scholarly journals Distribution of sodium-plus-potassium ion-stimulated adenosine triphosphatase along the length of rat small intestine and its subcellular localization

1972 ◽  
Vol 128 (1) ◽  
pp. 53P-54P
Author(s):  
M S Gnanaprakasam ◽  
L M Srivastava
1992 ◽  
Vol 282 (2) ◽  
pp. 565-570 ◽  
Author(s):  
B Ruyter ◽  
J S Lund ◽  
M S Thomassen ◽  
E N Christiansen

The subcellular localization of dihydroxyacetone phosphate acyltransferase (DHAPAT) activity in rat small intestine was investigated by Nycodenz-gradient centrifugation. We found that DHAPAT had a predominant peroxisomal distribution, with a separate enzyme activity located in the microsomal fraction, the same distribution as found in rat liver. The effect of feeding rats on a diet with 20% (w/w) partially hydrogenated fish oil (PHFO) or 0.3% clofibrate on the activity of DHAPAT in rat small intestine and liver was studied. Both 20% PHFO and 0.3% clofibrate gave a 1.8-fold stimulation of the specific activities of DHAPAT in peroxisomes of the small intestine, whereas in the liver 20% PHFO gave a 1.4-fold stimulation and 0.3% clofibrate a 1.6-fold stimulation of the total DHAPAT activities in the postnuclear supernatant. The specific activities of DHAPAT in liver were not affected.


1990 ◽  
Vol 34 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Magny S. Thomassen ◽  
Torill Rørtveit ◽  
Astrid Nilsson ◽  
Kristian Prydz ◽  
Erling N. Christiansen

2001 ◽  
Vol 120 (5) ◽  
pp. A183-A183
Author(s):  
H KOBAYASHI ◽  
H NAGATA ◽  
S MIURA ◽  
T AZUMA ◽  
H SUZUKI ◽  
...  

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