Bioinspired dopamine and zwitterionic polymers for non-fouling surface engineering

2021 ◽  
Author(s):  
Anika Benozir Asha ◽  
Yangjun Chen ◽  
Ravin Narain
Keyword(s):  
Medical Devices ◽  
Surface Engineering ◽  
Zwitterionic Polymers ◽  
Zwitterionic Polymer ◽  
Non Fouling

Conjugation of zwitterionic polymer with dopamine is a promising approach to endow the surface with antifouling properties for medical devices, biosensors and marine coating applications etc. Zwitterionic polymers can be conjugated with dopamine by mostly four methods.

scholarly journals Bioactive Coatings with Ag-Camphorimine Complexes to Prevent Surface Colonization by the Pathogenic Yeast Candida albicans

Antibiotics ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 638
Author(s):  
M. Joana F. Pinheiro ◽  
Joana P. Costa ◽  
Fernanda Marques ◽  
Nuno P. Mira ◽  
M. Fernanda N. N. Carvalho ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Medical Devices ◽  
Mammalian Cells ◽  
Surface Engineering ◽  
Pathogenic Yeast ◽  
Bioactive Coatings ◽  
Clinical Strains ◽  
Persistent Infections ◽  
Compound Q ◽  
Significant Step

Currently there is a gap between the rate of new antifungal development and the emergence of resistance among Candida clinical strains, particularly threatened by the extreme adhesiveness of C. albicans to indwelling medical devices. Two silver camphorimine complexes, [Ag(OH){OC10H14N(C6H4)2NC10H14O}] (compound P) and [{Ag(OC10H14NC6H4CH3-p)}2(μ-O)] (compound Q), are herein demonstrated as having high inhibiting activity towards the growth of Candida albicans and Candida glabrata clinical strains resistant to azoles, the frontline antifungals used in clinical practice. Compounds P and Q were also explored as bioactive coatings to prevent colonization by C. albicans and colonize the surface of indwelling medical devices, resulting in persistent infections. Functionalization of stainless steel with polycaprolactone (PCL) matrix embedded with compounds P or Q was reported for the first time to inhibit the colonization of C. albicans by 82% and 75%, respectively. The coating of PCL loaded with Q or P did not cause cytotoxic effects in mammalian cells, demonstrating the biocompatibility of the explored approach. The identification and further exploration of new approaches for surface engineering based on new molecules that can sensitize resistant strains, as herein demonstrated for complexes P and Q, is a significant step forward to improve the successful treatment of candidiasis.

scholarly journals P4VP Modified Zwitterionic Polymer for the Preparation of Antifouling Functionalized Surfaces

Nanomaterials ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 706 ◽  
Author(s):  
Chaoqun Wu ◽  
Yudan Zhou ◽  
Haitao Wang ◽  
Jianhua Hu
Keyword(s):  
Photoelectron Spectroscopy ◽  
Cell Attachment ◽  
Raft Polymerization ◽  
Polymer Brush ◽  
Contact Angles ◽  
Polymerization Process ◽  
Water Contact ◽  
Zwitterionic Polymers ◽  
Ito Glass ◽  
Zwitterionic Polymer

Zwitterionic polymers are suitable for replacing poly(ethylene glycol) (PEG) polymers because of their better antifouling properties, but zwitterionic polymers have poor mechanical properties, strong water absorption, and their homopolymers should not be used directly. To solve these problems, a reversible-addition fragmentation chain transfer (RAFT) polymerization process was used to prepare copolymers comprised of zwitterionic side chains that were attached to an ITO glass substrate using spin-casting. The presence of 4-vinylpyridine (4VP) and zwitterion chains on these polymer-coated ITO surfaces was confirmed using 1H NMR, FTIR, and GPC analyses, with successful surface functionalization confirmed using water contact angle, X-ray photoelectron spectroscopy (XPS), and atomic force microscopy (AFM) studies. Changes in water contact angles and C/O ratios (XPS) analysis demonstrated that the functionalization of these polymers with β-propiolactone resulted in hydrophilic mixed 4VP/zwitterionic polymers. Protein adsorption and cell attachment assays were used to optimize the ratio of the zwitterionic component to maximize the antifouling properties of the polymer brush surface. This work demonstrated that the antifouling surface coatings could be readily prepared using a “P4VP-modified” method, that is, the functionality of P4VP to modify the prepared zwitterionic polymer. We believe these materials are likely to be useful for the preparation of biomaterials for biosensing and diagnostic applications.

scholarly journals Fouling-resistant zwitterionic polymers for complete prevention of postoperative adhesion

2020 ◽  
Vol 117 (50) ◽  
pp. 32046-32055
Author(s):  
Ershuai Zhang ◽  
Boyi Song ◽  
Yuanjie Shi ◽  
Hui Zhu ◽  
Xiangfei Han ◽  
...  
Keyword(s):  
Protective Layer ◽  
Pelvic Surgery ◽  
Postoperative Adhesion ◽  
Postoperative Adhesions ◽  
Working Mechanism ◽  
Zwitterionic Polymers ◽  
Physical Barriers ◽  
Zwitterionic Polymer ◽  
Protein Cell ◽  
Prevention Approach

Postoperative adhesions are most common issues for almost any types of abdominal and pelvic surgery, leading to adverse consequences. Pharmacological treatments and physical barrier devices are two main approaches to address postoperative adhesions but can only alleviate or reduce adhesions to some extent. There is an urgent need for a reliable approach to completely prevent postoperative adhesions and to significantly improve the clinical outcomes, which, however, is unmet with current technologies. Here we report that by applying a viscous, cream-like yet injectable zwitterionic polymer solution to the traumatized surface, postoperative adhesion was completely and reliably prevented in three clinically relevant but increasingly challenging models in rats. The success rate of full prevention is over 93% among 42 animals tested, which is a major leap in antiadhesion performance. Clinically used Interceed film can hardly prevent the adhesion in any of these models. Unlike current antiadhesion materials serving solely as physical barriers, the “nonfouling” zwitterionic polymer functioned as a protective layer for antiadhesion applications with the inherent benefit of resisting protein/cell adhesions. The nonfouling nature of the polymer prevented the absorption of fibronectins and fibroblasts, which contribute to the initial and late-stage development of the adhesion, respectively. This is the key working mechanism that differentiated our “complete prevention” approach from current underperforming antiadhesion materials. This work implies a safe, effective, and convenient way to fully prevent postoperative adhesions suffered by current surgical patients.

An introduction to zwitterionic polymer behavior and applications in solution and at surfaces

2019 ◽  
Vol 48 (3) ◽  
pp. 757-770 ◽  
Author(s):  
Lewis D. Blackman ◽  
Pathiraja A. Gunatillake ◽  
Peter Cass ◽  
Katherine E. S. Locock
Keyword(s):  
Zwitterionic Polymers ◽  
Zwitterionic Polymer

Zwitterionic polymers, including polyampholytes and polybetaines, are polymers with both positive and negative charges incorporated into their structure.

scholarly journals De novo design of functional zwitterionic biomimetic material for immunomodulation

2020 ◽  
Vol 6 (22) ◽  
pp. eaba0754 ◽  
Author(s):  
Bowen Li ◽  
Zhefan Yuan ◽  
Priyesh Jain ◽  
Hsiang-Chieh Hung ◽  
Yuwei He ◽  
...  
Keyword(s):  
De Novo ◽  
Specific Interaction ◽  
De Novo Design ◽  
The Other ◽  
Trade Off ◽  
Zwitterionic Polymers ◽  
Zwitterionic Polymer ◽  
Nonspecific Interactions ◽  
Immunomodulatory Properties

Superhydrophilic zwitterionic polymers are a class of nonfouling materials capable of effectively resisting any nonspecific interactions with biological systems. We designed here a functional zwitterionic polymer that achieves a trade-off between nonspecific interactions providing the nonfouling property and a specific interaction for bioactive functionality. Built from phosphoserine, an immune-signaling molecule in nature, this zwitterionic polymer exhibits both nonfouling and immunomodulatory properties. Its conjugation to uricase is shown to proactively eradicate all unwanted immune response, outperforming the zwitterionic polymers. On the other hand, this polymer could significantly prolong the half-life of protein drugs in vivo, overcoming the innate drawback of phosphoserine in inducing accelerated clearance. Our demonstration of a nonfouling zwitterionic material with built-in immunomodulatory functionality provides new insights into the fundamental design of biomaterials, as well as far-reaching implications for broad applications such as drug delivery, implants, and cell therapy.

scholarly journals Application of Nanoscale Zwitterionic Polyelectrolytes Brush with High Stability and Quantum Yield in Aqueous Solution for Cell Imaging

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Zhiyong Zhang ◽  
Minqiang Tan ◽  
Linghui Kong ◽  
Xiaomei Lu ◽  
Pengfei Sun ◽  
...  
Keyword(s):  
Quantum Yield ◽  
Water Solubility ◽  
Cationic Polymer ◽  
Side Chains ◽  
Zwitterionic Polymers ◽  
Zwitterionic Polymer ◽  
Low Cytotoxicity ◽  
Ph Range ◽  
Cationic Polyelectrolytes

Cationic and zwitterionic polyelectrolytes are synthesized through atom transfer radical polymerization (ATRP), comprising a polyfluorene backbone with a small fraction of 2,1,3-benzothiadiazole and poly[2-(dimethylamino)ethyl methacrylate] (PDMAEMA) side chains. Due to higher charge density generated from grafted side chains, two polymers show higher water solubility and higher quantum yield. In comparison with cationic polyelectrolytes, zwitterionic polyelectrolytes are stable over a broad pH range from 1 to 13, even in 1 M NaCl solution. The absence of FRET between zwitterionic polymers and dye-labeled ssDNA indicates their ultralow nonspecific adsorption, while cationic polymer shows much stronger nonspecific interactions. The MTT assay of zwitterionic polymers exhibits their minimal cytotoxicity and potential in long-term clinical application. Most importantly, zwitterionic polymer could be efficiently taken up by cells, whereas cationic polymer stains the surface of cell due to membrane disruption generated from positive charges. The results illustrate that conjugated zwitterionic polymer could serve as a novel type of highly efficient ultralow fouling material with low cytotoxicity for labelling cell or potential biomedical applications.

Water Structure Change-Induced Expansion and Collapse of Zwitterionic Polymers Surface-Grafted onto Carbon Black

2014 ◽  
Vol 67 (11) ◽  
pp. 1706
Author(s):  
Yiwen Pei ◽  
Jadranka Travas-Sejdic ◽  
David E. Williams
Keyword(s):  
Carbon Black ◽  
Protein Stability ◽  
Polymer Brushes ◽  
Water Structure ◽  
Dipole Interaction ◽  
Structure Change ◽  
Zwitterionic Polymers ◽  
Zwitterionic Polymer

We demonstrate the expansion and collapse of surface-grafted zwitterionic polymer brushes in water caused by the addition of urea. We hypothesize that at low urea concentrations, this is an effect of an ion–dipole interaction between urea and the polymer, and at high urea concentrations, an effect of a change in water structure causing change in solvation of the brushes and hence a change in the dipole–dipole interaction, and that it is analogous to the effects of urea on protein stability.

Ultrastructural changes in murine lymphoma cells exposed to ultraviolet-A radiation

1991 ◽  
Vol 49 ◽  
pp. 104-105
Author(s):  
Delma P. Thomas ◽  
Dianne E. Godar
Keyword(s):  
Medical Devices ◽  
Blood Cells ◽  
White Blood Cells ◽  
Consumer Products ◽  
Ultrastructural Changes ◽  
Ultraviolet A ◽  
Uv Spectrum ◽  
Lymphoma Cells ◽  
Murine Lymphoma ◽  
Transmission Electron

Ultraviolet radiation (UVR) from all three waveband regions of the UV spectrum, UVA (320-400 nm), UVB (290-320 nm), and UVC (200-290 nm), can be emitted by some medical devices and consumer products. Sunlamps can expose the blood to a considerable amount of UVR, particularly UVA and/or UVB. The percent transmission of each waveband through the epidermis to the dermis, which contains blood, increases in the order of increasing wavelength: UVC (10%) < UVB (20%) < UVA (30%). To investigate the effects of UVR on white blood cells, we chose transmission electron microscopy to examine the ultrastructure changes in L5178Y-R murine lymphoma cells.

Bacterial biofilm behavior in water-supply lines of dental units

1992 ◽  
Vol 50 (1) ◽  
pp. 882-883
Author(s):  
B.D. Tall ◽  
K.S. George ◽  
R. T. Gray ◽  
H.N. Williams
Keyword(s):  
Water Supply ◽  
Medical Devices ◽  
Biofilm Formation ◽  
Bacterial Biofilm

Studies of bacterial behavior in many environments have shown that most organisms attach to surfaces, forming communities of microcolonies called biofilms. In contaminated medical devices, biofilms may serve both as reservoirs and as inocula for the initiation of infections. Recently, there has been much concern about the potential of dental units to transmit infections. Because the mechanisms of biofilm formation are ill-defined, we investigated the behavior and formation of a biofilm associated with tubing leading to the water syringe of a dental unit over a period of 1 month.

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