scholarly journals The distribution and interrelationships of the alkaloids in the bark of Cinchona Ledgeriana

1948 ◽  
Vol 43 (1) ◽  
pp. 38-44 ◽  
Author(s):  
H. F. Birch ◽  
L. R. Doughty
Keyword(s):  
Cinchona Ledgeriana

scholarly journals Screening of secondary metabolites quinine alkaloid by endophytic bacteria from cinchona plants (Cinchona ledgeriana moens.) root

2021 ◽  
Author(s):  
Fauzi Akhbar Anugrah ◽  
Satrio Anggoro Putra ◽  
Sulisetijono Sulisetijono ◽  
Sitoresmi Prabaningtyas ◽  
Hanumi Oktyani Rusdi
Keyword(s):  
Secondary Metabolites ◽  
Endophytic Bacteria ◽  
Cinchona Ledgeriana

scholarly journals Alkaloids Production and Cell Growth of Cinchona ledgeriana Moens: Effects of Fungal Filtrate and Methyl Jasmonate Elicitors

2021 ◽  
Vol 6 (1) ◽  
pp. 31-40
Author(s):  
Yustiny Andaliza Hasibuan ◽  
Diah Ratnadewi ◽  
Zainal Alim Mas’ud
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Secondary Metabolites ◽  
Methyl Jasmonate ◽  
Woody Plant ◽  
Cultured Cells ◽  
Cinchona Alkaloids ◽  
Cell Biomass ◽  
Cinchona Ledgeriana ◽  
Culture Technology

Cinchona alkaloids are known as antimalaria and anti-arrhythmic. Due to the long waiting time to harvest, cell culture technology is a challenge. This study aimed to determine the effects of elicitors, filtrate of two strains of endophytic fungi and methyl jasmonate (MeJA), in cell suspension culture of Cinchona ledgeriana on quinine and quinidine production. The cells were cultured for seven weeks in woody plant (WP) media treated with either of those elicitors in various concentrations. The cells growth was observed and the alkaloids were analyzed by HPLC. Cells treated with MeJA failed to grow that led to the cell biomass insufficiency for alkaloids determination.  It indicates that the cells are quite sensitive to even low concentration of MeJA that hampered the growth. Cells treated with the filtrate of Diaporthe sp. M13-Millipore filtered (S2M) gave the least cell biomass but presented the highest content of both alkaloids. Diaporthe sp. strain M-13 is stronger as elicitor than M-23 for this plant species. Filtrate of non-virulent fungi can elevate the biosynthesis of alkaloids. This reconfirms that cultured cells are capable to produce secondary metabolites and the productivity can be increased by using an appropriate elicitor.  

scholarly journals Bioproduction of Cinchona Alkaloids by the Endophytic Fungus Diaporthe sp. Associated with Cinchona ledgeriana

2012 ◽  
Vol 60 (10) ◽  
pp. 1301-1304 ◽  
Author(s):  
Shoji Maehara ◽  
Partomuan Simanjuntak ◽  
Chinami Kitamura ◽  
Kazuyoshi Ohashi ◽  
Hirotaka Shibuya
Keyword(s):  
Endophytic Fungus ◽  
Cinchona Alkaloids ◽  
Cinchona Ledgeriana

scholarly journals Teknik sambung mikro in vitro kina Cinchona succirubra dengan C. ledgeriana In vitro micrografting technique of Chincona succirubra and C. ledgeriana

2016 ◽  
Vol 74 (2) ◽  
Author(s):  
Nurita TORUAN-MATHIUS ◽  
. LUKMAN ◽  
. AGUS-PURWITO
Keyword(s):  
Tissue Culture ◽  
Clonal Propagation ◽  
Ms Medium ◽  
Randomized Design ◽  
Top Soil ◽  
Completely Randomized Design ◽  
Reduced Light ◽  
In Vitro Micrografting ◽  
Cinchona Ledgeriana

Summary In vitro micrografting is a technique for grafting scions to rootstocks of plantlets from tissue culture. In vitro micrografting of Cinchona plant has never been carried out. The objective of this research was to obtain the best method of in vitro micrografting, medium for micrografted plantlets, and acclimatization  for Cinchona plantlets from  micrografting. The research consisted of (i) optimization of micrografting method, (ii) optimization of medium for growing plantlets, and (iii) acclimatization of micrografted plantlet. Plantlets of four-month-old of  C. ledgeriana  QRC clone were used as  scions, while of C. succirubra as  rootstocks. Each of experiments was arranged according to Completely Randomized Design, consisted of  combination of scion and rootstock and type of micro-grafting with 10 replicates. Parameters measured were  the percentage of survived plantlet, leaf number, and callus productions on union area, and percentage of survived  plantlet. The results show that V type of micrografting was the best for Cinchona micrografting. MS medium with the addition of 3 mg/L IBA was the best medium for growing of micrografted plantlet. Husk charcoal mixed with top soil (1 : 1) was the best medium for acclimatization.  Acclimatization  consisted  of two steps: preaclimatization in a culture room with 12- hour photoperiod at temperature 25 – 27oC  for two weeks,  followed by aclimatization in a plastic house with  70% reduced light intensity for one month. Using this method, 90% of the seedlings were survived. It is concluded that in vitro micrografting can be used as a technique for clonal propagation of Cinchona sp.Ringkasan  Teknik sambung mikro (mikrografting) in vitro adalah teknik penyambungan potongan batang atas pada batang bawah dalam kultur jaringan.  Pada tanaman kina teknik sambung mikro  in vitro belum pernah dilakukan. Tujuan penelitian ini adalah  menetapkan tipe sambung mikro, medium terbaik untuk planlet hasil sambung  mikro, dan perbanyakan tanaman kina dengan sambung mikro. Pelaksanaan percobaan meliputi (i) optimasi tipe sambung, (ii) optimasi  medium, dan (iii) aklimatisasi planlet hasil sambung mikro. Bahan tanaman yang digunakan sebagai batang atas adalah planlet Cinchona ledgeriana klon QRC, sedangkan sebagai batang bawah digunakan planlet  C. succirubra, berumur empat bulan. Masing- masing percobaan disusun dengan Rancangan Acak Lengkap terdiri dari dua taraf yaitu  kombinasi batang bawah dengan batang atas bentuk sambung tipe V dan L dilakukan  dengan 10 ulangan. Peubah yang diukur meliputi persentase planlet yang bertahan hidup,  jumlah daun,  berkalus atau tidak berkalus pada daerah pertautan, dan persentase planlet yang bertahan hidup. Hasil yang diperoleh menunjukkan bahwa tipe V merupakan cara sambung  mikro  yang terbaik. Medium MS dengan penambahan 3 mg/L IBA adalah medium terbaik untuk pertumbuhan dan perakaran planlet hasil sambung mikro.  Aklimatisasi planlet dilakukan dengan medium tumbuh arang sekam : top soil (1 : 1) yang disterilkan. Tahapan aklimatisasi adalah pre-aklimatisasi dalam ruang kultur  suhu 25 -     27 oCdengan pencahayaan 12 jam per hari dan diikuti dengan aklimatisasi di rumah plastik bernaungan 70% paranet. Dengan metode aklimatisasi ini  90% dari bibit mampu bertahan hidup. Kesimpulan dari penelitian ini menunjukkan bahwa teknik sambung mikro dapat digunakan untuk perbanyakan klonal   Cinchona sp..

scholarly journals The effect of spacing and thinning on the yield of Cinchona ledgeriana.

1953 ◽  
Vol 1 (1) ◽  
pp. 44-53
Author(s):  
K. Ebes
Keyword(s):  
Quinine Sulfate ◽  
Preliminary Conclusion ◽  
Japanese Occupation ◽  
Slight Tendency ◽  
Close Spacing ◽  
Average Production ◽  
The Difference ◽  
Cinchona Ledgeriana

The layout of an experiment started in Indonesia in 1933 to determine the effect of spacing and thinning on yield of Cinchona ledgeriana is described. The experiment was planned to last 25 years but was interrupted in 1942 by the Japanese occupation. Data recorded during these first 9 years are tabulated and the results summarized as follows. The production of clone Tjib. 5 was about 20% higher than that of Tjin. 1. Planting of 7, 200 and 8, 600 trees per ha. produced less than the widest spaced plantings of 5, 000 per ha. and the closest spaced plantings of 12, 600 per ha. The difference in yield between plots of very different spacings was relatively small, but the plots with the densest stand gave the greatest average production of quinine sulfate. The economics of dense planting are discussed. Height of tree was found to be practically independent of spacing, in contrast to girth which was a function of it. Close spacing produced slender trees with thin bark, but expressed as a percentage of the radius such bark was not relatively thinner than that of widely spaced trees. The ring content of quinine (pure quinine in dry bark from samples cut 1 m. above the ground) showed a slight tendency to decrease with age, after reaching a maximum in the sixth year. The preliminary conclusion is reached that, with regular thinning and fertilization, close spacing of cinchona is technically desirable and gives the highest yield. (Abstract retrieved from CAB Abstracts by CABI’s permission)

Sign in / Sign up

Export Citation Format

Share Document