scholarly journals A microfluidic device enabling high-efficiency single cell trapping

2015 ◽  
Vol 9 (1) ◽  
pp. 014101 ◽  
Author(s):  
D. Jin ◽  
B. Deng ◽  
J. X. Li ◽  
W. Cai ◽  
L. Tu ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
High Efficiency ◽  
Cell Trapping ◽  
Single Cell Trapping

A microfluidic device enabling deterministic single cell trapping and release

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Huichao Chai ◽  
Yongxiang Feng ◽  
Fei Liang ◽  
Wenhui Wang
Keyword(s):  
Chemical Analysis ◽  
Single Cell ◽  
Microfluidic Device ◽  
Single Cells ◽  
Cell Isolation ◽  
Cell Trapping ◽  
Analysis Techniques ◽  
Single Cell Trapping ◽  
Single Cell Isolation ◽  
Made In

Successful single-cell isolation is a pivotal technique for subsequent biological and chemical analysis of single cells. Although significant advances have been made in single-cell isolation and analysis techniques, most passive...

scholarly journals Parameter Screening in Microfluidics Based Hydrodynamic Single-Cell Trapping

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
B. Deng ◽  
X. F. Li ◽  
D. Y. Chen ◽  
L. D. You ◽  
J. B. Wang ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Single Cell Analysis ◽  
Fine Tuning ◽  
Trapping Efficiency ◽  
Cell Analysis ◽  
Cell Trapping ◽  
Micro Channels ◽  
Single Cell Trapping ◽  
Trapping Sites

Microfluidic cell-based arraying technology is widely used in the field of single-cell analysis. However, among developed devices, there is a compromise between cellular loading efficiencies and trapped cell densities, which deserves further analysis and optimization. To address this issue, the cell trapping efficiency of a microfluidic device with two parallel micro channels interconnected with cellular trapping sites was studied in this paper. By regulating channel inlet and outlet status, the microfluidic trapping structure can mimic key functioning units of previously reported devices. Numerical simulations were used to model this cellular trapping structure, quantifying the effects of channel on/off status and trapping structure geometries on the cellular trapping efficiency. Furthermore, the microfluidic device was fabricated based on conventional microfabrication and the cellular trapping efficiency was quantified in experiments. Experimental results showed that, besides geometry parameters, cellular travelling velocities and sizes also affected the single-cell trapping efficiency. By fine tuning parameters, more than 95% of trapping sites were taken by individual cells. This study may lay foundation in further studies of single-cell positioning in microfluidics and push forward the study of single-cell analysis.

scholarly journals Erratum: Integrated microfluidic device for single-cell trapping and spectroscopy

2013 ◽  
Vol 3 (1) ◽  
Author(s):  
C. Liberale ◽  
G. Cojoc ◽  
F. Bragheri ◽  
P. Minzioni ◽  
G. Perozziello ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Cell Trapping ◽  
Single Cell Trapping

Single-cell trapping and impedance measurement utilizing dielectrophoresis in a parallel-plate microfluidic device

2014 ◽  
Vol 190 ◽  
pp. 570-577 ◽  
Author(s):  
Nai-Chin Chen ◽  
Chun-Hong Chen ◽  
Ming-Kun Chen ◽  
Ling-Sheng Jang ◽  
Min-Haw Wang
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Parallel Plate ◽  
Impedance Measurement ◽  
Cell Trapping ◽  
Single Cell Trapping

scholarly journals Integrated microfluidic device for single-cell trapping and spectroscopy

2013 ◽  
Vol 3 (1) ◽  
Author(s):  
C. Liberale ◽  
G. Cojoc ◽  
F. Bragheri ◽  
P. Minzioni ◽  
G. Perozziello ◽  
...  
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Cell Trapping ◽  
Single Cell Trapping

An integrated microfluidic device for single cell trapping and osmotic behavior investigation of mouse oocytes

Cryobiology ◽  
2020 ◽  
Vol 92 ◽  
pp. 267-271 ◽  
Author(s):  
Xiaojie Guo ◽  
Zhongrong Chen ◽  
Kashan Memon ◽  
Xiaoyu Chen ◽  
Gang Zhao
Keyword(s):  
Single Cell ◽  
Microfluidic Device ◽  
Cell Trapping ◽  
Mouse Oocytes ◽  
Single Cell Trapping ◽  
Osmotic Behavior

scholarly journals Time Sequential Single-Cell Patterning with High Efficiency and High Density

Sensors ◽  
2018 ◽  
Vol 18 (11) ◽  
pp. 3672 ◽  
Author(s):  
Yang Liu ◽  
Dahai Ren ◽  
Xixin Ling ◽  
Weibin Liang ◽  
Jing Li ◽  
...  
Keyword(s):  
Single Cell ◽  
High Efficiency ◽  
Single Cells ◽  
Capture Efficiency ◽  
Jurkat Cells ◽  
Objective Lens ◽  
Cell Capture ◽  
Cell Trapping ◽  
Loop Channel ◽  
Single Cell Trapping

Single-cell capture plays an important role in single-cell manipulation and analysis. This paper presents a microfluidic device for deterministic single-cell trapping based on the hydrodynamic trapping mechanism. The device is composed of an S-shaped loop channel and thousands of aligned trap units. This arrayed structure enables each row of the device to be treated equally and independently, as it has row periodicity. A theoretical model was established and a simulation was conducted to optimize the key geometric parameters, and the performance was evaluated by conducting experiments on MCF-7 and Jurkat cells. The results showed improvements in single-cell trapping ability, including loading efficiency, capture speed, and the density of the patterned cells. The optimized device can achieve a capture efficiency of up to 100% and single-cell capture efficiency of up to 95%. This device offers 200 trap units in an area of 1 mm2, which enables 100 single cells to be observed simultaneously using a microscope with a 20× objective lens. One thousand cells can be trapped sequentially within 2 min; this is faster than the values obtained with previously reported devices. Furthermore, the cells can also be recovered by reversely infusing solutions. The structure can be easily extended to a large scale, and a patterned array with 32,000 trap sites was accomplished on a single chip. This device can be a powerful tool for high-throughput single-cell analysis, cell heterogeneity investigation, and drug screening.

Single cell trapping and stretching in a femtosecond laser fabricated optofluidic chip

2011 ◽  
Author(s):  
L. Ferrara ◽  
F. Bragheri ◽  
P. Minzioni ◽  
I. Cristiani ◽  
K. C. Vishnubhatla ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Single Cell ◽  
Cell Trapping ◽  
Single Cell Trapping ◽  
Optofluidic Chip
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