scholarly journals Spontaneous DNA Translocation Through van der Waals Heterostructure Nanopore for Single-Molecule Detection

2021 ◽  
Author(s):  
Yang Liu ◽  
Ye Deng ◽  
Yanmei Yang ◽  
Yuanyuan Qu ◽  
Chao Zhang ◽  
...  
Keyword(s):  
Solid State ◽  
High Precision ◽  
High Throughput ◽  
Single Molecule ◽  
Van Der Waals ◽  
Single Molecule Detection ◽  
Dna Translocation ◽  
New Paradigm ◽  
Van Der Waals Heterostructure ◽  
Long Read

Solid-state nanopore detection and sequencing of single-molecule offers a new paradigm because of several well-recognized features like long-read, high throughput, high precision and direct analyses. However, several key technical challenges...

Highly Sensitive Detection of Paraquat with Pillar[5]arene as an aptamer in α-Hemolysin Nanopore

2021 ◽  
Author(s):  
Xiaojia Jiang ◽  
Mingsong Zang ◽  
Fei Li ◽  
Chunxi Hou ◽  
Quan Luo ◽  
...  
Keyword(s):  
Real Time ◽  
High Throughput ◽  
Single Molecule ◽  
Single Molecule Detection ◽  
Sensitive Detection ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
The Real ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Biological nanopore-based techniques have attracted more and more attention recently in the field of single-molecule detection, because they allow the real-time, sensitive, high-throughput analysis. Herein, we report an engineered biological...

scholarly journals Parallel, linear, and subnanometric 3D tracking of microparticles with Stereo Darkfield Interferometry

2021 ◽  
Vol 7 (6) ◽  
pp. eabe3902
Author(s):  
Martin Rieu ◽  
Thibault Vieille ◽  
Gaël Radou ◽  
Raphaël Jeanneret ◽  
Nadia Ruiz-Gutierrez ◽  
...  
Keyword(s):  
High Precision ◽  
High Throughput ◽  
Single Molecule ◽  
Particle Tracking ◽  
Focal Plane ◽  
Tracking System ◽  
Three Dimensional ◽  
3D Tracking ◽  
Single Molecule Force Spectroscopy ◽  
A New Technique

While crucial for force spectroscopists and microbiologists, three-dimensional (3D) particle tracking suffers from either poor precision, complex calibration, or the need of expensive hardware, preventing its massive adoption. We introduce a new technique, based on a simple piece of cardboard inserted in the objective focal plane, that enables simple 3D tracking of dilute microparticles while offering subnanometer frame-to-frame precision in all directions. Its linearity alleviates calibration procedures, while the interferometric pattern enhances precision. We illustrate its utility in single-molecule force spectroscopy and single-algae motility analysis. As with any technique based on back focal plane engineering, it may be directly embedded in a commercial objective, providing a means to convert any preexisting optical setup in a 3D tracking system. Thanks to its precision, its simplicity, and its versatility, we envision that the technique has the potential to enhance the spreading of high-precision and high-throughput 3D tracking.

Measuring Trapped DNA at the Liquid-Air Interface for Enhanced Single Molecule Sensing

Nanoscale ◽  
2021 ◽  
Author(s):  
Nasim Farajpour ◽  
Lauren Lastra ◽  
Vinay Sharma ◽  
Kevin Freedman
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Borosilicate Glass ◽  
Low Noise ◽  
Viable Alternative ◽  
Single Molecule Detection ◽  
Widespread Application ◽  
Promising Tool ◽  
Air Interface ◽  
Cost Efficient

Nanopore sensing is a promising tool with widespread application in single-molecule detection. Borosilicate glass nanopores are a viable alternative to other solid-state nanopores due to low noise and cost-efficient fabrication....

SEM-induced shrinking of solid-state nanopores for single molecule detection

2011 ◽  
Vol 22 (42) ◽  
pp. 425302 ◽  
Author(s):  
Anmiv S Prabhu ◽  
Kevin J Freedman ◽  
Joseph W F Robertson ◽  
Zhorro Nikolov ◽  
John J Kasianowicz ◽  
...  
Keyword(s):  
Solid State ◽  
Single Molecule ◽  
Single Molecule Detection

Shrinking Graphene Nanopore Using Electron-Beam-Induced Deposition for Single Molecule Detection

2015 ◽  
Author(s):  
Jian Ma ◽  
Weiwei Zhao ◽  
Lei Liu ◽  
Jingjie Sha ◽  
Yunfei Chen
Keyword(s):  
Electron Beam ◽  
Electron Microscope ◽  
Solid State ◽  
Single Molecule ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Single Molecule Detection ◽  
Large Size ◽  
Scanning Electron ◽  
Graphene Nanopore

Solid-state nanopore has already shown success of single molecule detection and graphene nanopore is potential for successful DNA sequencing. Here, we present a fast and controllable way to fabricate sub-5 nm nanopore on graphene membrane. The process includes two steps: sputtering a large size nanopore using a conventional focused ion beam (FIB) and shrinking the large nanopore to a few nanometers using scanning electron microscope (SEM). We also demonstrated the ability of the graphene nanopores fabricated in this manner to detect individual 48Kbp λ-DNA molecules.

Single-Molecule Detection Technologies in Miniaturized High-Throughput Screening: Fluorescence Intensity Distribution Analysis

2003 ◽  
Vol 8 (1) ◽  
pp. 19-33 ◽  
Author(s):  
Ulrich Haupts ◽  
Martin Rüdiger ◽  
Stephen Ashman ◽  
Sandra Turconi ◽  
Ryan Bingham ◽  
...  
Keyword(s):  
High Throughput ◽  
Single Molecule ◽  
Fluorescence Intensity ◽  
Intensity Distribution ◽  
High Throughput Screening ◽  
Biological Data ◽  
Single Molecule Detection ◽  
Distribution Analysis ◽  
Fluorescence Intensity Distribution Analysis ◽  
Detection Technologies

Single-molecule detection technologies are becoming a powerful readout format to support ultra-high-throughput screening. These methods are based on the analysis of fluorescence intensity fluctuations detected from a small confocal volume element. The fluctuating signal contains information about the mass and brightness of the different species in a mixture. The authors demonstrate a number of applications of fluorescence intensity distribution analysis (FIDA), which discriminates molecules by their specific brightness. Examples for assays based on brightness changes induced by quenching/dequenching of fluorescence, fluorescence energy transfer, and multiple-binding stoichiometry are given for important drug targets such as kinases and proteases. FIDA also provides a powerful method to extract correct biological data in the presence of compound fluorescence. ( Journal of Biomolecular Screening 2003:19-33)

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