Shedding light on ultrafast ring-twisting pathways of halogenated GFP chromophores from the excited to ground state

2021 ◽  
Author(s):  
Sean Boulanger ◽  
Cheng Chen ◽  
Longteng Tang ◽  
Liangdong Zhu ◽  
Nadezhda S. Baleeva ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Ground State ◽  
Fluorescent Protein ◽  
Fluorescence Properties ◽  
Cellular Biology ◽  
Keen Interest ◽  
Green Fluorescent

Since green fluorescent protein (GFP) has revolutionized molecular and cellular biology for about three decades, there has been a keen interest in understanding, designing, and controlling the fluorescence properties of...

Ultrafast Excited and Ground-State Dynamics of the Green Fluorescent Protein Chromophore in Solution

2004 ◽  
Vol 108 (21) ◽  
pp. 4587-4598 ◽  
Author(s):  
Mikas Vengris ◽  
Ivo H. M. van Stokkum ◽  
Xiang He ◽  
Alasdair F. Bell ◽  
Peter J. Tonge ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Ground State ◽  
Fluorescent Protein ◽  
Green Fluorescent

Electric field effect on the ground state proton transfer in the H-bonded HBDI complex: an implication of the green fluorescent protein

RSC Advances ◽  
2014 ◽  
Vol 4 (51) ◽  
pp. 26543-26551 ◽  
Author(s):  
Baotao Kang ◽  
Hongguang Liu ◽  
Du-Jeon Jang ◽  
Jin Yong Lee
Keyword(s):  
Green Fluorescent Protein ◽  
Electric Field ◽  
Proton Transfer ◽  
Ground State ◽  
First Principles ◽  
Field Effect ◽  
Fluorescent Protein ◽  
Electric Field Effect ◽  
First Principles Calculations ◽  
Green Fluorescent

In this paper, first-principles calculations were performed regarding the electric field effect on the ground state proton transfer (GSPT) in the H-bonded p-hydroxybenzylideneimidazolidinone (HBDI) network that represents the active site of the green fluorescent protein (GFP).

Synthesis and fluorescence properties of 4-diarylmethylene analogues of the green fluorescent protein chromophore

Tetrahedron ◽  
2015 ◽  
Vol 71 (30) ◽  
pp. 4987-4998 ◽  
Author(s):  
Masahiro Ikejiri ◽  
Kousuke Matsumoto ◽  
Hiraku Hasegawa ◽  
Daisuke Yamaguchi ◽  
Moe Tsuchino ◽  
...  
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Fluorescence Properties ◽  
Green Fluorescent

scholarly journals Influence of nanobody binding on fluorescence emission, mobility and organization of GFP-tagged proteins

2020 ◽  
Author(s):  
Falk Schneider ◽  
Christian Eggeling ◽  
Erdinc Sezgin
Keyword(s):  
Green Fluorescent Protein ◽  
Fluorescence Microscopy ◽  
Fluorescent Protein ◽  
Fluorescent Proteins ◽  
Fluorescence Emission ◽  
Fluorescence Properties ◽  
Great Care ◽  
Target Molecules ◽  
Green Fluorescent

SummaryAdvanced fluorescence microscopy studies require specific and monovalent molecular labelling with bright and photostable fluorophores. This necessity led to the widespread use of fluorescently labelled nanobodies against commonly employed fluorescent proteins. However, very little is known how these nanobodies influence their target molecules. Here, we observed clear changes of the fluorescence properties, mobility and organisation of green fluorescent protein (GFP) tagged proteins after labelling with an anti-GFP nanobody. Intriguingly, we did not observe any co-diffusion of fluorescently-labelled nanobodies with the GFP-labelled proteins. Our results suggest significant binding of the nanobodies to a non-emissive, oligomerized form of the fluorescent proteins, promoting disassembly into more monomeric forms after binding. Our findings show that great care must be taken when using nanobodies for studying dynamic and quantitative protein organisation.

scholarly journals Ground-State Proton Transfer Kinetics in Green Fluorescent Protein

Biochemistry ◽  
2014 ◽  
Vol 53 (37) ◽  
pp. 5947-5957 ◽  
Author(s):  
Luke M. Oltrogge ◽  
Quan Wang ◽  
Steven G. Boxer
Keyword(s):  
Green Fluorescent Protein ◽  
Proton Transfer ◽  
Ground State ◽  
Fluorescent Protein ◽  
Green Fluorescent

Effects of Threonine 203 Replacements on Excited-State Dynamics and Fluorescence Properties of the Green Fluorescent Protein (GFP)

2000 ◽  
Vol 104 (19) ◽  
pp. 4791-4798 ◽  
Author(s):  
Andreas D. Kummer ◽  
Jens Wiehler ◽  
Hermann Rehaber ◽  
Christian Kompa ◽  
Boris Steipe ◽  
...  
Keyword(s):  
Excited State ◽  
Green Fluorescent Protein ◽  
Fluorescent Protein ◽  
Fluorescence Properties ◽  
Excited State Dynamics ◽  
Green Fluorescent

scholarly journals Ground state isomerization of a model green fluorescent protein chromophore

FEBS Letters ◽  
2003 ◽  
Vol 549 (1-3) ◽  
pp. 35-38 ◽  
Author(s):  
Xiang He ◽  
Alasdair F. Bell ◽  
Peter J. Tonge
Keyword(s):  
Green Fluorescent Protein ◽  
Ground State ◽  
Fluorescent Protein ◽  
Green Fluorescent
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