Cell detachment and label-free cell sorting using modulated surface acoustic waves (SAWs) in droplet-based microfluidics

Lab on a Chip ◽  
2014 ◽  
Vol 14 (18) ◽  
pp. 3556 ◽  
Author(s):  
Adrien Bussonnière ◽  
Yannick Miron ◽  
Michaël Baudoin ◽  
Olivier Bou Matar ◽  
Michel Grandbois ◽  
...  
Keyword(s):  
Cell Sorting ◽  
Acoustic Waves ◽  
Surface Acoustic Waves ◽  
Free Cell ◽  
Cell Detachment ◽  
Label Free

A ‘smart’ aptamer-functionalized continuous label-free cell catch-transport-release system

2021 ◽  
Author(s):  
Bozhen Zhang ◽  
Canran Wang ◽  
Yingjie Du ◽  
Rebecca Paxton ◽  
Ximin He
Keyword(s):  
Biomedical Research ◽  
Cell Sorting ◽  
Free Cell ◽  
Label Free ◽  
Clinical Therapeutics ◽  
Release System ◽  
System P

Label-free cell sorting devices are of great significance for biomedical research and clinical therapeutics. However, current platforms for label-free cell sorting cannot achieve continuity and selectivity simultaneously, resulting in complex...

The potential of autofluorescence for the detection of single living cells for label-free cell sorting in microfluidic systems

2004 ◽  
Vol 25 (21-22) ◽  
pp. 3740-3745 ◽  
Author(s):  
Jurjen Emmelkamp ◽  
Floor Wolbers ◽  
Helene Andersson ◽  
Ralph S. DaCosta ◽  
Brian C. Wilson ◽  
...  
Keyword(s):  
Cell Sorting ◽  
Free Cell ◽  
Living Cells ◽  
Label Free ◽  
Microfluidic Systems ◽  
Single Living Cells ◽  
Single Living

scholarly journals Theoretical Framework of Radiation Force in Surface Acoustic Waves for Modulated Particle Sorting

2019 ◽  
Vol 63 (2) ◽  
pp. 77-84
Author(s):  
Gergely Simon ◽  
Marco A. B. Andrade ◽  
Marc P. Y. Desmulliez ◽  
Mathis O. Riehle ◽  
Anne L. Bernassau
Keyword(s):  
Acoustic Waves ◽  
Acoustic Radiation ◽  
Surface Acoustic Waves ◽  
Radiation Force ◽  
Disease Diagnosis ◽  
Target Cells ◽  
Periodic Signal ◽  
Label Free ◽  
Non Invasive ◽  
Sorting Technique

Sorting specific target entities from sample mixtures is commonly used in many macroscale laboratory processing, such as disease diagnosis or treatment. Downscaling of sorting systems enables less laboratory space and fewer quantities of sample and reagent. Such lab-on-a-chip devices can perform separation functions using passive or active sorting methods. Such a method, acoustic sorting, when used in microfluidics, offers contactless, label-free, non-invasive manipulation of target cells or particles and is therefore the topic of active current research. Our phase-modulated sorting technique complements traditional time-of-flight techniques and offers higher sensitivity separation using a periodic signal. By cycling of this periodic signal, the target entities are gradually displaced compared to the background debris, thereby achieving sorting. In this paper, we extend the knowledge on phase-modulated sorting techniques. Firstly, using numerical simulations, we confirm the sorting role of our proposed primary acoustic radiation force within surface wave devices. Secondly, a threefold agreement between analytical, numerical and experimental sorting trajectories is presented.

Ultrafast chemical-free cell lysis by high speed stream collision induced by surface acoustic waves

2017 ◽  
Vol 110 (14) ◽  
pp. 143504 ◽  
Author(s):  
Wenbo Wang ◽  
Yishan Chen ◽  
Umar Farooq ◽  
Weipeng Xuan ◽  
Hao Jin ◽  
...  
Keyword(s):  
Acoustic Waves ◽  
High Speed ◽  
Surface Acoustic Waves ◽  
Cell Lysis ◽  
Free Cell ◽  
High Speed Stream

Modeling Development of a High-Sensitivity Escherichia coli O157:H7 Detection Based on SH SAW Sensor

2014 ◽  
Vol 925 ◽  
pp. 595-599
Author(s):  
Seng Teik Ten ◽  
Uda Hashim ◽  
Ahmad Sudin ◽  
Wei Wen Liu ◽  
Kai Loong Foo ◽  
...  
Keyword(s):  
Acoustic Waves ◽  
High Performance ◽  
Surface Acoustic Waves ◽  
Equivalent Circuit Model ◽  
High Sensitivity ◽  
Theoretical Models ◽  
Acoustic Energy ◽  
Sensitive Detection ◽  
Label Free ◽  
Element Analysis

Surface acoustic waves based devices were initially developed for the telecommunication purpose such as signal filters and resonators. The acoustic energy is strongly confined on the surface of the surface acoustic waves (SAW) based devices and consequent their ultra-sensitivity to the surface perturbation. This has made SAW permits the highly sensitive detection of utterly diminutive charges on the surface. Hence, SAW based devices have been modified to be sensors. Food contamination has become critical issue and sensitive detection devices are needed urgently as small amount of harmful bacterial pathogens such as Escherichia coli (E.coli) O157:H7with the dose fewer than 100 organisms in food products or water is enough to cause serious gastrointestinal illness to human. Therefore, ultra-high sensitive, label free biosensors have been designed in this research for the low concentration E.coli detection. After the saturated development in telecommunication filed, SAW sensors were developed for gas detections and have been moving towards biological detections recently. Shear horizontal surface acoustic wave (SHSAW), one of the SAW based types is most suitable for the liquid based application as it has the advantage of acoustic energy is not being radiated into liquid. Therefore, SHSAW device has the potential to provide high-performance sensing platform in this research. There have been a lot of complicated theoretical models for the SAW devices development since 1960 as signal filters and resonators such as from delta function model, equivalent circuit model, to the current SAW models such as coupling-of-modes (COM) model, P-matrix model and finite element analysis (FEA) model. However, SHSAW device in this research is not meant for signal filter or resonators but used for surface sensing purpose, therefore the simplicity method of the modeling is presented in the paper for the E.coli detection sensor development.

scholarly journals Using real-time fluorescence and deformability cytometry and deep learning to transfer molecular specificity to label-free sorting

2019 ◽  
Author(s):  
Ahmad Ahsan Nawaz ◽  
Marta Urbanska ◽  
Maik Herbig ◽  
Martin Nötzel ◽  
Martin Kräter ◽  
...  
Keyword(s):  
Real Time ◽  
Acoustic Waves ◽  
Blood Cells ◽  
Surface Acoustic Waves ◽  
Microfluidic Channel ◽  
Cell Types ◽  
Biological Research ◽  
Label Free ◽  
Molecular Specificity ◽  
Deformability Cytometry

The identification and separation of specific cells from heterogeneous populations is an essential prerequisite for further analysis or use. Conventional passive and active separation approaches rely on fluorescent or magnetic tags introduced to the cells of interest through molecular markers. Such labeling is time- and cost-intensive, can alter cellular properties, and might be incompatible with subsequent use, for example, in transplantation. Alternative label-free approaches utilizing morphological or mechanical features are attractive, but lack molecular specificity. Here we combine image-based real-time fluorescence and deformability cytometry (RT-FDC) with downstream cell sorting using standing surface acoustic waves (SSAW). We demonstrate basic sorting capabilities of the device by separating cell mimics and blood cell types based on fluorescence as well as deformability and other image parameters. The identification of blood sub-populations is enhanced by flow alignment and deformation of cells in the microfluidic channel constriction. In addition, the classification of blood cells using established fluorescence-based markers provides hundreds of thousands of labeled cell images used to train a deep neural network. The trained algorithm, with latency optimized to below 1 ms, is then used to identify and sort unlabeled blood cells at rates of 100 cells/sec. This approach transfers molecular specificity into label-free sorting and opens up new possibilities for basic biological research and clinical therapeutic applications.

Shear Horizontal Surface Acoustic Waves Sensor for Label-Free DNA Detection

2010 ◽  
Vol 8 (6) ◽  
pp. 813-817 ◽  
Author(s):  
C. Zerrouki ◽  
N. Fourati ◽  
M. Lazerges ◽  
J. M. Fougnion ◽  
C. Vedrine ◽  
...  
Keyword(s):  
Acoustic Waves ◽  
Dna Detection ◽  
Surface Acoustic Waves ◽  
Horizontal Surface ◽  
Label Free ◽  
Free Dna ◽  
Shear Horizontal
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