Interaction of bovine serum albumin with anionic surfactants

2001 ◽  
Vol 3 (20) ◽  
pp. 4583-4591 ◽  
Author(s):  
Shashank Deep ◽  
Jagdish C. Ahluwalia
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Anionic Surfactants

scholarly journals Fluorescence Spectroscopy Study on the Interaction of Acetal Cleavable Anionic Surfactants and Bovine Serum Albumin

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Xin Zhang ◽  
Jianhong Bian ◽  
Wenjie Zhai ◽  
Jing Dong ◽  
Huihui Liang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Fluorescence Spectroscopy ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Protein Separation ◽  
Anionic Surfactants ◽  
Binding Constants ◽  
Number Of Binding Sites ◽  
Hydrophobic Nature ◽  
Thermodynamic Relationship

The interactions between bovine serum albumin (BSA) and two cleavable anionic surfactants, sodium 3-[(2-nonyl-1,3-dioxolan-4-yl)methoxy]propane-1-sulfonate (SNPS) and sodium 3,3′-(2-nonyl-1,3-dioxane-5,5-diyl)bis(methylene)bis(oxy)dipropane-1-sulfonate (SNDPS), have been studied by means of fluorescence spectroscopy and thermodynamic analysis. The fluorescence of BSA is quenched via a static quenching mechanism with the addition of the surfactants. The binding constants of the surfactants and proteins have been measured, with KA(SNPS) = 8.71×104 M−1 and KA(SNDPS) = 7.08 × 104 M−1, respectively. The interaction between surfactants and BSA is mainly of hydrophobic nature, based on the number of binding sites, n[n(SNPS) = 1.57, n(SNDPS) = 1.47], and the thermodynamic relationship. These results suggest that SNPS and SNDPS could be effective protein denaturants for protein separation and analysis.

Interactions of anionic surfactants with poly(ethylene oxide) and bovine serum albumin polymers: effect of the counterion hydrophobicity

1999 ◽  
Vol 147 (1-2) ◽  
pp. 89-105 ◽  
Author(s):  
Dino Zanette ◽  
Cláudio F Lima ◽  
Ângelo A Ruzza ◽  
Alexanders T.N Belarmino ◽  
Sônia de F. Santos ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Ethylene Oxide ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Anionic Surfactants ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene

Competitive Process of Binding Between the Anionic Surfactants Sodium Dodecyl Sulfate and Sodium Cholate in Bovine Serum Albumin

2005 ◽  
Vol 229 (1) ◽  
pp. 208-216 ◽  
Author(s):  
Bianca Schweitzer ◽  
Arlindo C. Felippe ◽  
Alexandre Dal Bó ◽  
Edson Minatti ◽  
Dino Zanette
Keyword(s):  
Bovine Serum Albumin ◽  
Sodium Dodecyl Sulfate ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Anionic Surfactants ◽  
Sodium Dodecyl ◽  
Sodium Cholate ◽  
Competitive Process ◽  
Dodecyl Sulfate

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

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