scholarly journals Changes in dry weight, protein, deoxyribonucleic acid, ribonucleic acid and reserve and structural carbohydrate during the aerobic growth cycle of yeast

1966 ◽  
Vol 98 (3) ◽  
pp. 883-887 ◽  
Author(s):  
ES Polakis ◽  
W Bartley
Keyword(s):  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Dry Weight ◽  
Growth Cycle ◽  
Aerobic Growth ◽  
Structural Carbohydrate ◽  
Weight Protein

CHANGES IN THE DRY WEIGHT, PROTEIN, NUCLEIC ACID, AND CHLOROPHYLL CONTENTS OF GROWING PEA LEAVES

1961 ◽  
Vol 39 (4) ◽  
pp. 891-900 ◽  
Author(s):  
Robert M. Smillie ◽  
G. Krotkov
Keyword(s):  
Ribonucleic Acid ◽  
Soluble Protein ◽  
Deoxyribonucleic Acid ◽  
Higher Plants ◽  
Growth Period ◽  
Dry Weight ◽  
Cell Number ◽  
Chlorophyll Contents ◽  
Weight Protein ◽  
Protein Nucleic Acid

Pea leaves of different ages were harvested over a 10-day growth period and were analyzed for the following: fresh weight, dry weight, cell number, chlorophyll, soluble protein, ribonucleic acid, and deoxyribonucleic acid. The chlorophyll content increased rapidly during early leaf expansion, then remained fairly constant. Only small differences were found in the percentage of soluble protein in the leaves during their development. In contrast, the ribonucleic acid and deoxyribonucleic acid levels in the leaves decreased continuously as the leaves aged. These results are discussed in relation to similar analyses performed on leaves from other higher plants.

The physiology of growth in Subterranean clover. II. The dynamics of leaf growth

1970 ◽  
Vol 18 (2) ◽  
pp. 149 ◽  
Author(s):  
RF Williams ◽  
. Rijven.A.H.G.C
Keyword(s):  
Cell Wall ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Vascular System ◽  
Leaf Growth ◽  
Subterranean Clover ◽  
Dry Weight ◽  
Protein Nitrogen ◽  
Wall Materials ◽  
Cell Wall Materials

This study extends the account of the development of the fourth leaf of subterranean clover. In particular, the four leaf constituents deoxyribonucleic acid, ribonucleic acid, proteins, and cell wall materials are expressed on whole leaf and cell bases. Form change in the leaf is illustrated with perspective elevations for some early stages. Cell number increases exponentially until day 13, with a mean generation time of 18 hr. The final number per leaf is c. 6.5 million, and deoxyribonucleic acid phosphorus is estimated as 0.197 pg per cell. The leaf is highly succulent, with a maximum water content of 478 % dry weight. Soluble constituents are also high, with a maximum of 39 % of the dry matter. The absolute amounts of ribonucleic acid phosphorus and protein nitrogen are maximal on days 19 and 25 respectively, with losses of about 60% by day 36. Cell wall materials increase from 8 to 57 % of the residual dry weight during a period of 4 days prior to emergence. This is shown to be due to the concurrent differentiation of the vascular system and the growth of 3000 leaf hairs. Changes on a per cell basis are recorded, and the concept of relative growth rate is used to extend the analysis of the data. Rates of production of one constituent per unit of another are also presented and discussed.

Composition of Methanospirillum hungatii GP1 during growth on different media

1980 ◽  
Vol 26 (5) ◽  
pp. 577-582 ◽  
Author(s):  
C. Breuil ◽  
G. B. Patel
Keyword(s):  
Protein Content ◽  
Ribonucleic Acid ◽  
Acid Content ◽  
Deoxyribonucleic Acid ◽  
Synthetic Medium ◽  
Density Measurement ◽  
Dry Weight ◽  
Weight Basis ◽  
Total Carbohydrate ◽  
Mineral Salts

Growth of Methanospirillum hungatii GP1 as determined by optical density measurement was comparable to growth assessed by cell dry weight, ribonucleic acid content, and deoxyribonucleic acid content. Cultivation of M. hungatii on synthetic medium containing mineral salts, vitamins, and acetic acid indicated that, on a dry weight basis, cell constituents such as protein (71%), ribonucleic acid (15.8%), deoxyribonucleic acid (1.6%), and total carbohydrate (3.2%) did not vary significantly with the growth phase. Cells grown in the synthetic medium supplemented with yeast extract and tryptone had slightly higher protein content (76%), but the concentrations of the other cell constituents were similar and did not fluctuate much during growth. Nitrogen limiting growth resulted in somewhat lower ribonucleic acid content as well as slightly higher protein content than that in cells grown in nonlimiting medium. Methanospirillum hungatii did not accumulate any of the commonly known reserve materials under nitrogen or carbon and hydrogen limiting growth.

Aspects of metabolic development in an illuminated synchronous culture of Scenedesmus obtusiusculus

1973 ◽  
Vol 51 (1) ◽  
pp. 113-120 ◽  
Author(s):  
Gaurangakumar Das
Keyword(s):  
Growth Phase ◽  
Ribonucleic Acid ◽  
Culture Medium ◽  
Deoxyribonucleic Acid ◽  
Dry Weight ◽  
Light Period ◽  
Weight Basis ◽  
Lipid Phosphorus ◽  
Acid Phosphate ◽  
Cellular Development

Developing synchronous cells of Scenedesmus obtusiusculus Chod. were investigated during the 15-h light period of the 24-h life cycle. Measurements were made of photosynthesis and of the chlorophyll, carotene, nucleic acid, and protein content, including intracellular phosphorus.Photosynthetic oxygen evolution (μmol/mg chlorophyll) increased during the early growing stage and declined gradually in the older cells. Chlorophyll, carotene, protein, ribonucleic acid, and ribonucleic acid phosphate of these cells increased during the growth phase (dry weight basis) and declined in the sporulating phase, whereas the deoxyribonucleic acid and deoxyribonucleic phosphate decreased during the growth phase. Deoxyribonucleic acid increased slightly in the sporulating phase, but deoxyribonucleic acid phosphate remained relatively constant during this period. When calculated on a per cell basis, ribonucleic acid and ribonucleic acid phosphate increased steadily throughout the period of observation, whereas deoxyribonucleic acid and deoxyribonucleic acid phosphate remained constant during the first 5 h and then increased gradually. Analysis of the intracellular distribution of phosphorus(dry weight basis) revealed that methanol-soluble (lipid) phosphorus decreased steadily with cell growth, whereas the content of acid-soluble phosphorus and ethanol–ether-soluble (lipid) phosphorus fluctuated markedly. The pH of the culture medium increased concomitantly with cellular development during the light period.

scholarly journals The Enzymatic Phosphorylation of Ribonucleic Acid and Deoxyribonucleic Acid

1966 ◽  
Vol 241 (12) ◽  
pp. 2933-2943 ◽  
Author(s):  
Abraham Novogrodsky ◽  
Moshe Tal ◽  
Abraham Traub ◽  
Jerard Hurwitz
Keyword(s):  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Enzymatic Phosphorylation
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