A general method for the quantitative determination of catecholamines in body fluids using off-line sample pretreatment and HPLC-ED analysis

1989 ◽  
Vol 28 (1-2) ◽  
pp. 78-84 ◽  
Author(s):  
H. -J. Bauch ◽  
E. Strüwer ◽  
U. Kelsch
Keyword(s):  
Quantitative Determination ◽  
Sample Pretreatment ◽  
Body Fluids ◽  
Line Sample ◽  
General Method

Quantitative determination of T-2 toxin, HT-2 toxin, deoxynivalenol and deepoxy-deoxynivalenol in animal body fluids using LC–MS/MS detection

2011 ◽  
Vol 879 (24) ◽  
pp. 2403-2415 ◽  
Author(s):  
S. De Baere ◽  
J. Goossens ◽  
A. Osselaere ◽  
M. Devreese ◽  
V. Vandenbroucke ◽  
...  
Keyword(s):  
Quantitative Determination ◽  
Body Fluids ◽  
Animal Body ◽  
Ms Detection

Application of 31P NMR for added polyphosphate determination in pork meat

2009 ◽  
Vol 63 (4) ◽  
Author(s):  
Aneta Jastrzębska ◽  
Edward Szłyk
Keyword(s):  
Quantitative Determination ◽  
31P Nmr ◽  
Sample Pretreatment ◽  
Phosphorus Compounds ◽  
Pork Meat ◽  
Qualitative And Quantitative ◽  
Minimal Sample ◽  
Meat Samples ◽  
Phosphorus Determination

AbstractApplication of 31P NMR for qualitative and quantitative determination of added phosphorus compounds in meat samples is described. Furthermore, usefulness of the proposed method for monitoring of poly- and pyrophosphates hydrolysis in meat is discussed. Calibration curves based on the 31P resonance line areas were elaborated for Na3P3O9, Na5P3O10, Na2H2P2O7, and K4P2O7 resulting in linearity (R 2 = 0.9976, 0.9953, 0.9974, and 0.9524, respectively), detection limits (DL from 0.0018 mol L−1 for Na3P3O9 to 0.0070 mol L−1 for K4P2O7), and quantification limits (QL from 0.0060 mol L−1 for Na3P3O9 to 0.0234 mol L−1 for K4P2O7). The developed procedure was applied for laboratory prepared meat samples and compared with the standard UV-VIS method. The minimal sample pretreatment, obtained within-day precision (CV ≤ 2.0 %) and accuracy (as recovery ≥ 95 %) suggest 31P NMR as an alternative method of phosphorus determination in food analysis.

New "on-line" sample-pretreatment procedure for routine liquid-chromatographic assay of low-concentration compounds in body fluids, illustrated by triamcinolone assay.

1983 ◽  
Vol 29 (7) ◽  
pp. 1367-1371 ◽  
Author(s):  
M Schöneshöfer ◽  
A Kage ◽  
B Weber
Keyword(s):  
Low Cost ◽  
Sample Pretreatment ◽  
Body Fluids ◽  
Analytical Recovery ◽  
On Line ◽  
Internal Standardization ◽  
Automated Technique ◽  
Universal Applicability ◽  
Liquid Chromatographic ◽  
Line Sample

Abstract In this fully automated technique for sample cleanup before chromatographic or other quantitation steps, analytes in body fluids are enriched and semi-purified on a first column. After their selective elution, analytes are "transformed" by admixing appropriate solvents in such a way that they are focused on the top of a second column. By backflush, they then are transferred to an analytical liquid-chromatographic column (or simply eluted for quantification by other techniques). This technique is illustrated by the liquid-chromatographic assay of triamcinolone from a 1-mL urine sample, with ultraviolet detection. Because analytical recovery is almost complete and precision high, no internal standardization is necessary. Interference is eliminated as well as or better than with manual techniques. Chief advantages of this technique are online operation, processing of samples of larger volume, low cost with respect to extraction devices, and nearly universal applicability for exogenous or endogenous compounds of clinical relevance. It potentially may be widely applied.

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