Protective effect of Bifidobacterium bifidium FSDJN7O5 and Bifidobacterium breve FHNFQ23M3 on diarrhea caused by enterotoxigenic Escherichia coli

2021 ◽  
Author(s):  
Bo Yang ◽  
Zheng Huang ◽  
Zhujun He ◽  
Yue Yue ◽  
Yonghua Zhou ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Developing Countries ◽  
Protective Effect ◽  
Enterotoxigenic Escherichia Coli ◽  
Mice Model ◽  
Bifidobacterium Breve ◽  
Traveler’S Diarrhea ◽  
Traveler's Diarrhea

Enterotoxigenic Escherichia coli (ETEC) is the main bacterial cause for diarrhea among children in the developing countries and traveler’s diarrhea. In this study, mice model was used to evaluate the...

scholarly journals Complete Genome Sequence of Enterotoxigenic Escherichia coli Siphophage LL5

2019 ◽  
Vol 8 (27) ◽  
Author(s):  
Denish Piya ◽  
Lauren Lessor ◽  
Mei Liu ◽  
Jason J. Gill
Keyword(s):  
Escherichia Coli ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Enterotoxigenic Escherichia Coli ◽  
Content Type ◽  
Traveler’S Diarrhea ◽  
Traveler's Diarrhea

ABSTRACT Here, we describe the complete genome sequence of siphophage LL5. LL5 is a T1-like phage isolated against enterotoxigenic Escherichia coli, which causes traveler’s diarrhea. LL5 is included as a component phage in the commercial prebiotic product PreforPro.

scholarly journals Laboratory investigation of diarrhea in travelers to Mexico: evaluation of methods for detecting enterotoxigenic Echerichia coli

1976 ◽  
Vol 3 (5) ◽  
pp. 486-495
Author(s):  
G K Morris ◽  
M H Merson ◽  
D A Sack ◽  
J G Wells ◽  
W T Martin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Laboratory Investigation ◽  
Chinese Hamster ◽  
Enterotoxigenic Escherichia Coli ◽  
Ileal Loop ◽  
Adrenal Cell ◽  
Infant Mouse ◽  
Traveler’S Diarrhea ◽  
Cell Assay ◽  
Traveler's Diarrhea

A laboratory investigation was conducted on cultures collected from travelers before, during, and after a trip to Mexico to characterize the etiology of traveler's diarrhea. Four laboratory methods for detecting enterotoxigenicity of Escherichia coli were evaluated: the infant mouse assay, the Chinese hamster ovary (CHO) cell assay, the Y1 adrenal cell assay, and the rabbit ileal loop. Although a number of common enteric pathogens were identified as a cause of traveler's diarrhea, including six serotypes of Salmonella, two serotypes of Shigella, Vibrio parahaemolyticus, Giardia lamblia, and Entamoeba histolytica, enterotoxigenic Escherichia coli was most commonly isolated. Strains were identified that produced only heat-labile enterotoxin (LT), only heat-stable enterotoxin (ST), or both LT and ST. The infant mouse assay yielded results falling into two distinct groups, providing a clear separation of positive and negative cultures. The CHO assay also formed two groups, with positive cultures producing 11% or more of the elongated cells. There was good agreement between the CHO and the Y1 adrenal cell assays for detection of LT. The adrenal cell system for detection of LT was more suitable than the CHO assay for processing large numbers of specimens because of the miniculture modification of this method utilized in this study. The infant mouse method was a simple and reliable method for detecting ST.

scholarly journals Complete Genome Sequence of Enterotoxigenic Escherichia coli Myophage LL12

2019 ◽  
Vol 8 (30) ◽  
Author(s):  
Denish Piya ◽  
Lauren Lessor ◽  
Mei Liu ◽  
Jason J. Gill
Keyword(s):  
Escherichia Coli ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Enterotoxigenic Escherichia Coli ◽  
Content Type ◽  
Traveler’S Diarrhea ◽  
Traveler's Diarrhea

This work describes the complete genome sequence of the virulent myophage LL12. The 136-kb LL12 genome is related to coliphage V5 and is a component in the prebiotic PreforPro. LL12 was isolated against enterotoxigenic Escherichia coli, which causes traveler’s diarrhea.

scholarly journals Genotypic Characterization of Enterotoxigenic Escherichia coli Strains Causing Traveler's Diarrhea

2012 ◽  
Vol 51 (2) ◽  
pp. 633-635 ◽  
Author(s):  
F. P. Rivera ◽  
A. M. Medina ◽  
E. Aldasoro ◽  
A. Sangil ◽  
J. Gascon ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Enterotoxigenic Escherichia Coli ◽  
Traveler’S Diarrhea ◽  
Genotypic Characterization ◽  
Traveler's Diarrhea

scholarly journals Emergence of Resistance to Quinolones and β-Lactam Antibiotics in Enteroaggregative and Enterotoxigenic Escherichia coli Causing Traveler’s Diarrhea

2018 ◽  
Vol 63 (2) ◽  
pp. e01745-18 ◽  
Author(s):  
Elisabet Guiral ◽  
Milene Gonçalves Quiles ◽  
Laura Muñoz ◽  
Javier Moreno-Morales ◽  
Izaskun Alejo-Cancho ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Good Alternative ◽  
Sub Saharan Africa ◽  
Enterotoxigenic Escherichia Coli ◽  
Content Type ◽  
Traveler’S Diarrhea ◽  
Antimicrobial Resistance Genes ◽  
Sub Saharan ◽  
Traveler's Diarrhea

ABSTRACT The objective of this study was to assess the antimicrobial resistance of enteroaggregative Escherichia coli (EAEC) and enterotoxigenic E. coli (ETEC) strains causing traveler’s diarrhea (TD) and to investigate the molecular characterization of antimicrobial resistance genes to third-generation cephalosporins, cephamycins, and quinolones. Overall, 39 EAEC and 43 ETEC clinical isolates were studied. The susceptibilities of EAEC and ETEC against ampicillin, amoxicillin-clavulanic acid, cefotaxime, imipenem, chloramphenicol, tetracycline, co-trimoxazole, nalidixic acid, ciprofloxacin, azithromycin, and rifaximin were determined. All genes encoding resistance determinants were detected by PCR or PCR plus DNA sequencing. The epidemiology of selected EAEC and ETEC strains was studied using multilocus sequence typing (MLST). The resistance to quinolones of EAEC and ETEC strains causing TD has significantly increased over the last decades, and high percentages have been found especially in patients traveling to India and sub-Saharan Africa. Sequence type 38 (ST38) and ST131, carrying the blaCTX-M-15 and blaCTX-M-27 genes, respectively, are highly prevalent among extended-spectrum β-lactamase (ESBL)-producing EAEC and ETEC strains. The cephamycinase ACT-20 is described in the present study for the first time in EAEC and ETEC strains causing TD in patients who had traveled to Central America. The percentages of resistance to azithromycin in EAEC and ETEC isolates from patients to Southeast Asia/India and Africa are above 25%. Meanwhile, rifaximin is still active against EAEC and ETEC, with the prevalence of resistant strains not being high. In conclusion, fluoroquinolones should no longer be considered the drugs of choice for the prevention or treatment in TD for travelers traveling to India and Africa. Azithromycin and rifaximin are still a good alternative to treat TD caused by EAEC or ETEC.

Detection of Enterotoxigenic Escherichia coli in Rotavirus-Infected Ghanaian Children Diagnosed with Acute Gastroenteritis

2021 ◽  
Author(s):  
Bartholomew Dzudzor ◽  
Albert Amenyedor ◽  
Vincent Amarh ◽  
George E. Armah
Keyword(s):  
Escherichia Coli ◽  
Developing Countries ◽  
Enterotoxigenic Escherichia Coli ◽  
Surveillance Study ◽  
Vaccination Program ◽  
E Coli ◽  
Rotavirus Vaccination ◽  
Stool Samples ◽  
Global Health Problem ◽  
Vaccine Surveillance

Diarrhea is a notable global health problem in several developing countries, especially in children. Prior to the introduction of the rotavirus vaccination program in Ghana, a surveillance study was conducted to investigate the prevalence of the disease caused by rotavirus in children. In this report, we re-used archival stool samples from the pre-vaccine surveillance study to provide information on prevalence of enterotoxigenic Escherichia coli in Ghanaian children. Re-analysis of the stool samples revealed co-infection of enterotoxigenic E. coli and rotavirus in 2% of the children whose samples were selected for this study. As Ghana is approaching 10 years post-implementation of the rotavirus vaccination program, the preliminary data presented in this report are a vital reference for subsequent studies aimed at ascertaining the effect of the vaccine on both rotavirus and enterotoxigenic E. coli.

scholarly journals Enteroaggregative Escherichia coli as a Cause of Traveler's Diarrhea: Clinical Response to Ciprofloxacin

1999 ◽  
Vol 29 (2) ◽  
pp. 335-338 ◽  
Author(s):  
Mariela Glandt ◽  
Javier A. Adachi ◽  
John J. Mathewson ◽  
Zhi‐Dong Jiang ◽  
Daniel DiCesare ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Clinical Response ◽  
Traveler’S Diarrhea ◽  
Traveler's Diarrhea
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