scholarly journals The complexity of satellite deoxyribonucleic acid in a higher plant

1975 ◽  
Vol 149 (1) ◽  
pp. 31-38 ◽  
Author(s):  
J Sinclair ◽  
R Wells ◽  
B Deumling ◽  
J Ingle
Keyword(s):  
Satellite Dna ◽  
Cucumis Melo ◽  
Thermal Denaturation ◽  
Deoxyribonucleic Acid ◽  
Sequence Divergence ◽  
Higher Plant ◽  
Mouse Satellite Dna

Purified satellite DNA from melon (Cucumis melo) was shown to contain at least two components from thermal-denaturation and renaturation studies. Two components were separated after partial renaturation, a fast-renaturing fraction similar in complexity to mouse satellite DNA, and one with 6000 times greater complexity. Both components renatured very accurately, indicating a minimum of sequence divergence. Centrifugation of the purified satellite DNA in Ag+/Cs2SO4 gradients resolved two major and several minor fractions. The two major fractions were only slightly enriched for fast- or slow-renaturing sequences.

scholarly journals Self-renaturing fractions in the separated strands of mouse satellite deoxyribonucleic acid

1972 ◽  
Vol 128 (2) ◽  
pp. 193-198 ◽  
Author(s):  
W. D. Sutton ◽  
P. M. B. Walker
Keyword(s):  
Satellite Dna ◽  
Deoxyribonucleic Acid ◽  
Gradient Centrifugation ◽  
High Thermal Stability ◽  
The Self ◽  
The Other ◽  
Cscl Gradient ◽  
Dna Strands ◽  
Variable Extent ◽  
Mouse Satellite Dna

Pyrimidine- and purine-rich strands of Mus musculus satellite DNA prepared by alkaline CsCl-gradient centrifugation can self-renature to a variable extent to give partial duplexes with high thermal stability. These duplexes were purified by treatment with nuclease S1 followed by hydroxylapatite chromatography, and have been shown by pyrimidine-tract analysis to be very similar in sequence to total reassociated satellite DNA. It is believed that the self-renaturing fractions result from variable contamination of each strand with fragments of the other, rather than from molecular inversions. The predominantly single-stranded properties of these fractions may be partly due to the ability of mouse satellite DNA strands to reassociate in non-stoicheiometric proportions.

Mesophilic mutants of an obligate psychrophile, Micrococcus cryophilus

1969 ◽  
Vol 15 (10) ◽  
pp. 1145-1150 ◽  
Author(s):  
P-C. Tai ◽  
H. Jackson
Keyword(s):  
Growth Temperature ◽  
Metabolic Pathways ◽  
Thermal Denaturation ◽  
Deoxyribonucleic Acid ◽  
Elevated Temperatures ◽  
Maximum Growth ◽  
Maximal Growth ◽  
Denaturation Temperature ◽  
Maximum Growth Temperature ◽  
Thermal Denaturation Temperature

Several mutants with elevated maximal growth temperature (MGT) were developed from an obligate psychrophile, Micrococcus cryophilus ATCC 15174, by ultraviolet irradiation. Two of the mutants, T8 and M19, have the most similar characteristics to those of their parent. The mutants lost the ability to grow well at 0 °C and showed changes in metabolic pathways while acquiring the ability to grow at elevated temperatures. Heat resistance and deoxyribonucleic acid thermal denaturation temperature were shown to be unrelated to maximum growth temperature. The significance of the mutants is discussed.

scholarly journals KINETOPLAST DEOXYRIBONUCLEIC ACID OF THE HEMOFLAGELLATE TRYPANOSOMA LEWISI

1970 ◽  
Vol 47 (3) ◽  
pp. 689-702 ◽  
Author(s):  
Hartmut C. Renger ◽  
David R. Wolstenholme
Keyword(s):  
Electron Microscope ◽  
Ethidium Bromide ◽  
Thermal Denaturation ◽  
Deoxyribonucleic Acid ◽  
Cesium Chloride ◽  
Kinetoplast Dna ◽  
Microscope Examination ◽  
Main Band ◽  
Trypanosoma Lewisi ◽  
High Degree

Cesium chloride centrifugation of DNA extracted from cells of blood strain Trypanosoma lewisi revealed a main band, ρ = 1.707, a light satellite, ρ = 1.699, and a heavy satellite, ρ = 1.721. Culture strain T. lewisi DNA comprised only a main band, ρ = 1.711, and a light satellite, ρ = 1.699. DNA isolated from DNase-treated kinetoplast fractions of both the blood and culture strains consisted of only the light satellite DNA. Electron microscope examination of rotary shadowed preparations of lysates revealed that DNA from kinetoplast fractions was mainly in the form of single 0.4 µ circular molecules and large masses of 0.4 µ interlocked circles with which longer, often noncircular molecules were associated. The 0.4 µ circular molecules were mainly in the covalently closed form: they showed a high degree of resistance to thermal denaturation which was lost following sonication; and they banded at a greater density than linear DNA in cesium chloride-ethidium bromide gradients. Interpretation of the large masses of DNA as comprising interlocked covalently closed 0.4 µ circles was supported by the findings that they banded with single circular molecules in cesium chloride-ethidium bromide gradients, and following breakage of some circles by mild sonication, they disappeared and were replaced by molecules made up of low numbers of apparently interlocked 0.4 µ circles. When culture strain cells were grown in the presence of either ethidium bromide or acriflavin, there was a loss of stainable kinetoplast DNA in cytological preparations. There was a parallel loss of light satellite and of circular molecules from DNA extracted from these cells.

Molecular Hybridization of Mouse Satellite DNA-Complementary RNA in Ultrathin Sections Prepared for Electron Microscopy

1974 ◽  
Vol 14 (2) ◽  
pp. 253-261
Author(s):  
J. JACOB ◽  
KATHERINE GILLIES ◽  
D. MACLEOD ◽  
K. W. JONES
Keyword(s):  
Electron Microscopy ◽  
Satellite Dna ◽  
Similar Distribution ◽  
Tissue Sections ◽  
Satellite Sequences ◽  
Interphase Cells ◽  
Nucleolar Chromatin ◽  
Ultrathin Sections ◽  
Mouse Satellite Dna

The feasibility of in situ hybridization in tissue sections prepared for electron microscopy has been examined using mouse satellite DNA-complementary RNA and mouse L cells. The results obtained are encouraging, although certain technical aspects require further clarification. In interphase cells, hybrid-forming sites occur in chromatin patches positioned along the nuclear envelope. It is also confirmed that satellite DNA occurs in nucleolus-associated chromatin. The results suggest that satellite sequences are present in intranucleolar and peri-nucleolar chromatin. A similar distribution is indicated for ribosomal cistrons.

scholarly journals Nudeotide sequence of mouse satellite DNA

1981 ◽  
Vol 9 (3) ◽  
pp. 683-696 ◽  
Author(s):  
Wolfram Hörz ◽  
Werner Altenburger
Keyword(s):  
Satellite Dna ◽  
Mouse Satellite Dna

The organization of the mouse satellite DNA at centromeres

1989 ◽  
Vol 183 (2) ◽  
pp. 494-500 ◽  
Author(s):  
Ann Joseph ◽  
A.R. Mitchell ◽  
O.J. Miller
Keyword(s):  
Satellite Dna ◽  
Mouse Satellite Dna

scholarly journals Microtubule-associated protein MAP2 preferentially binds to a dA/dT sequence present in mouse satellite DNA.

1983 ◽  
Vol 2 (8) ◽  
pp. 1229-1234 ◽  
Author(s):  
J. Avila ◽  
E. Montejo de Garcini ◽  
F. Wandosell ◽  
A. Villasante ◽  
J.M. Sogo ◽  
...  
Keyword(s):  
Satellite Dna ◽  
Mouse Satellite Dna

scholarly journals Mouse Satellite DNA

Nature ◽  
1967 ◽  
Vol 215 (5101) ◽  
pp. 575-575
Author(s):  
Keyword(s):  
Satellite Dna ◽  
Mouse Satellite Dna

Satellite deoxyribonucleic acid in Pseudomonas aeruginosa

1972 ◽  
Vol 18 (7) ◽  
pp. 1003-1006
Author(s):  
Ruth B. Finley ◽  
J. D. Punch
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Satellite Dna ◽  
Deoxyribonucleic Acid ◽  
The Other ◽  
Clinical Specimens

Deoxyribonucleic acid preparations from two strains of Pseudomonas aeruginosa newly isolated from clinical specimens each contained double-stranded satellite DNA. The satellite DNA in one strain consisted of two components with buoyant densities of 1.705 and 1.712 g cm−3 and comprised 28% of the total extracted DNA. The other strain contained 15% satellite DNA which was composed of components with buoyant densities of 1.711 and 1.718 g cm−3.

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