scholarly journals Gas–liquid partition chromatography: the separation and micro-estimation of volatile fatty acids from formic acid to dodecanoic acid

1955 ◽  
Vol 61 (1) ◽  
pp. 174-176 ◽  
Author(s):  
J. H. Van De Kamer ◽  
K. W. Gerritsma ◽  
E. J. Wansink
1953 ◽  
Vol 43 (3) ◽  
pp. 260-264 ◽  
Author(s):  
A. J. G. Barnett ◽  
R. E. B. Duncan

1. The use of the technique of gas-partition chromatography in the investigation of the formation of volatile acids in minced grass/water slurries is discussed.2. Slurries kept anaerobically at 17, 37 and 62° for 13 weeks have been examined, and the amounts of the different volatile acids formed have been estimated. It has been noted that butyric acid forms most readily in the slurry at 37°, while in aerated slurries at 17°, the formation of volatile fatty acids is more speedy than under anaerobic conditions.3. Only acetic, propionic and butyric acids have been noted so far, and the highest concentrations of the acids observed in any slurry were 5, 1·25 and 4% respectively.


Nature ◽  
1950 ◽  
Vol 166 (4221) ◽  
pp. 522-522 ◽  
Author(s):  
E. R. HISCOX ◽  
N. J. BERRIDGE

1921 ◽  
Vol 3 (3) ◽  
pp. 325-330 ◽  
Author(s):  
Harper F. Zoller ◽  
W. Mansfield Clark

These studies show: 1. A close agreement exists among all the organisms studied in the total quantity of volatile fatty acids produced and in the ratio of formic to acetic, under aerobic conditions, and in the presence of 1 per cent of glucose. 2. When grown upon peptone alone, with free access of air to the cultures, volatile fatty acids are produced in appreciable quantities, although the reaction of the solution has gone more alkaline as shown by colorimetric pH tests. Formic acid is not found, but in its place we obtain propionic acid. 3. Upon exhaustion of air from the non-sugar medium the bacteria again produce formic acid, and in addition some butyric. This is true for both Shiga and non-Shiga cultures. The reaction is distinctly more acid. 4. The presence of glucose in the medium from which the air has been pumped furnishes a condition which provokes about the same type and degree of fermentation that operates in the glucose medium bathed in air at atmospheric pressure. 5. The enormous quantity of formic acid produced by these bacteria may play a significant part in the digestive disturbances and toxic symptoms accompanying their infection of the human intestinal tract.


1977 ◽  
Vol 25 (1) ◽  
pp. 71-81 ◽  
Author(s):  
Elizabeth Donaldson ◽  
R. A. Edwards

SUMMARYThe nutritive value of four silages was compared using eight rumenfistulated wethers. Wilted ryegrass was ensiled without additive (A); with formic acid (13·9 kg/t dry matter) (B); with formic acid (10·3 kg/t dry matter) plus formalin (formaldehyde at 4·8 kg/t dry matter) (C); with formic acid (4·9 kg/t dry matter) plus formalin (formaldehyde at 2·3 kg/t dry matter) plus propionic acid (5·6 kg/t dry matter) (D).Dry-matter intakes were 13·8, 15·1, 14·3 and 14·3 g/kg live weight for A, B, C and D respectively. Digestibilities of organic matter and dry matter were not affected by the additives. Digestibility of nitrogen and peak ruminal ammonia concentration were significantly (P<0·05) reduced by the addition of formaldehyde.The effect on rumen fermentation characteristics, of chemically restricting in-silo fermentation, is illustrated. Differences in the rumen volatile fatty acids and in the efficiency of utilization of the energy for growth, fattening and lactation are discussed.


1992 ◽  
Vol 72 (2) ◽  
pp. 431-434 ◽  
Author(s):  
P. Narasimhalu ◽  
L. J. Halliday ◽  
J. B. Sanderson ◽  
H. T. Kunelius ◽  
K. A. Winter

Timothy was field-wilted and ensiled in plastic-tube silos either untreated or treated with formic acid or a cellulase-hemicellulase preparation (enzyme). This study showed that first-cut timothy, field wilted to 350 g kg−1 DM, can be stored as a medium- to good-quality silage without using formic acid or enzyme as an additive. Key words: Silage composition, intake, digestibility, volatile fatty acids


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