Label-free inertial-ferrohydrodynamic cell separation with high throughput and resolution

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yang Liu ◽  
Wujun Zhao ◽  
Rui Cheng ◽  
Alicia G Puig ◽  
Jamie Hodgson ◽  
...  
Keyword(s):  
High Throughput ◽  
Biological Samples ◽  
Cell Separation ◽  
Target Cells ◽  
Label Free ◽  
Advanced Technologies ◽  
Disease Diagnostics

Rapid and label-free separation of target cells in biological samples provided unique opportunity for disease diagnostics and treatment. However, even with advanced technologies for cell separation, the limiting throughput, high...

High-throughput rare cell separation from blood samples using steric hindrance and inertial microfluidics

Lab on a Chip ◽  
2014 ◽  
Vol 14 (14) ◽  
pp. 2525-2538 ◽  
Author(s):  
Shaofei Shen ◽  
Chao Ma ◽  
Lei Zhao ◽  
Yaolei Wang ◽  
Jian-Chun Wang ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
High Throughput ◽  
Steric Hindrance ◽  
Cell Separation ◽  
Label Free ◽  
Inertial Microfluidics ◽  
Blood Samples ◽  
Rare Cells

We present a multistage microfluidic device for continuous label-free separation of rare cells using a combination of inertial microfluidics and steric hindrance.

Automated Microfluidic Instrument for Label-Free and High-Throughput Cell Separation

2018 ◽  
Vol 90 (6) ◽  
pp. 4212-4220 ◽  
Author(s):  
Xinjie Zhang ◽  
Zhixian Zhu ◽  
Nan Xiang ◽  
Feifei Long ◽  
Zhonghua Ni
Keyword(s):  
High Throughput ◽  
Cell Separation ◽  
Label Free

scholarly journals Label-free ferrohydrodynamic cell separation of circulating tumor cells

Lab on a Chip ◽  
2017 ◽  
Vol 17 (18) ◽  
pp. 3097-3111 ◽  
Author(s):  
Wujun Zhao ◽  
Rui Cheng ◽  
Brittany D. Jenkins ◽  
Taotao Zhu ◽  
Nneoma E. Okonkwo ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Throughput ◽  
Recovery Rate ◽  
Cell Separation ◽  
Label Free ◽  
High Recovery ◽  
High Recovery Rate

A size-based ferrohydrodynamic cell separation (FCS) device capable of enriching intact circulating tumor cells with high throughput and high recovery rate.

scholarly journals Selection of aptamers against triple negative breast cancer cells using high throughput sequencing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Débora Ferreira ◽  
Joaquim Barbosa ◽  
Diana A. Sousa ◽  
Cátia Silva ◽  
Luís D. R. Melo ◽  
...  
Keyword(s):  
Breast Cancer ◽  
High Throughput ◽  
Triple Negative Breast Cancer ◽  
High Throughput Sequencing ◽  
Triple Negative ◽  
Metastatic Breast ◽  
Target Cells ◽  
Cancer Tissue ◽  
Surface Receptors

AbstractTriple-negative breast cancer is the most aggressive subtype of invasive breast cancer with a poor prognosis and no approved targeted therapy. Hence, the identification of new and specific ligands is essential to develop novel targeted therapies. In this study, we aimed to identify new aptamers that bind to highly metastatic breast cancer MDA-MB-231 cells using the cell-SELEX technology aided by high throughput sequencing. After 8 cycles of selection, the aptamer pool was sequenced and the 25 most frequent sequences were aligned for homology within their variable core region, plotted according to their free energy and the key nucleotides possibly involved in the target binding site were analyzed. Two aptamer candidates, Apt1 and Apt2, binding specifically to the target cells with $$K_{d}$$ K d values of 44.3 ± 13.3 nM and 17.7 ± 2.7 nM, respectively, were further validated. The binding analysis clearly showed their specificity to MDA-MB-231 cells and suggested the targeting of cell surface receptors. Additionally, Apt2 revealed no toxicity in vitro and showed potential translational application due to its affinity to breast cancer tissue sections. Overall, the results suggest that Apt2 is a promising candidate to be used in triple-negative breast cancer treatment and/or diagnosis.

scholarly journals Development of a Robust High-Throughput Screening Platform for Inhibitors of the Striatal-Enriched Tyrosine Phosphatase (STEP)

2021 ◽  
Vol 22 (9) ◽  
pp. 4417
Author(s):  
Lester J Lambert ◽  
Stefan Grotegut ◽  
Maria Celeridad ◽  
Palak Gosalia ◽  
Laurent JS De Backer ◽  
...  
Keyword(s):  
Drug Discovery ◽  
High Throughput ◽  
Tyrosine Kinases ◽  
High Throughput Screening ◽  
Kinase Inhibitors ◽  
Tyrosine Phosphatase ◽  
Protein Tyrosine Phosphatases ◽  
Synaptic Function ◽  
Label Free ◽  
Protein Tyrosine

Many human diseases are the result of abnormal expression or activation of protein tyrosine kinases (PTKs) and protein tyrosine phosphatases (PTPs). Not surprisingly, more than 30 tyrosine kinase inhibitors (TKIs) are currently in clinical use and provide unique treatment options for many patients. PTPs on the other hand have long been regarded as “undruggable” and only recently have gained increased attention in drug discovery. Striatal-enriched tyrosine phosphatase (STEP) is a neuron-specific PTP that is overactive in Alzheimer’s disease (AD) and other neurodegenerative and neuropsychiatric disorders, including Parkinson’s disease, schizophrenia, and fragile X syndrome. An emergent model suggests that the increase in STEP activity interferes with synaptic function and contributes to the characteristic cognitive and behavioral deficits present in these diseases. Prior efforts to generate STEP inhibitors with properties that warrant clinical development have largely failed. To identify novel STEP inhibitor scaffolds, we developed a biophysical, label-free high-throughput screening (HTS) platform based on the protein thermal shift (PTS) technology. In contrast to conventional HTS using STEP enzymatic assays, we found the PTS platform highly robust and capable of identifying true hits with confirmed STEP inhibitory activity and selectivity. This new platform promises to greatly advance STEP drug discovery and should be applicable to other PTP targets.

scholarly journals Development of a Novel Label-Free and High-Throughput Arginase-1 Assay Using Self-Assembled Monolayer Desorption Ionization Mass Spectrometry

2021 ◽  
pp. 247255522110006
Author(s):  
Michael D. Scholle ◽  
Zachary A. Gurard-Levin
Keyword(s):  
Mass Spectrometry ◽  
Drug Discovery ◽  
High Throughput ◽  
Ionization Mass Spectrometry ◽  
Ionization Mass ◽  
Label Free ◽  
Desorption Ionization ◽  
Arginase 1 ◽  
Self Assembled ◽  
High Throughput Assay

Arginase-1, an enzyme that catalyzes the reaction of L-arginine to L-ornithine, is implicated in the tumor immune response and represents an interesting therapeutic target in immuno-oncology. Initiating arginase drug discovery efforts remains a challenge due to a lack of suitable high-throughput assay methodologies. This report describes the combination of self-assembled monolayers and matrix-assisted laser desorption ionization mass spectrometry to enable the first label-free and high-throughput assay for arginase activity. The assay was optimized for kinetically balanced conditions and miniaturized, while achieving a robust assay (Z-factor > 0.8) and a significant assay window [signal-to-background ratio > 20] relative to fluorescent approaches. To validate the assay, the inhibition of the reference compound nor-NOHA (Nω-hydroxy-nor-L-arginine) was evaluated, and the IC50 measured to be in line with reported results (IC50 = 180 nM). The assay was then used to complete a screen of 175,000 compounds, demonstrating the high-throughput capacity of the approach. The label-free format also eliminates opportunities for false-positive results due to interference from library compounds and optical readouts. The assay methodology described here enables new opportunities for drug discovery for arginase and, due to the assay flexibility, can be more broadly applicable for measuring other amino acid–metabolizing enzymes.

Clog-free cell filtration using resettable cell traps

Lab on a Chip ◽  
2014 ◽  
Vol 14 (15) ◽  
pp. 2657-2665 ◽  
Author(s):  
William Beattie ◽  
Xi Qin ◽  
Lin Wang ◽  
Hongshen Ma
Keyword(s):  
High Throughput ◽  
Cell Separation ◽  
Free Cell ◽  
Separation Mechanism ◽  
Cell Filtration ◽  
Capture And Release

A microfluidic cell separation mechanism created using constrictions with adjustable size that can selectively capture and release cells, thereby enabling high throughput size and deformability based cell separation without clogging.

Label free high throughput screening for apoptosis inducing chemicals using time-lapse microscopy signal processing

APOPTOSIS ◽  
2014 ◽  
Vol 19 (9) ◽  
pp. 1411-1418 ◽  
Author(s):  
Obaid Aftab ◽  
Madiha Nazir ◽  
Mårten Fryknäs ◽  
Ulf Hammerling ◽  
Rolf Larsson ◽  
...  
Keyword(s):  
Signal Processing ◽  
High Throughput ◽  
High Throughput Screening ◽  
Time Lapse ◽  
Label Free ◽  
Time Lapse Microscopy
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