Effect of light intensity and wavelengths on ascorbic acid content and the antioxidant system in tomato fruit grown in vitro

2020 ◽  
Vol 274 ◽  
pp. 109673
Author(s):  
Kazufumi Zushi ◽  
Chisato Suehara ◽  
Minori Shirai
Keyword(s):  
Ascorbic Acid ◽  
Light Intensity ◽  
Antioxidant System ◽  
Acid Content ◽  
Tomato Fruit ◽  
Ascorbic Acid Content ◽  
Effect Of Light

The role of light intensity in mediating ascorbic acid content during postharvest tomato ripening: A transcriptomic analysis

2021 ◽  
Vol 180 ◽  
pp. 111622
Author(s):  
Yating Zhang ◽  
Nikolaos Ntagkas ◽  
Dimitrios Fanourakis ◽  
Georgios Tsaniklidis ◽  
Jiantao Zhao ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Light Intensity ◽  
Acid Content ◽  
Ascorbic Acid Content ◽  
Transcriptomic Analysis ◽  
Role Of Light

scholarly journals Antioxidant, Anti-Inflammatory and Anti-Angiogenic Properties of Citrus lumia Juice

2020 ◽  
Vol 11 ◽  
Author(s):  
Antonella Smeriglio ◽  
Marcella Denaro ◽  
Valeria D’Angelo ◽  
Maria Paola Germanò ◽  
Domenico Trombetta
Keyword(s):  
Ascorbic Acid ◽  
Acid Content ◽  
Molecular Mechanisms ◽  
Biological Effects ◽  
Ascorbic Acid Content ◽  
Zebrafish Embryos ◽  
Anti Inflammatory ◽  
Dose Dependent

Citrus juices are a rich source of bioactive compounds with various and well-known health benefits. The aim of this study was to investigate the polyphenols and ascorbic acid content as well as to investigate the antioxidant, anti-inflammatory and anti-angiogenic properties of the juice of an ancient Mediterranean species, Citrus lumia Risso (CLJ). The antioxidant and anti-inflammatory activities were evaluated by several in vitro cell-free and cell-based assays, whereas two different in vivo models, the chick chorioallantoic membrane (CAM) and the zebrafish embryos, were used to characterize the anti-angiogenic properties. Twenty-eight polyphenols were identified by RP-LC-DAD-ESI-MS analysis (flavonoids 68.82% and phenolic acids 31.18%) with 1-caffeoyl-5-feruloylquinic acid and kaempferol 3′-rhamnoside, which represent the most abundant compounds (25.70 and 23.12%, respectively). HPLC-DAD analysis showed a high ascorbic acid content (352 mg/kg of CLJ), which contributes with polyphenols to the marked and dose-dependent antioxidant and anti-inflammatory properties observed. CLJ showed strong and dose-dependent anti-angiogenic activity as highlighted by the inhibition of blood vessel formation on CAMs and the decrease of endogenous alkaline phosphatase on zebrafish embryos. Moreover, within the concentration range tested, no dead or malformed embryos were recorded. Certainly, further studies are needed to investigate the molecular mechanisms underlying these promising biological effects, but considering the evidence of the present study, the use of CLJ as a ready-to drink safe prevention strategy for inflammatory-based diseases correlated to angiogenesis could be justified.

scholarly journals Obtaining Salt Stress-Tolerant Eggplant Somaclonal Variants from In Vitro Selection

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2539
Author(s):  
Sami Hannachi ◽  
Stefaan Werbrouck ◽  
Insaf Bahrini ◽  
Abdelmuhsin Abdelgadir ◽  
Hira Affan Siddiqui ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Salt Stress ◽  
Water Content ◽  
Acid Content ◽  
In Vitro Selection ◽  
Callus Tissue ◽  
Salt Content ◽  
Ascorbic Acid Content ◽  
Control Line

An efficient regeneration protocol was applied to regenerate shoots on salt stress-tolerant calli lines of aubergine (Solanum melongena). These NaCl-tolerant cell lines were obtained by two different methods. On the one hand, the developed callus tissue was transferred to a medium with a continuous salt content of 40, 80, 120, or 160 mM NaCl. On the other hand, the callus tissue was subjected to a stepwise increasing salinity to 160 mM NaCl every 30 days. With the second method, calli which could be selected were characterized by compact growth, a greenish color, and absence of necrotic zones. When grown on salt-free medium again, NaCl-tolerant calli showed a decline in relative growth rate and water content in comparison to the control line. This was more obvious in the 120 mM NaCl-tolerant callus. Lipid peroxidase activity increased in 40 and 80 mM NaCl-tolerant calli; yet did not increase further in 120 mM-tolerant callus. An increase in ascorbic acid content was observed in 80 and 120 mM NaCl-tolerant calli compared to the 40 mM NaCl-tolerant lines, in which ascorbic acid content was twice that of the control. All NaCl-tolerant lines showed significantly higher superoxide dismutase (SOD) (208–305–370 µmol min−1 mg−1 FW) and catalase (CAT) (136–211–238 µmol min−1 mg−1 FW) activities compared to control plants (231 and 126 µmol min−1 mg−1 FW). Plants were regenerated on the calli lines that could tolerate up to 120 mM NaCl. From the 32 plants tested in vitro, ten plants with a higher number of leaves and root length could be selected for further evaluation in the field. Their high salt tolerance was evident by their more elevated fresh and dry weight, their more increased relative water content, and a higher number and weight of fruits compared to the wild-type parental control. The presented work shows that somaclonal variation can be efficiently used to develop salt-tolerant mutants.

scholarly journals Grafting and Shading—The Influence on Postharvest Tomato Quality

Agriculture ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 181
Author(s):  
Zoran S. Ilić ◽  
Athanasios Koukounaras ◽  
Lidija Milenković ◽  
Žarko Kevrešan ◽  
Aleksandra Bajić ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Succinic Acid ◽  
Acid Content ◽  
Tomato Fruit ◽  
Storage Period ◽  
Principal Component ◽  
Ascorbic Acid Content ◽  
Fruit Composition ◽  
Fruit Skin ◽  
Tomato Cultivars

Interaction of grafting and shading on tomato physical properties and chemical composition after 15 days of storage at 10 °C and 90% relative humidity was investigated in ungrafted and grafted tomato cultivars ‘Optima F1’ and ‘Big beef F1’ grown under shading nets (red and pearl net) and nonshaded conditions. For grafted plants ‘Maxifort’ rootstock was used. The effects of two weeks of storage was statistically significant when taking into account the effects of grafting, shading and variety for all tomato fruit composition parameters, except total phenols. A principal component analysis demonstrated that the changes in tomato fruit traits during the studied storage period were the main source of differentiation in tomato fruit quality. Beside a slight loss of firmness, tomato fruits were generally expected to have lower lycopene, sugar, malic and citric acid contents, higher succinic acid content, more elastic fruit skin and higher ascorbic acid content. Additionally, after storage, fruits from grafted plants had lower total phenol, higher ascorbic acid and higher succinic acid contents compared to fruits from ungrafted plants. Storage diminishes the differences in quality achieved through convenient grafting and shading combinations.

Anoxia-resistant turtle brain maintains ascorbic acid content in vitro

1991 ◽  
Vol 132 (2) ◽  
pp. 141-145 ◽  
Author(s):  
Margaret E. Rice ◽  
Jon Cammack
Keyword(s):  
Ascorbic Acid ◽  
Acid Content ◽  
Ascorbic Acid Content
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