scholarly journals A sample-to-answer electrochemical biosensor system for biomarker detection

Lab on a Chip ◽  
2022 ◽  
Author(s):  
Kruthika Kikkeri ◽  
Dan Wu ◽  
Joel Voldman
Keyword(s):  
Electrochemical Biosensor ◽  
Blood Collection ◽  
Biomarker Detection ◽  
Plasma Separation ◽  
Collection Device ◽  
Detection Assay ◽  
Biosensor System ◽  
On Chip ◽  
Detection Of Biomarkers

We interfaced with a painless blood collection device and integrated on-chip blood-to-plasma separation with an electronic bead-based biomarker detection assay to enable true sample-to-answer detection of biomarkers.

scholarly journals An integrated homogeneous SPARCL™ immunoassay for rapid biomarker detection on a chip

2019 ◽  
Vol 11 (19) ◽  
pp. 2542-2550 ◽  
Author(s):  
Natalia Sandetskaya ◽  
Nicole Isserstedt-John ◽  
Andreas Kölsch ◽  
Sebastian Schattschneider ◽  
Dirk Kuhlmeier
Keyword(s):  
Quantitative Detection ◽  
Biomarker Detection ◽  
On Chip ◽  
Detection Of Biomarkers

A very simple and rapid homogeneous SPARCL™ immunoassay with on-chip preserved dry reagents enables sensitive quantitative detection of biomarkers.

scholarly journals Clinical Evaluation of the Torq Zero Delay Centrifuge System for Decentralized Blood Collection and Stabilization

Diagnostics ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1019
Author(s):  
Kyungjin Hong ◽  
Gabriella Iacovetti ◽  
Ali Rahimian ◽  
Sean Hong ◽  
Jon Epperson ◽  
...  
Keyword(s):  
Clinical Chemistry ◽  
Blood Collection ◽  
Test Results ◽  
Sample Collection ◽  
Rapid Separation ◽  
Cell Counts ◽  
Collection Device ◽  
Precision Study ◽  
Clinically Significant ◽  
Blood Specimens

Blood sample collection and rapid separation—critical preanalytical steps in clinical chemistry—can be challenging in decentralized collection settings. To address this gap, the Torq™ zero delay centrifuge system includes a lightweight, hand-portable centrifuge (ZDrive™) and a disc-shaped blood collection device (ZDisc™) enabling immediate sample centrifugation at the point of collection. Here, we report results from clinical validation studies comparing performance of the Torq System with a conventional plasma separation tube (PST). Blood specimens from 134 subjects were collected and processed across three independent sites to compare ZDisc and PST performance in the assessment of 14 analytes (K, Na, Cl, Ca, BUN, creatinine, AST, ALT, ALP, total bilirubin, albumin, total protein, cholesterol, and triglycerides). A 31-subject precision study was performed to evaluate reproducibility of plasma test results from ZDiscs, and plasma quality was assessed by measuring hemolysis and blood cells from 10 subject specimens. The ZDisc successfully collected and processed samples from 134 subjects. ZDisc results agreed with reference PSTs for all 14 analytes with mean % biases well below clinically significant levels. Results were reproducible across different operators and ZDisc production lots, and plasma blood cell counts and hemolysis levels fell well below clinical acceptance thresholds. ZDiscs produce plasma samples equivalent to reference PSTs. Results support the suitability of the Torq System for remotely collecting and processing blood samples in decentralized settings.

scholarly journals Metal Nanoparticle and Quantum Dot Tags for Signal Amplification in Electrochemical Immunosensors for Biomarker Detection

Chemosensors ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 85
Author(s):  
Anton Popov ◽  
Benediktas Brasiunas ◽  
Asta Kausaite-Minkstimiene ◽  
Almira Ramanaviciene
Keyword(s):  
Signal Amplification ◽  
Electrochemical Immunosensor ◽  
Metal Nanoparticle ◽  
Biomarker Detection ◽  
Analytical Instruments ◽  
Electrochemical Immunosensors ◽  
Highly Sensitive ◽  
Recent Developments ◽  
Electrochemical Signal ◽  
Detection Of Biomarkers

With the increasing importance of healthcare and clinical diagnosis, as well as the growing demand for highly sensitive analytical instruments, immunosensors have received considerable attention. In this review, electrochemical immunosensor signal amplification strategies using metal nanoparticles (MNPs) and quantum dots (Qdots) as tags are overviewed, focusing on recent developments in the ultrasensitive detection of biomarkers. MNPs and Qdots can be used separately or in combination with other nanostructures, while performing the function of nanocarriers, electroactive labels, or catalysts. Thus, different functions of MNPs and Qdots as well as recent advances in electrochemical signal amplification are discussed. Additionally, the methods most often used for antibody immobilization on nanoparticles, immunoassay formats, and electrochemical methods for indirect biomarker detection are overviewed.

scholarly journals Recent Advances in Portable Biosensors for Biomarker Detection in Body Fluids

Biosensors ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 127 ◽  
Author(s):  
Brian Senf ◽  
Woon-Hong Yeo ◽  
Jong-Hoon Kim
Keyword(s):  
Minimally Invasive ◽  
Clinical Intervention ◽  
Body Fluids ◽  
Disease Spread ◽  
Biomarker Detection ◽  
Sample Collection ◽  
Less Invasive ◽  
Recent Advances ◽  
Detection Of Biomarkers

A recent development in portable biosensors allows rapid, accurate, and on-site detection of biomarkers, which helps to prevent disease spread by the control of sources. Less invasive sample collection is necessary to use portable biosensors in remote environments for accurate on-site diagnostics and testing. For non- or minimally invasive sampling, easily accessible body fluids, such as saliva, sweat, blood, or urine, have been utilized. It is also imperative to find accurate biomarkers to provide better clinical intervention and treatment at the onset of disease. At the same time, these reliable biomarkers can be utilized to monitor the progress of the disease. In this review, we summarize the most recent development of portable biosensors to detect various biomarkers accurately. In addition, we discuss ongoing issues and limitations of the existing systems and methods. Lastly, we present the key requirements of portable biosensors and discuss ideas for functional enhancements.

scholarly journals Programmable Paper-Based Microfluidic Devices for Biomarker Detections

Micromachines ◽  
2019 ◽  
Vol 10 (8) ◽  
pp. 516 ◽  
Author(s):  
Veasna Soum ◽  
Sooyong Park ◽  
Albertus Ivan Brilian ◽  
Oh-Sun Kwon ◽  
Kwanwoo Shin
Keyword(s):  
Point Of Care ◽  
High Sensitivity ◽  
Microfluidic Devices ◽  
Portable Devices ◽  
Biomarker Detection ◽  
Fluid Delivery ◽  
Wide Range ◽  
Paper Based Microfluidics ◽  
Digital Microfluidic ◽  
Detection Of Biomarkers

Recent advanced paper-based microfluidic devices provide an alternative technology for the detection of biomarkers by using affordable and portable devices for point-of-care testing (POCT). Programmable paper-based microfluidic devices enable a wide range of biomarker detection with high sensitivity and automation for single- and multi-step assays because they provide better control for manipulating fluid samples. In this review, we examine the advances in programmable microfluidics, i.e., paper-based continuous-flow microfluidic (p-CMF) devices and paper-based digital microfluidic (p-DMF) devices, for biomarker detection. First, we discuss the methods used to fabricate these two types of paper-based microfluidic devices and the strategies for programming fluid delivery and for droplet manipulation. Next, we discuss the use of these programmable paper-based devices for the single- and multi-step detection of biomarkers. Finally, we present the current limitations of paper-based microfluidics for biomarker detection and the outlook for their development.

scholarly journals Nanozyme-based electrochemical biosensors for disease biomarker detection

The Analyst ◽  
2020 ◽  
Vol 145 (13) ◽  
pp. 4398-4420 ◽  
Author(s):  
Rabbee G. Mahmudunnabi ◽  
Fatema Zerin Farhana ◽  
Navid Kashaninejad ◽  
Shakhawat H. Firoz ◽  
Yoon-Bo Shim ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Electrochemical Biosensors ◽  
Biomarker Detection ◽  
Disease Biomarker

In this review, we have summarised the synthesis and classification of common nanozymes and their applications in electrochemical biosensor development.

On-Line Elimination of Electroactive Interferents for Flow-Type Electrochemical Biosensor System

1991 ◽  
Vol 20 (5) ◽  
pp. 849-852 ◽  
Author(s):  
Yusuke Okawa ◽  
Hiroyuki Kobayashi ◽  
Takashi Ohno
Keyword(s):  
Electrochemical Biosensor ◽  
Flow Type ◽  
Biosensor System ◽  
On Line
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