes are similarly affected. This inhibition is relieved by ATP. Dephosphorylated exotoxin did not inhibit the soluble enzymes. Nucleolar and nucleoplasmic RNA polymerases respond to different concentration ranges of exotoxin, and the compound can be used in intact nuclei to isolate the nucleoplasmic activity.

Characterization of Bacillus thuringiensis Cry toxin binding novel GPI anchored aminopeptidase from fat body of the moth Spodoptera litura

2007 ◽  
Vol 29 (11) ◽  
pp. 1651-1657 ◽  
Author(s):  
Madhusudhan Budatha ◽  
Gargi Meur ◽  
P. B. Kirti ◽  
Aparna Dutta Gupta
Keyword(s):  
Bacillus Thuringiensis ◽  
Spodoptera Litura ◽  
Fat Body ◽  
Toxin Binding ◽  
Cry Toxin

scholarly journals Ribonucleic acid polymerase activities in Jerusalem-artichoke tissue

1974 ◽  
Vol 143 (1) ◽  
pp. 107-113 ◽  
Author(s):  
John R. Gore ◽  
John Ingle
Keyword(s):  
Rna Polymerase ◽  
Ribonucleic Acid ◽  
Deae Cellulose ◽  
Jerusalem Artichoke ◽  
Polymerase Activity ◽  
Tuber Tissue ◽  
Rna Polymerase Activity ◽  
Rna Accumulation ◽  
The Relationship

1. Artichoke tuber tissue contained RNA polymerase activity bound to the chromatin and in the supernatant after chromatin sedimentation. 2. The activity in the supernatant, the soluble polymerase, was fractionated into polymerases I and II by DEAE-cellulose chromatography, and the properties of each activity were determined. 3. The proportions of chromatin-bound and soluble activities varied with growth of the tissue, and there was a correlation between chromatin-bound activity and RNA accumulation. 4. The properties of the solubilized chromatin activity were compared with those of the soluble activity, and the relationship between these two activities is discussed.

scholarly journals Activation of MAP kinase pathways in Galleria mellonella infected with Bacillus thuringiensis.

2014 ◽  
Vol 61 (1) ◽  
Author(s):  
Iwona Wojda ◽  
Konrad Koperwas ◽  
Teresa Jakubowicz
Keyword(s):  
Heat Shock ◽  
Bacillus Thuringiensis ◽  
Map Kinase ◽  
Map Kinases ◽  
Galleria Mellonella ◽  
Fat Body ◽  
Greater Wax Moth ◽  
Fat Bodies ◽  
Map Kinase Pathways ◽  
Wax Moth

We followed changes in the level of phospho-MAP kinases in the greater wax moth Galleria mellonella after infection with Bacillus thuringiensis. We observed an enhanced level of phosphorylated p38 and JNK in fat bodies of the infected larvae. In hemocytes, injection of B. thuringiensis caused the highest increase in phospho-JNK, however, all pathways were activated after aseptic injection. We report that Galleria mellonella larvae exposed to heat shock before infection showed an enhanced level of phosphorylated JNK in fat body. This finding is relevant in the light of our previous reports, which submit evidence that pre-shocked animals are more resistant to infection.

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