Use of a surfactant selective electrode in the measurement of the binding of anionic surfactants to bovine serum albumin

1976 ◽  
Vol 72 (0) ◽  
pp. 481 ◽  
Author(s):  
Henry M. Rendall
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Anionic Surfactants ◽  
Selective Electrode

scholarly journals Fluorescence Spectroscopy Study on the Interaction of Acetal Cleavable Anionic Surfactants and Bovine Serum Albumin

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Xin Zhang ◽  
Jianhong Bian ◽  
Wenjie Zhai ◽  
Jing Dong ◽  
Huihui Liang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Fluorescence Spectroscopy ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Protein Separation ◽  
Anionic Surfactants ◽  
Binding Constants ◽  
Number Of Binding Sites ◽  
Hydrophobic Nature ◽  
Thermodynamic Relationship

The interactions between bovine serum albumin (BSA) and two cleavable anionic surfactants, sodium 3-[(2-nonyl-1,3-dioxolan-4-yl)methoxy]propane-1-sulfonate (SNPS) and sodium 3,3′-(2-nonyl-1,3-dioxane-5,5-diyl)bis(methylene)bis(oxy)dipropane-1-sulfonate (SNDPS), have been studied by means of fluorescence spectroscopy and thermodynamic analysis. The fluorescence of BSA is quenched via a static quenching mechanism with the addition of the surfactants. The binding constants of the surfactants and proteins have been measured, with KA(SNPS) = 8.71×104 M−1 and KA(SNDPS) = 7.08 × 104 M−1, respectively. The interaction between surfactants and BSA is mainly of hydrophobic nature, based on the number of binding sites, n[n(SNPS) = 1.57, n(SNDPS) = 1.47], and the thermodynamic relationship. These results suggest that SNPS and SNDPS could be effective protein denaturants for protein separation and analysis.

Interaction of bovine serum albumin with anionic surfactants

2001 ◽  
Vol 3 (20) ◽  
pp. 4583-4591 ◽  
Author(s):  
Shashank Deep ◽  
Jagdish C. Ahluwalia
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Anionic Surfactants

Interactions of anionic surfactants with poly(ethylene oxide) and bovine serum albumin polymers: effect of the counterion hydrophobicity

1999 ◽  
Vol 147 (1-2) ◽  
pp. 89-105 ◽  
Author(s):  
Dino Zanette ◽  
Cláudio F Lima ◽  
Ângelo A Ruzza ◽  
Alexanders T.N Belarmino ◽  
Sônia de F. Santos ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Ethylene Oxide ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Anionic Surfactants ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene
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