Amplified colorimetric detection of tetracycline based on an enzyme-linked aptamer assay with multivalent HRP-mimicking DNAzyme

The Analyst ◽  
2019 ◽  
Vol 144 (6) ◽  
pp. 1948-1954 ◽  
Author(s):  
Xin Gong ◽  
Xiao Li ◽  
Taiping Qing ◽  
Peng Zhang ◽  
Bo Feng
Keyword(s):  
High Selectivity ◽  
Colorimetric Detection ◽  
Detection Sensitivity ◽  
Aptamer Assay ◽  
Catalytic Capacity

An indirect competitive enzyme-linked aptamer assay (ELAA) with multivalent HRP-mimicking DNAzyme for colorimetric detection of tetracycline. Aptamer modified multivalent HRP-mimicking DNAzyme exhibited high selectivity and enhanced catalytic capacity, which improved the detection sensitivity greatly.

Highly sensitive and selective colorimetric detection of sulphide using Ag–Au nanoalloys: a DFT study

RSC Advances ◽  
2016 ◽  
Vol 6 (20) ◽  
pp. 16285-16291 ◽  
Author(s):  
Le Chang ◽  
Adrian Fisher ◽  
Zhiping Liu ◽  
Daojian Cheng
Keyword(s):  
High Selectivity ◽  
Colorimetric Detection ◽  
Dft Study ◽  
Highly Sensitive ◽  
Ag Cluster ◽  
Better Than

For the colorimetric detection of S, SH, cysteine, and H2S, the sensitivity of Ag–Au nanoalloy is better than pure Ag cluster, and both of them possess high selectivity for the detection of S.

A novel potentiometric sensor based on urease/ bovine serum albumin-poly(3,4-ethylenedioxythiophene)/Pt for urea detection

2016 ◽  
Vol 71 (4) ◽  
pp. 277-282 ◽  
Author(s):  
Chien-Hsing Hsu ◽  
Ya-Wei Hsu ◽  
Yu-Ching Weng
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
High Selectivity ◽  
Ph Value ◽  
Detection Sensitivity ◽  
Urea Solution ◽  
Pt Electrode ◽  
Amide Bonds ◽  
Order Of Magnitude

AbstractWe have presented a potentiometric urea sensor using an urease/bovine serum albumin (BSA)-poly(3,4-ethylenedioxythiophene)(PEDOT)/Pt electrode. A urea detection sensitivity of 15.2 mV/decade (order of magnitude) has been achieved. BSA trapped in the PEDOT matrix was employed to bond urease molecules on the surface of a BSA-PEDOT/Pt electrode via amide bonds formed between the carboxyl functional groups on the enzyme and the amines on the BSA. The effects of PEDOT thickness, pH value of the urea solution, urease concentration, and temperature on the urea detection sensitivity were also studied. The lifetime of the sensor was studied for a period of 10 weeks, and the average sensing degradation rate was about 9 % per week. This sensor displayed a high selectivity to urea over glucose, KCl, and NaCl.

scholarly journals A nano-sized Cu-MOF with high peroxidase-like activity and its potential application in colorimetric detection of H2O2 and glucose

RSC Advances ◽  
2021 ◽  
Vol 11 (43) ◽  
pp. 26963-26973
Author(s):  
Hao Yu ◽  
Hanliu Wu ◽  
Xuemei Tian ◽  
Yafen Zhou ◽  
Chunguang Ren ◽  
...  
Keyword(s):  
Potential Application ◽  
High Selectivity ◽  
Colorimetric Detection ◽  
Linear Range ◽  
Good Linear

A Cu-MOF with high peroxidase-like activity was prepared and could be used for colorimetric detection of H2O2 and glucose with high selectivity and good linear range (50–500 μM).

scholarly journals Image Digitization of Colorimetric Detection of Acephate Based on Its Complexation with Citrate-Capped Gold Nanoparticles

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Mohd Junaedy Osman ◽  
Wan Md Zin Wan Yunus ◽  
Keat Khim Ong ◽  
Buong Woei Chieng ◽  
Noor Azilah Mohd Kassim ◽  
...  
Keyword(s):  
Image Processing ◽  
Gold Nanoparticles ◽  
Limit Of Detection ◽  
Colorimetric Detection ◽  
Linear Regression Analysis ◽  
Processing Technique ◽  
Detection Sensitivity ◽  
Image Processing Technique ◽  
Detection Accuracy ◽  
Colour Image

Acephate (Ac), an organophosphate (OP) insecticide, is very harmful to human and the environment. Conventional techniques of detection are sensitive and selective but relatively time-consuming, expensive, and require trained personnel. This paper describes the use of an image processing technique to digitize the red values (RVs) of the colour image of Ac-capped gold nanoparticles (Ac-Cit-AuNPs) complex captured using a digital microscope to improve the detection accuracy and precision. The formation of the suspension was characterised using laser test, ultraviolet-visible spectrophotometer (UV-Vis), high-resolution transmission electron microscope (HRTEM), and Fourier Transform infrared spectroscope (FTIR). The linear regression analysis revealed that the detection sensitivity improved as the smaller gold nanoparticles were used. For quantitative measurement using image processing, a good linear relationship (R2 = 0.9905 and 0.9924) for Cit-HAuNPs and Cit-MAuNPs, respectively, between the concentration of Ac and average red values was obtained in the range of 0–8 mM. The limit of detection (LOD) for Ac was found to be 0.3 mM and 0.4 mM for Cit-MAuNPs and Cit-HAuNPs, respectively.

scholarly journals Biocompatible Platinum Nanoclusters Prepared Using Bitter Gourd Polysaccharide for Colorimetric Detection of Ascorbic Acid

Biomolecules ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 647
Author(s):  
Kai Liu ◽  
Yu Zhao ◽  
Lu Zhang ◽  
Mengmeng He ◽  
Weifeng Lin ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Human Body ◽  
High Selectivity ◽  
Colorimetric Detection ◽  
Antioxidant Properties ◽  
Bitter Gourd ◽  
Oh Radicals ◽  
High Cell ◽  
Good Biocompatibility ◽  
Platinum Nanoclusters

Ascorbic acid is an organic compound with antioxidant properties that can protect the human body from the threat of free radicals. Therefore, it is important to detect the existence and measure the concentration of ascorbic acid to regulate its content in the human body. In this work, we prepared bitter gourd polysaccharide (BGP)-stabilized platinum nanoclusters (Pt-BGP NCs) by reacting BGP with K2PtCl4. Pt-BGP NCs and catalyzed the decomposition of H2O2 to generate •OH radicals, which could oxidize TMB to form oxidized TMB (oxTMB), indicating their peroxidase-like properties. The kinetics followed the Michaelis–Menten equation. Furthermore, the colorimetric detection of ascorbic acid using Pt-BGP NCs showed high selectivity and a low detection limit of 0.191 μM. The accuracy of real sample detection using Pt-BGP NCs was as high as 98.9%. More importantly, Pt-BGP NCs had high cell biocompatibility and extremely low hemolysis rate due to the component of BGP. In summary, the prepared Pt-BGP NCs with reductive activity and good biocompatibility have good application prospects in colorimetric detection of ascorbic acid.

Colorimetric detection of cysteine based on dispersion–aggregation mechanism of chitosan stabilized gold nanoparticles

2019 ◽  
Vol 97 (3) ◽  
pp. 233-237 ◽  
Author(s):  
Esther Jeyasekaran ◽  
Sridevi Venkatachalam
Keyword(s):  
Gold Nanoparticles ◽  
High Selectivity ◽  
Limit Of Detection ◽  
Colorimetric Detection ◽  
Colorimetric Method ◽  
Colour Change ◽  
Selective Detection ◽  
Aggregation Mechanism ◽  
Plausible Mechanism ◽  
Sulfur Containing

A simple and selective colorimetric method for detection of cysteine using chitosan stabilized gold nanoparticles has been developed. L-cysteine is a sulfur containing amino acid. Cysteine binds with the gold nanoparticles through a sulfur group and induces aggregation of the nanoparticles, which leads to a visible colour change from pale red to blue. A good linearity was observed from 0.1 to 30 μmol/L (R = 0.9958) cysteine, and the limit of detection was found to be 0.1 μmol/L. The gold nanoparticles showed a high selectivity towards the detection of cysteine even in the presence of 10 000 fold higher concentration of common interferences such as glutathione, methionine, and homocysteine. A plausible mechanism for the selective detection of cysteine in presence of interferences based on a dispersion–aggregation mechanism has been proposed.

scholarly journals Facile fabrication of paper-based analytical devices for rapid and highly selective colorimetric detection of cesium in environmental samples

RSC Advances ◽  
2017 ◽  
Vol 7 (76) ◽  
pp. 48374-48385 ◽  
Author(s):  
Sung-Min Kang ◽  
Sung-Chan Jang ◽  
Yuvaraj Haldorai ◽  
A. T. Ezhil Vilian ◽  
Muruganantham Rethinasabapathy ◽  
...  
Keyword(s):  
Human Health ◽  
Environmental Samples ◽  
High Selectivity ◽  
Colorimetric Detection ◽  
Detection Capability ◽  
Radioactive Contaminant ◽  
Facile Fabrication

Cesium (Cs), a radioactive contaminant of the ecosystem, causes a major risk to human health and environments. This chemo-indicator is designed to exhibit a powerful detection capability featuring high selectivity and sensitivity to inactive Cs.

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