scholarly journals Invertebrate connective tissue. Isolation of d-arabinose from sponge acidic polysaccharide

1970 ◽  
Vol 119 (1) ◽  
pp. 17-19 ◽  
Author(s):  
Richard L. Katzman ◽  
Elwira Lisowska ◽  
Roger W. Jeanloz
Keyword(s):  
Melting Point ◽  
Connective Tissue ◽  
Mixed Melting Point ◽  
Optical Rotation ◽  
Biological Role ◽  
Animal Kingdom ◽  
Rare Sugar ◽  
Acidic Polysaccharide

1. d-Arabinose, an extremely rare sugar in the animal kingdom, was isolated from a complex acidic polysaccharide obtained from the connective tissue of the sponge Hippospongia gossypina. A crystalline derivative of arabinose, the 1-benzyl-1-phenylhydrazone, was prepared and characterized by its melting point, optical rotation, and mixed melting point with authentic d- and l-enantiomers. 2. The distribution of arabinose within the animal kingdom is discussed, and a biological role is proposed for the spongonucleotides.

THE YELLOW COLORING MATTER OF KHAPLI WHEAT, TRITICUM DICOCCUM: II. THE CONSTITUTION OF TRICETIN

1932 ◽  
Vol 7 (3) ◽  
pp. 285-292 ◽  
Author(s):  
J. Ansel Anderson
Keyword(s):  
Melting Point ◽  
Related Compound ◽  
Mixed Melting Point ◽  
Water Soluble ◽  
Wheat Varieties ◽  
Wheat Leaves ◽  
Coloring Matter ◽  
Color Reactions ◽  
Hydrolysis Of ◽  
Derivatives Of

Khapli wheat leaves contain a very small quantity of a trihydroxydimethoxyflavone, tricin. Marquis yields a trace of the same compound. These two varieties also contain water-soluble coloring matters which are apparently glucosides of tricin or of a closely related compound. Dyeing tests carried out with six other wheat varieties indicate that all contain essentially the same coloring matters.Methylation of tricin and hydrolysis of the resulting O-trimethyltricin yielded 3,4,5-trimethoxybenzoic acid and 2-hydroxy-4,6-dimethoxyacetophenone. It was therefore assumed that tricetin, the pentahydroxyflavone derived from tricin, was 5,7,3′,4′,5′-pentahydroxyflavone. This was synthesized from 3,4,5-trimethoxybenzoic acid and phloracetophenone by the Allan-Robinson method. Mixed melting-point determinations showed that its O-pentamethyl and O-penta-acetyl derivatives are identical with the corresponding derivatives of tricetin. The dyeing properties and color reactions of synthetic and natural tricetin are identical and are in fair agreement with those described by Badhwar, Kang and Ventkataraman (3, p. 1111) who recently reported the synthesis of the same compound.

THE NORMAL C17 FATTY ACIDS OF MUSK-OX FAT

1957 ◽  
Vol 35 (12) ◽  
pp. 1434-1437 ◽  
Author(s):  
Mary J. Chisholm ◽  
C. Y. Hopkins
Keyword(s):  
Fatty Acids ◽  
Melting Point ◽  
Mixed Melting Point ◽  
Methyl Esters ◽  
Heptadecanoic Acid ◽  
X Ray Diffraction ◽  
Straight Chain ◽  
Trans Form ◽  
Total Fatty Acids ◽  
Musk Ox

A sample of body fat of the Canadian musk ox (Ovibosmoschatus subsp.) was converted to methyl esters and distilled. The fraction containing esters of C17 acids was crystallized fractionally at low temperature and two straight-chain C17 acids were isolated. n-Heptadecanoic acid was identified by analysis, by mixed melting point of the acid and two derivatives with authentic samples, and by its X-ray diffraction pattern. 9-Heptadecenoic acid was identified by analysis, by mixed melting point of two derivatives with authentic samples, and by oxidative cleavage. It is estimated that the fat contained 1.7% of n-heptadecanoic acid and 0.9% of cis-9-heptadecenoic acid, based on the total fatty acids. Although there was an appreciable content of trans acids in the fat, the heptadecenoic acid was found to contain little or none of the trans form.

Zymogen Secreting Cells in the Hepatopancreatic Duct of Pagurus Bernhardus (Linnaeus, 1758) and Clibanarius Longitarsus (De Haan, 1849) (Anomura)

Crustaceana ◽  
1995 ◽  
Vol 68 (5) ◽  
pp. 616-628
Author(s):  
C. Manjulatha ◽  
D. Erri Babu
Keyword(s):  
Connective Tissue ◽  
Secretory Activity ◽  
Secretory Cells ◽  
Main Duct ◽  
Zymogen Granules ◽  
Electron Microscopic ◽  
Food Material ◽  
Acidic Polysaccharide ◽  
Microscopic Studies ◽  
Pagurus Bernhardus

AbstractHistological and histochemical observations reveal that there are two clusters of acidic polysaccharide secreting glands at the opening of the mouth into the oesophagus in Pagurus bernhardus and Clibanarius longitarsus. Below these there are structurally similar glands in the connective tissue of the oesophagus. The epithelial cells lining the hepatopancreatic main duct show secretory activity and they secrete acidophilic granular secretions, which are chemically similar to vertebrate pancreatic zymogen granules. Electron microscopic studies reveal the presence of secretory cells in the hepatopancreatic duct and the synthesis of zymogens within these cells. The time of release of these granular secretions synchronizes with the release of the food material into the midgut.

scholarly journals Development of chromatographic methods for thebaine detection and quantification along with some of related alkaloid derivatives

2015 ◽  
Vol 34 (2) ◽  
pp. 231
Author(s):  
Marija Spasevska ◽  
Jane B Bogdanov ◽  
Hristina Babunovska
Keyword(s):  
Melting Point ◽  
Optical Rotation ◽  
Monolithic Column ◽  
Gradient Elution ◽  
Spectroscopic Properties ◽  
Hplc Method ◽  
Specific Optical Rotation ◽  
Chromatographic Methods ◽  
Vis Spectroscopy ◽  
Pharmacopoeial Monograph

<p class="Body">Thebaine is naturally occurring alkaloid  which is of great importance in the pharmaceutical industry and  up to now there is not a Pharmacopoeial monograph for this compound.  There is a definite need for development of  simple procedures for routine quality assessment  based on physical and spectroscopic properties (melting point, UV-Vis, IR, specific optical rotation) and even more importantly a need for development of chromatographic methods for determination of  purity.The main objective of this work was to develop RP-HPLC-UVD method for quantification of thebaine and also to develop complementary GC-MS method for qualitative analysis. The developed HPLC method utilized RP-C18 monolithic column, gradient elution with trifluoroacetic acid and formic acid in water and in acetonitrile and detection at 285nm. The effects of the relevant chromatographic conditions was investigated and the optimized and validated method was usеd for analysis of  three batches of thebaine.  </p><p class="Body">A procedure that contained different methods for determination and identification of thebaine, was performed with main focus on the HPLC method suitable for quantitative and qualitative determination.  In parallel, to complete the purity profile,  the three batches of thebaine were analyzed by IR, UV-Vis spectroscopy and specific optical rotation and melting point determination.  The analysis of thebaine can be performed in few steps by simple and reproducible methods like IR spectroscopy, specific optical rotation, melting point and the developed HPLC method, later proved by its validation. The developed HPLC-UV method is suitable for transfer to HPLC-MS method. The GC-MS method is quite suited for identification of thebaine  and of the impurities, but is limited to free bases. Additionally, pseduomorphine  and <em>N</em>-oxide derivatives of the related alkaloids are not suitable for GC-MS analysis.</p>

THE IDENTIFICATION OF A NINHYDRIN-POSITIVE URINARY COMPONENT RECENTLY REPORTED IN HYPOPHOSPHATASIA

1957 ◽  
Vol 35 (12) ◽  
pp. 1123-1133 ◽  
Author(s):  
Donald Fraser ◽  
C. C. Lucas
Keyword(s):  
Absorption Spectrum ◽  
Melting Point ◽  
Diffraction Pattern ◽  
Infrared Absorption ◽  
Mixed Melting Point ◽  
Infrared Absorption Spectrum ◽  
X Ray Diffraction ◽  
X Ray ◽  
Unequivocal Identification ◽  
Affected Infant

In 1955, the occurrence of a hitherto unreported ninhydrin-positive substance in the urine and plasma of infants suffering from hypophosphatasia was described. Evidence published at that time and subsequently suggested that the material might be phosphorylethanolamine, but the isolation and unequivocal identification of the substance has not been reported.The present paper describes the isolation of a small amount of the previously unidentified compound from the urine of the heterozygous father of a seriously affected infant. The melting point, mixed melting point, X-ray diffraction pattern, infrared absorption spectrum, and N: P ratio establish the identity of the substance to be phosphorylethanolamine. Proof that this compound occurs in hypophosphatasia provides a logical basis for further study of the metabolic importance of this substance.

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