Membrane-curvature-mediated co-endocytosis of bystander and functional nanoparticles

Nanoscale ◽  
2021 ◽  
Author(s):  
Kejie He ◽  
Yushuang Wei ◽  
Zhihong Zhang ◽  
Haibo Chen ◽  
Bing Yuan ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Biomedical Applications ◽  
Membrane Curvature ◽  
Functional Nanoparticles

Efficient cellular uptake of nanoparticles (NPs) is necessary for the development of nanomedicine in biomedical applications. Recently, the coadministration of functionalized NPs (FNPs) was shown to stimulate the cellular uptake...

Pre-coating layered double hydroxide nanoparticles with albumin to improve colloidal stability and cellular uptake

2015 ◽  
Vol 3 (16) ◽  
pp. 3331-3339 ◽  
Author(s):  
Zi Gu ◽  
Huali Zuo ◽  
Li Li ◽  
Aihua Wu ◽  
Zhi Ping Xu
Keyword(s):  
Layered Double Hydroxide ◽  
Cellular Uptake ◽  
Biomedical Applications ◽  
Colloidal Stability ◽  
New Strategy ◽  
Double Hydroxide ◽  
Layered Double Hydroxide Nanoparticles

We introduced a new strategy of albumin pre-coating to effectively stabilise layered double hydroxide (LDH) nanoparticles for biomedical applications.

scholarly journals Bioaerogels: Synthesis approaches, cellular uptake, and the biomedical applications

2019 ◽  
Vol 111 ◽  
pp. 964-975 ◽  
Author(s):  
Fatemeh Pashaei Soorbaghi ◽  
Mojgan Isanejad ◽  
Sara Salatin ◽  
Milad Ghorbani ◽  
Samira Jafari ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Biomedical Applications

scholarly journals Systematic Evaluation of Light-Activatable Biohybrids for Anti-Glioma Photodynamic Therapy

2019 ◽  
Vol 8 (9) ◽  
pp. 1269 ◽  
Author(s):  
Inglut ◽  
Baglo ◽  
Liang ◽  
Cheema ◽  
Stabile ◽  
...  
Keyword(s):  
Cellular Uptake ◽  
Biomedical Applications ◽  
Biological Properties ◽  
Systematic Evaluation ◽  
Therapeutic Effects ◽  
End Effects ◽  
Fundamental Understanding ◽  
Effective Use ◽  
Oxygen Yield ◽  
New Generation

Photosensitizing biomolecules (PSBM) represent a new generation of light-absorbing compounds with improved optical and physicochemical properties for biomedical applications. Despite numerous advances in lipid-, polymer-, and protein-based PSBMs, their effective use requires a fundamental understanding of how macromolecular structure influences the physicochemical and biological properties of the photosensitizer. Here, we prepared and characterized three well-defined PSBMs based on a clinically used photosensitizer, benzoporphyrin derivative (BPD). The PSBMs include 16:0 lysophosphocholine-BPD (16:0 Lyso PC-BPD), distearoyl-phosphoethanolamine-polyethylene-glycol-BPD (DSPE-PEG-BPD), and anti-EGFR cetuximab-BPD (Cet-BPD). In two glioma cell lines, DSPE-PEG-BPD exhibited the highest singlet oxygen yield but was the least phototoxic due to low cellular uptake. The 16:0 Lyso PC-BPD was most efficient in promoting cellular uptake but redirected BPD’s subcellular localization from mitochondria to lysosomes. At 24 h after incubation, proteolyzed Cet-BPD was localized to mitochondria and effectively disrupted the mitochondrial membrane potential upon light activation. Our results revealed the variable trafficking and end effects of PSBMs, providing valuable insights into methods of PSBM evaluation, as well as strategies to select PSBMs based on subcellular targets and cytotoxic mechanisms. We demonstrated that biologically informed combinations of PSBMs to target lysosomes and mitochondria, concurrently, may lead to enhanced therapeutic effects against gliomas.

Functional nanoparticles for biomedical applications

Nanoscale ◽  
2013 ◽  
Vol 5 (23) ◽  
pp. 11338 ◽  
Author(s):  
Nguyễn Thi Kim Thanh
Keyword(s):  
Biomedical Applications ◽  
Functional Nanoparticles

Controllable synthesis of functional nanoparticles by microfluidic platforms for biomedical applications – a review

Lab on a Chip ◽  
2017 ◽  
Vol 17 (2) ◽  
pp. 209-226 ◽  
Author(s):  
Junping Ma ◽  
Simon Ming-Yuen Lee ◽  
Changqing Yi ◽  
Cheuk-Wing Li
Keyword(s):  
Drug Delivery ◽  
Biomedical Applications ◽  
Controllable Synthesis ◽  
Microfluidic Systems ◽  
Microfluidic Platforms ◽  
Functional Nanoparticles

This review summarizes the development of microfluidic systems for engineering nanoparticles and their applications in imaging, biosensing, drug delivery and theranostics.

scholarly journals Carbon Nano-Onions as Non-Cytotoxic Carriers for Cellular Uptake of Glycopeptides and Proteins

Nanomaterials ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 1069 ◽  
Author(s):  
Marta d’Amora ◽  
Viviana Maffeis ◽  
Rosaria Brescia ◽  
Danielle Barnes ◽  
Eoin Scanlan ◽  
...  
Keyword(s):  
Surface Modification ◽  
Bovine Serum Albumin ◽  
Cellular Uptake ◽  
Bovine Serum ◽  
Biomedical Applications ◽  
Addition Reaction ◽  
Covalent Immobilization ◽  
Endocytosis Pathway ◽  
Good Biocompatibility ◽  
Protein Bovine Serum Albumin

Carbon nano-onions (CNOs) possess favorable properties that make them suitable for biomedical applications, including their small size, ready surface modification, and good biocompatibility. Here, we report the covalent immobilization of a synthetic glycopeptide and the protein bovine serum albumin (BSA) onto the surface of carbon nano-onions using the maleimide–thiol “addition reaction”. The glycopeptide and BSA are readily transported inside different cell lines, together with carbon nano-onions, through the endocytosis pathway. Our results show that carbon nano-onions are excellent scaffolds for glycopeptides and proteins immobilization and act as intracellular carriers for these biomolecules. These findings open new perspectives in the application of carbon nano-onions as intracellular transporters in diverse biomedical applications.

scholarly journals g-force induced giant efficiency of nanoparticles internalization into living cells

2015 ◽  
Vol 5 (1) ◽  
Author(s):  
Sandra M. Ocampo ◽  
Vanessa Rodriguez ◽  
Leonor de la Cueva ◽  
Gorka Salas ◽  
Jose. L. Carrascosa ◽  
...  
Keyword(s):  
Clinical Trials ◽  
Cellular Uptake ◽  
Biomedical Applications ◽  
Human Mesenchymal Stem Cells ◽  
Living Cells ◽  
Oxide Nanoparticles ◽  
Preclinical Studies ◽  
High Uptake ◽  
Electrical Fields ◽  
Endocytosis Pathway

Abstract Nanotechnology plays an increasingly important role in the biomedical arena. Iron oxide nanoparticles (IONPs)-labelled cells is one of the most promising approaches for a fast and reliable evaluation of grafted cells in both preclinical studies and clinical trials. Current procedures to label living cells with IONPs are based on direct incubation or physical approaches based on magnetic or electrical fields, which always display very low cellular uptake efficiencies. Here we show that centrifugation-mediated internalization (CMI) promotes a high uptake of IONPs in glioblastoma tumour cells, just in a few minutes and via clathrin-independent endocytosis pathway. CMI results in controllable cellular uptake efficiencies at least three orders of magnitude larger than current procedures. Similar trends are found in human mesenchymal stem cells, thereby demonstrating the general feasibility of the methodology, which is easily transferable to any laboratory with great potential for the development of improved biomedical applications.

Recent Advance in Synthesis and Application of Inorganic Glyconanoparticles as Bio-Therapeutics and Diagnostics Agents

2013 ◽  
Vol 754 ◽  
pp. 1-19
Author(s):  
Udayanath Aich
Keyword(s):  
Drug Discovery ◽  
Cellular Uptake ◽  
Biomedical Applications ◽  
Biomedical Application ◽  
Inorganic Nanoparticles ◽  
Biological Applications ◽  
Drug Candidates ◽  
The Poor ◽  
Serum Clearance ◽  
High Concentrations

Carbohydrates are attractive molecules for drug discovery because sugars are involved in many intricate human diseases including cancer and infectious diseases. Potential therapeutic and diagnostic benefits of sugar-based drugs, however, are offset by the poor pharmacologic properties of these molecules that include speedy serum clearance, poor cellular uptake, and the relatively high concentrations required for efficacy. To address these issues, carbohydrates are functionalized with nanocarrier as similar to peptides, proteins and DNA. Considering the vast relevance of Inorganic nanoparticles as promising candidates for electronic, optical, magnetic and biomedical applications, several metals linked glyconanoparticles (GNPs) are synthesized and applied for biomedical application. This article will elaborately discuss about the progress in the development of metallic GNPs for various biological applications as drug candidates and detection agents.

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