scholarly journals A nano-sized Cu-MOF with high peroxidase-like activity and its potential application in colorimetric detection of H2O2 and glucose

RSC Advances ◽  
2021 ◽  
Vol 11 (43) ◽  
pp. 26963-26973
Author(s):  
Hao Yu ◽  
Hanliu Wu ◽  
Xuemei Tian ◽  
Yafen Zhou ◽  
Chunguang Ren ◽  
...  

A Cu-MOF with high peroxidase-like activity was prepared and could be used for colorimetric detection of H2O2 and glucose with high selectivity and good linear range (50–500 μM).

Author(s):  
Karthikeyan Kandasamy ◽  
Sivaprasad Ganesabaskaran ◽  
Muthusamy Poomalai Pachamuthu ◽  
Rajamanickam Maheswari ◽  
Anand Ramanathan

RSC Advances ◽  
2016 ◽  
Vol 6 (20) ◽  
pp. 16285-16291 ◽  
Author(s):  
Le Chang ◽  
Adrian Fisher ◽  
Zhiping Liu ◽  
Daojian Cheng

For the colorimetric detection of S, SH, cysteine, and H2S, the sensitivity of Ag–Au nanoalloy is better than pure Ag cluster, and both of them possess high selectivity for the detection of S.


1999 ◽  
Vol 37 (9) ◽  
pp. 2793-2797 ◽  
Author(s):  
Ulrika Noborg ◽  
Annkatrin Gusdal ◽  
Eva K. Pisa ◽  
Anders Hedrum ◽  
Magnus Lindh

A highly sensitive method of quantitative analysis of hepatitis B virus (HBV) DNA in serum, the Cobas Amplicor HBV Monitor (Cobas-AM) test, was evaluated. Following a manual extraction of viral DNA, amplification, colorimetric detection, and quantitative determination are all automatically performed in the Cobas analyzer. Serially diluted samples with known HBV DNA concentrations were analyzed blindly. All samples with a virus concentration of 400 copies/ml and 83% of samples with a virus concentration of 100 copies/ml could be detected. A linear correlation between input HBV DNA and measured HBV DNA was seen in the range from 100 to 105 copies/ml. The mean coefficient of variation was 29.6% for all input levels and 18.9% for HBV DNA concentrations above 400 copies/ml. Samples with an HBV DNA level above 109 copies/ml could be reproducibly measured after predilution to 10−4 or 10−6 in negative serum; however, the level was underestimated if target DNA after dilution was still above the linear range of the assay. Quantitative results of the Cobas-AM test were interchangeable with measurements by the manual microwell plate version of Amplicor HBV Monitor (MWP-AM); the mean ratio for log Cobas-AM results/log MWP-AM results was 0.97 (standard error of the mean, 0.007) when serum samples from 153 chronic carriers were analyzed. The test should be of value for clinical assessment of chronic carriers and for monitoring the response to antiviral treatment. A limitation is the relatively narrow linear range of the assay, requiring predilution of high-titer (mainly hepatitis B e-antigen-positive) samples.


The Analyst ◽  
2019 ◽  
Vol 144 (15) ◽  
pp. 4582-4588 ◽  
Author(s):  
Wei Duan ◽  
Ao Liu ◽  
Qing Li ◽  
Zhiwei Li ◽  
Cong-ying Wen ◽  
...  

A colorimetric method for detecting formaldehyde was developed by coupling bone-shaped gold nanorods (AuNRs) with silver mirror reaction, which enables low detection limit, wide linear range and high visual resolution.


The Analyst ◽  
2019 ◽  
Vol 144 (6) ◽  
pp. 1948-1954 ◽  
Author(s):  
Xin Gong ◽  
Xiao Li ◽  
Taiping Qing ◽  
Peng Zhang ◽  
Bo Feng

An indirect competitive enzyme-linked aptamer assay (ELAA) with multivalent HRP-mimicking DNAzyme for colorimetric detection of tetracycline. Aptamer modified multivalent HRP-mimicking DNAzyme exhibited high selectivity and enhanced catalytic capacity, which improved the detection sensitivity greatly.


Polymers ◽  
2018 ◽  
Vol 10 (7) ◽  
pp. 786 ◽  
Author(s):  
Rui Jiang ◽  
Na Liu ◽  
Fan Li ◽  
Wensheng Fu ◽  
Yun Zhou ◽  
...  

Herein, a novel on-off-on fluorescent chemosensor for copper (II) ion (Cu2+), iron (III) ion (Fe3+) and cysteine is developed simply by the nano-precipitation method. The prepared organic dots with AIEgens (AIE dots) are advantageous over other metal ions in detecting Cu2+, Fe3+ with high selectivity and sensitivity by forming agglomerations (on-off). The agglomerations formed by AIE dots and Cu2+ redistributed and the fluorescence was obviously recovered in the presence of cysteine (off-on). This sensor has a wide linear range for Cu2+, Fe3+ and cysteine. The fluorescent detection limits of AIE dots are calculated to be 107 nM for Cu2+, 120 nM for Fe3+ and 78 nM for cysteine, respectively. These results indicate that the AIE dots can be used as a potential probe for Cu2+, Fe3+ and cysteine detection.


Biomolecules ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 647
Author(s):  
Kai Liu ◽  
Yu Zhao ◽  
Lu Zhang ◽  
Mengmeng He ◽  
Weifeng Lin ◽  
...  

Ascorbic acid is an organic compound with antioxidant properties that can protect the human body from the threat of free radicals. Therefore, it is important to detect the existence and measure the concentration of ascorbic acid to regulate its content in the human body. In this work, we prepared bitter gourd polysaccharide (BGP)-stabilized platinum nanoclusters (Pt-BGP NCs) by reacting BGP with K2PtCl4. Pt-BGP NCs and catalyzed the decomposition of H2O2 to generate •OH radicals, which could oxidize TMB to form oxidized TMB (oxTMB), indicating their peroxidase-like properties. The kinetics followed the Michaelis–Menten equation. Furthermore, the colorimetric detection of ascorbic acid using Pt-BGP NCs showed high selectivity and a low detection limit of 0.191 μM. The accuracy of real sample detection using Pt-BGP NCs was as high as 98.9%. More importantly, Pt-BGP NCs had high cell biocompatibility and extremely low hemolysis rate due to the component of BGP. In summary, the prepared Pt-BGP NCs with reductive activity and good biocompatibility have good application prospects in colorimetric detection of ascorbic acid.


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