Investigation of Cu(II) Binding to Bovine Serum Albumin by Potentiometry with an Ion Selective Electrode: A Biophysical Chemistry Experiment for the Undergraduate Curriculum

2004 ◽  
Vol 81 (3) ◽  
pp. 395 ◽  
Author(s):  
Jie Liu
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Undergraduate Curriculum ◽  
Ion Selective Electrode ◽  
Selective Electrode ◽  
Chemistry Experiment ◽  
Biophysical Chemistry

CaCO3 nanoparticles as the tags for potentiometric detection of a cardiac biomarker using a calcium ion-selective electrode

2017 ◽  
Vol 9 (47) ◽  
pp. 6609-6612 ◽  
Author(s):  
Xiaohong Fu ◽  
Kun Xu ◽  
Xueyu Feng ◽  
Bo Chang ◽  
Fan Yang ◽  
...  
Keyword(s):  
Calcium Carbonate ◽  
Bovine Serum Albumin ◽  
Calcium Ion ◽  
Bovine Serum ◽  
Ion Selective Electrode ◽  
Signal Generation ◽  
Selective Electrode ◽  
Cardiac Biomarker ◽  
Potentiometric Detection ◽  
Caco3 Nanoparticles

A novel potentiometric immunosensing protocol was developed for the detection of myoglobin on a calcium ion-selective electrode by using bovine serum albumin-functionalized calcium carbonate nanoparticles as the signal-generation tags.

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

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