scholarly journals Label-free ferrohydrodynamic cell separation of circulating tumor cells

Lab on a Chip ◽  
2017 ◽  
Vol 17 (18) ◽  
pp. 3097-3111 ◽  
Author(s):  
Wujun Zhao ◽  
Rui Cheng ◽  
Brittany D. Jenkins ◽  
Taotao Zhu ◽  
Nneoma E. Okonkwo ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Throughput ◽  
Recovery Rate ◽  
Cell Separation ◽  
Label Free ◽  
High Recovery ◽  
High Recovery Rate

A size-based ferrohydrodynamic cell separation (FCS) device capable of enriching intact circulating tumor cells with high throughput and high recovery rate.

Fundamentals of integrated ferrohydrodynamic cell separation in circulating tumor cell isolation

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Yang Liu ◽  
Wujun Zhao ◽  
Rui Cheng ◽  
Bryana N. Harris ◽  
Jonathan R. Murrow ◽  
...  
Keyword(s):  
Tumor Cell ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Recovery Rate ◽  
Cell Separation ◽  
Circulating Tumor Cell ◽  
Fundamental Theory ◽  
High Recovery ◽  
High Recovery Rate ◽  
Experimental Validations

We present the fundamental theory and experimental validations of an integrated ferrohydrodynamic cell separation (iFCS) method that can isolate circulating tumor cells with a high recovery rate.

scholarly journals High-Throughput Microfluidic Labyrinth for the Label-free Isolation of Circulating Tumor Cells

Cell Systems ◽  
2017 ◽  
Vol 5 (3) ◽  
pp. 295-304.e4 ◽  
Author(s):  
Eric Lin ◽  
Lianette Rivera-Báez ◽  
Shamileh Fouladdel ◽  
Hyeun Joong Yoon ◽  
Stephanie Guthrie ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Throughput ◽  
Label Free

On the Design of a DLD-DEP Device for Separation of Circulating Tumor Cells in Blood

2020 ◽  
Author(s):  
Mehdi Rahmati ◽  
Xiaolin Chen
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Cancer Progression ◽  
High Throughput ◽  
Blood Cells ◽  
Lateral Displacement ◽  
Uniform Electric Field ◽  
Label Free ◽  
Particle Tracing ◽  
Key Factor

Abstract Circulating Tumor Cells (CTCs), which migrate from original sites in a body to distant organs through blood, are a key factor in cancer detection. Emerging Label-free techniques owing to their inherent advantage to preserve characteristics of sorted cells and low consumption of samples can be promising to the prediction of cancer progression and metastasis research. Deterministic Lateral Displacement (DLD) is one of the label-free separation techniques employing a specific arrangement of micro-posts for continuous separation of suspended cells in a buffer based on the size of cells. Separation based solely on size is challenging since the size distributions of CTCs might overlap with those of normal blood cells. To address this problem, DLD can be combined with dielectrophoresis (DEP) technique which is the phenomenon of particle movement in a non-uniform electric field owing to the polarization effect. Although, DLD devices employ the laminar flow in low Reynolds number (Re) fluid flow due to predictability of such flow regimes, they should be improved to work in higher Re flow regime so as to attain high throughput devices. In this paper, a particle tracing simulation is developed to study the effects of different post shapes, shift fraction of micropost arrays, and dielectrophoresis forces on separation of CTCs from peripheral blood cells. Our numerical model and results provide a groundwork for design and fabrication of high-throughput DLD-DEP devices for improvement of CTC separation.

scholarly journals Label-free ferrohydrodynamic separation of exosome-like nanoparticles

Lab on a Chip ◽  
2020 ◽  
Vol 20 (17) ◽  
pp. 3187-3201
Author(s):  
Yang Liu ◽  
Wujun Zhao ◽  
Rui Cheng ◽  
Meghan Logun ◽  
Maria del Mar Zayas-Viera ◽  
...  
Keyword(s):  
Recovery Rate ◽  
Label Free ◽  
High Recovery ◽  
Size Dependent ◽  
High Recovery Rate

Particle ferrohydrodynamics and its device (FerroChip) enables label-free and size-dependent separation of exosome-like nanoparticles with high recovery rate and purity.

Enhancing the Cell Viability in High Throughput Deterministic Lateral Displacement Separation of Circulating Tumor Cells

2019 ◽  
Author(s):  
Arian Aghilinejad ◽  
Christopher Landry ◽  
George Cha ◽  
Xiaolin Chen
Keyword(s):  
Reynolds Number ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Throughput ◽  
Cancer Metastasis ◽  
Lateral Displacement ◽  
Shear Stresses ◽  
Label Free ◽  
Deterministic Lateral Displacement ◽  
Reynolds Number Flow

Abstract Cancer is among a major health concerns all over the world. Cancer metastasis, which defines as the migration of malignant cells from original sites to distant organs, is the main reason of death due to cancer and there is growing evidence that Circulating Tumor Cells (CTCs) are responsible for initiating the metastasis. Due to the importance of these bioparticles in biotechnology and medicine, there is a growing interest to study and separate them through different techniques especially microfluidic label-free technologies. One such technology, termed Deterministic Lateral Displacement (DLD) has recently shown promising abilities to separate cells and particles of different sizes. However, DLD is a separation method that takes advantages of the predictable flow laminae of low Reynolds number (Re) fluid flow. In order to achieve higher device throughput, effects of higher Reynolds number flow on DLD device should be studied. Additionally, the higher flow rates would apply higher forces and shear stresses on the cells which threaten the cell’s viability. In this study, employing numerical simulation, the effect of high Re number on DLD device for separating cancer cells has been investigated. Specifically, we conducted force analysis and by focusing on the downstream gap distance between the posts, we improved the device which results in less cell deformation. Our developed numerical model and presented results lay the groundwork for design and fabrication of high-throughput DLD microchips for enhanced separation of CTCs.

Rapid liquid biopsy for Mohs surgery: rare target cell separation from surgical margin lavage fluid with a high recovery rate and selectivity

Lab on a Chip ◽  
2019 ◽  
Vol 19 (6) ◽  
pp. 974-983
Author(s):  
Wenbo Zhou ◽  
Yaoping Liu ◽  
Menglong Ran ◽  
Xiaofan Zhao ◽  
Hang Li ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Target Cell ◽  
Liquid Biopsy ◽  
Cell Separation ◽  
Surgical Margin ◽  
Residual Tumor ◽  
Lavage Fluid ◽  
High Recovery ◽  
Mohs Surgery ◽  
High Recovery Rate

A liquid biopsy was established for rapid and sensitive examination of residual tumor cells on surgical margin during Mohs surgery.

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