scholarly journals Self-renaturing fractions in the separated strands of mouse satellite deoxyribonucleic acid

1972 ◽  
Vol 128 (2) ◽  
pp. 193-198 ◽  
Author(s):  
W. D. Sutton ◽  
P. M. B. Walker
Keyword(s):  
Satellite Dna ◽  
Deoxyribonucleic Acid ◽  
Gradient Centrifugation ◽  
High Thermal Stability ◽  
The Self ◽  
The Other ◽  
Cscl Gradient ◽  
Dna Strands ◽  
Variable Extent ◽  
Mouse Satellite Dna

Pyrimidine- and purine-rich strands of Mus musculus satellite DNA prepared by alkaline CsCl-gradient centrifugation can self-renature to a variable extent to give partial duplexes with high thermal stability. These duplexes were purified by treatment with nuclease S1 followed by hydroxylapatite chromatography, and have been shown by pyrimidine-tract analysis to be very similar in sequence to total reassociated satellite DNA. It is believed that the self-renaturing fractions result from variable contamination of each strand with fragments of the other, rather than from molecular inversions. The predominantly single-stranded properties of these fractions may be partly due to the ability of mouse satellite DNA strands to reassociate in non-stoicheiometric proportions.

Satellite deoxyribonucleic acid in Pseudomonas aeruginosa

1972 ◽  
Vol 18 (7) ◽  
pp. 1003-1006
Author(s):  
Ruth B. Finley ◽  
J. D. Punch
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Satellite Dna ◽  
Deoxyribonucleic Acid ◽  
The Other ◽  
Clinical Specimens

Deoxyribonucleic acid preparations from two strains of Pseudomonas aeruginosa newly isolated from clinical specimens each contained double-stranded satellite DNA. The satellite DNA in one strain consisted of two components with buoyant densities of 1.705 and 1.712 g cm−3 and comprised 28% of the total extracted DNA. The other strain contained 15% satellite DNA which was composed of components with buoyant densities of 1.711 and 1.718 g cm−3.

scholarly journals Acquisition of hyaluronate-binding affinity in vivo by newly synthesized cartilage proteoglycans

1989 ◽  
Vol 258 (3) ◽  
pp. 875-880 ◽  
Author(s):  
J D Sandy ◽  
J R O'Neill ◽  
L C Ratzlaff
Keyword(s):  
Binding Affinity ◽  
Gradient Centrifugation ◽  
The Other ◽  
Binding Properties ◽  
High Affinity ◽  
Cscl Gradient ◽  
Cartilage Proteoglycans ◽  
Relationship Of ◽  
Total Tissue

We have studied the hyaluronate-binding properties of aggregating cartilage proteoglycans synthesized in vivo by immature (6-week), mature (25-week) and aged (75-week) rabbits. Precursor isotope (35SO4) was given by intra-articular injection and articular cartilage was removed from rabbits after periods ranging from 1.5 h to 168 h. Proteoglycans were extracted with 4 M-guanidinium/HCl and monomers were isolated by CsCl gradient centrifugation under dissociative conditions. The percentages of both radiolabelled and total tissue monomers with a high affinity for hyaluronate [that is, capable of forming aggregates on Sepharose CL-2B in the presence of 0.8% (w/w) hyaluronate] were then determined. For all samples about 30% of the tissue monomers were high-affinity; however, less than 5% of the radiolabelled monomers were high-affinity at 1.5 h after injection, and this figure increased gradually with time in vivo. The increase was rapid in immature rabbits, such that after 24 h, about 30% of the radiolabelled monomers were high-affinity; on the other hand for mature and aged rabbits the increase was markedly slower such that 30% high-affinity was attained only after about 72 h. The results show that aggregating cartilage proteoglycans are secreted in vivo in a ‘precursor’ form with a low affinity for hyaluronate, and suggest that conversion of these monomers to a form with a higher binding affinity occurs with a half-time of about 12 h in immature cartilages but greater than 24 h in mature cartilages. The possible relationship of these findings to the process of proteoglycan aggregation in vivo is discussed.

scholarly journals The complexity of satellite deoxyribonucleic acid in a higher plant

1975 ◽  
Vol 149 (1) ◽  
pp. 31-38 ◽  
Author(s):  
J Sinclair ◽  
R Wells ◽  
B Deumling ◽  
J Ingle
Keyword(s):  
Satellite Dna ◽  
Cucumis Melo ◽  
Thermal Denaturation ◽  
Deoxyribonucleic Acid ◽  
Sequence Divergence ◽  
Higher Plant ◽  
Mouse Satellite Dna

Purified satellite DNA from melon (Cucumis melo) was shown to contain at least two components from thermal-denaturation and renaturation studies. Two components were separated after partial renaturation, a fast-renaturing fraction similar in complexity to mouse satellite DNA, and one with 6000 times greater complexity. Both components renatured very accurately, indicating a minimum of sequence divergence. Centrifugation of the purified satellite DNA in Ag+/Cs2SO4 gradients resolved two major and several minor fractions. The two major fractions were only slightly enriched for fast- or slow-renaturing sequences.

Providing Freshmen with a Good “Starting-Block”

2019 ◽  
Vol 78 (1-2) ◽  
pp. 69-75 ◽  
Author(s):  
Mikaël De Clercq ◽  
Charlotte Michel ◽  
Sophie Remy ◽  
Benoît Galand
Keyword(s):  
The Self ◽  
The Other ◽  
Student Adjustment ◽  
Social Psychological ◽  
Pivotal Phase ◽  
Study Program ◽  
Study Task ◽  
The Social ◽  
Social Belonging ◽  
Intention To Persist

Abstract. Grounded in social-psychological literature, this experimental study assessed the effects of two so-called “wise” interventions implemented in a student study program. The interventions took place during the very first week at university, a presumed pivotal phase of transition. A group of 375 freshmen in psychology were randomly assigned to three conditions: control, social belonging, and self-affirmation. Following the intervention, students in the social-belonging condition expressed less social apprehension, a higher social integration, and a stronger intention to persist one month later than the other participants. They also relied more on peers as a source of support when confronted with a study task. Students in the self-affirmation condition felt more self-affirmed at the end of the intervention but didn’t benefit from other lasting effects. The results suggest that some well-timed and well-targeted “wise” interventions could provide lasting positive consequences for student adjustment. The respective merits of social-belonging and self-affirmation interventions are also discussed.

Stories and the Self

2020 ◽  
Vol 32 (2) ◽  
pp. 47-58 ◽  
Author(s):  
Stefan Krause ◽  
Markus Appel
Keyword(s):  
Meta Analysis ◽  
The Self ◽  
The Other ◽  
Lower Degree ◽  
Contrast Effects ◽  
Self Perception ◽  
Self Perceptions ◽  
Other Hand ◽  
The One ◽  
Implicit And Explicit

Abstract. Two experiments examined the influence of stories on recipients’ self-perceptions. Extending prior theory and research, our focus was on assimilation effects (i.e., changes in self-perception in line with a protagonist’s traits) as well as on contrast effects (i.e., changes in self-perception in contrast to a protagonist’s traits). In Experiment 1 ( N = 113), implicit and explicit conscientiousness were assessed after participants read a story about either a diligent or a negligent student. Moderation analyses showed that highly transported participants and participants with lower counterarguing scores assimilate the depicted traits of a story protagonist, as indicated by explicit, self-reported conscientiousness ratings. Participants, who were more critical toward a story (i.e., higher counterarguing) and with a lower degree of transportation, showed contrast effects. In Experiment 2 ( N = 103), we manipulated transportation and counterarguing, but we could not identify an effect on participants’ self-ascribed level of conscientiousness. A mini meta-analysis across both experiments revealed significant positive overall associations between transportation and counterarguing on the one hand and story-consistent self-reported conscientiousness on the other hand.

Don’t Put Me in This Group

2019 ◽  
Vol 50 (2) ◽  
pp. 80-93
Author(s):  
Jort de Vreeze ◽  
Christina Matschke
Keyword(s):  
Positive Emotions ◽  
Negative Emotions ◽  
Negative Information ◽  
The Self ◽  
The Other ◽  
Other Hand ◽  
Group Memberships

Abstract. Not all group memberships are self-chosen. The current research examines whether assignments to non-preferred groups influence our relationship with the group and our preference for information about the ingroup. It was expected and found that, when people are assigned to non-preferred groups, they perceive the group as different to the self, experience negative emotions about the assignment and in turn disidentify with the group. On the other hand, when people are assigned to preferred groups, they perceive the group as similar to the self, experience positive emotions about the assignment and in turn identify with the group. Finally, disidentification increases a preference for negative information about the ingroup.

From Container to Claustrum: Projective Identification in Couples

2014 ◽  
Vol 4 (2) ◽  
Author(s):  
Tamara Feldman
Keyword(s):  
Psychological Health ◽  
Projective Identification ◽  
The Self ◽  
The Other ◽  
Intimate Partners ◽  
And Control ◽  
The Relationship ◽  
As If

This paper is a contribution to the growing literature on the role of projective identification in understanding couples' dynamics. Projective identification as a defence is well suited to couples, as intimate partners provide an ideal location to deposit unwanted parts of the self. This paper illustrates how projective identification functions differently depending on the psychological health of the couple. It elucidates how healthier couples use projective identification more as a form of communication, whereas disturbed couples are inclined to employ it to invade and control the other, as captured by Meltzer's concept of "intrusive identification". These different uses of projective identification affect couples' capacities to provide what Bion called "containment". In disturbed couples, partners serve as what Meltzer termed "claustrums" whereby projections are not contained, but imprisoned or entombed in the other. Applying the concept of claustrum helps illuminate common feelings these couples express, such as feeling suffocated, stifled, trapped, held hostage, or feeling as if the relationship is killing them. Finally, this paper presents treatment challenges in working with more disturbed couples.

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