scholarly journals The metabolism of [14C]nicotine in the cat

1969 ◽  
Vol 115 (5) ◽  
pp. 889-896 ◽  
Author(s):  
D. M. Turner
Keyword(s):  
Single Dose ◽  
Quantitative Determination ◽  
Intravenous Injection ◽  
Body Fluids ◽  
Small Doses ◽  
The Brain

The metabolism of [2′−14C]nicotine given as an intravenous injection in small doses to anaesthetized and unanaesthetized cats has been studied. A method is described for the quantitative determination of [14C]nicotine and [14C]cotinine in tissues and body fluids. Nanogram amounts of these compounds have been detected. After a single dose of 40μg. of [14C]nicotine/kg., 55% of the injected radioactivity was excreted in the urine within 24hr., but only 1% of this radioactivity was unchanged nicotine. [14C]Nicotine is metabolized extremely rapidly, [14C]cotinine appearing in the blood within 2·5min. of intravenous injection. [14C]Nicotine accumulates rapidly in the brain and 15min. after injection 90% of the radioactivity still represents [14C]nicotine. Metabolites of [14C]nicotine have been identified in liver and urine extracts. [14C]Nicotine-1′-oxide has been detected in both liver and urine.

Application of selected ion monitoring technique for quantitative determination of picomole levels of pyrrolidine in the brain

1981 ◽  
Vol 115 (2) ◽  
pp. 254-259 ◽  
Author(s):  
Yoshiro Okano ◽  
Takeshi Miyata ◽  
Katsunori Iwasaki ◽  
Kazuo Takahama ◽  
Taizo Hitoshi ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Selected Ion Monitoring ◽  
Monitoring Technique ◽  
The Brain

Quantitative determination of T-2 toxin, HT-2 toxin, deoxynivalenol and deepoxy-deoxynivalenol in animal body fluids using LC–MS/MS detection

2011 ◽  
Vol 879 (24) ◽  
pp. 2403-2415 ◽  
Author(s):  
S. De Baere ◽  
J. Goossens ◽  
A. Osselaere ◽  
M. Devreese ◽  
V. Vandenbroucke ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Body Fluids ◽  
Animal Body ◽  
Ms Detection

The New Wheat MDH-GOAT Enzyme Complex and its Application for Quantitative Determination of Glutamate Concentration

2013 ◽  
Vol 781-784 ◽  
pp. 957-960
Author(s):  
Ibragimova Saniyam ◽  
Elena Gukkengeimer ◽  
Nataliya Riger ◽  
Gulzat Kulbaeva ◽  
Murat Gilmanov
Keyword(s):  
Quantitative Determination ◽  
Enzyme Preparation ◽  
Bovine Liver ◽  
Enzyme Complex ◽  
Reliable Determination ◽  
Glutamate Concentration ◽  
High Lability ◽  
The Republic ◽  
The Brain

The quantitative determination of glutamate is very important for diagnostic of many diseases of the nervous system, severity of stroke of the brain and also for determination glutamate in the food stuffs [1,2]. To the present days for quantitative determination of glutamate is wide used to the enzyme preparation of glutamate dehydrogenase (GDh) from bovine liver. However the application of GDh has the next serious disadvantages: high lability of this preparation and many substances strong change the activity of GDh. For example there are nucleotides, amino acid, steroid and metal ions. As the results the application of GDh preparation dont give reliable determination of quantity of glutamate. Thus there is the high necessity to propose another enzyme preparation for quantitative determination of glutamate without above mentioned disadvantages. In this reason we propose absolutely new enzyme preparation for this aim. In the laboratory of the enzyme structure and regulation of the Institute of the molecular biology and biochemistry of the Ministry of the education and science of the Republic of Kazakhstan was discovered the new enzyme complex (EC), which consists of malate dehydrogenase (MDh) and glutamate-oxaloacetate aminotransferase (GOAT) and used it for quantitative determination of glutamate concentration [3].

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