scholarly journals Click-based amplification: designed to facilitate various target labelling modes with ultralow background amplification

2021 ◽  
Author(s):  
Jinyi Bai ◽  
Fusheng Guo ◽  
Mengyao Li ◽  
Yulong Li ◽  
Xiaoguang Lei
Keyword(s):  
Signal Amplification ◽  
Amplification Method ◽  
Covalent Inhibitor ◽  
Genetic Incorporation

We describe a signal amplification method termed “Click-based amplification” that can be well integrated with various click-labelling modes, including chemical labelling, genetic incorporation and covalent inhibitor probe mediated target labelling.

scholarly journals SARS-CoV-2 RNA Detection with Duplex-Specific Nuclease Signal Amplification

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 197
Author(s):  
Meiqing Liu ◽  
Haoran Li ◽  
Yanwei Jia ◽  
Pui-In Mak ◽  
Rui P. Martins
Keyword(s):  
Signal Amplification ◽  
Incubation Temperature ◽  
Dna Probes ◽  
Detection Sensitivity ◽  
N Gene ◽  
Rna Detection ◽  
Gold Standard Method ◽  
Complementary Method ◽  
Amplification Method ◽  
Virus Rna

The emergence of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a zoonotic pathogen, has led to the outbreak of coronavirus disease 2019 (COVID-19) pandemic and brought serious threats to public health worldwide. The gold standard method for SARS-CoV-2 detection requires both reverse transcription (RT) of the virus RNA to cDNA and then polymerase chain reaction (PCR) for the cDNA amplification, which involves multiple enzymes, multiple reactions and a complicated assay optimization process. Here, we developed a duplex-specific nuclease (DSN)-based signal amplification method for SARS-CoV-2 detection directly from the virus RNA utilizing two specific DNA probes. These specific DNA probes can hybridize to the target RNA at different locations in the nucleocapsid protein gene (N gene) of SARS-CoV-2 to form a DNA/RNA heteroduplex. DSN cleaves the DNA probe to release fluorescence, while leaving the RNA strand intact to be bound to another available probe molecule for further cleavage and fluorescent signal amplification. The optimized DSN amount, incubation temperature and incubation time were investigated in this work. Proof-of-principle SARS-CoV-2 detection was demonstrated with a detection sensitivity of 500 pM virus RNA. This simple, rapid, and direct RNA detection method is expected to provide a complementary method for the detection of viruses mutated at the PCR primer-binding regions for a more precise detection.

Mass Spectrometry Signal Amplification Method for Attomolar Detection of Antigens Using Small-Molecule-Tagged Gold Microparticles

2008 ◽  
Vol 120 (49) ◽  
pp. 9660-9663 ◽  
Author(s):  
Jung Rok Lee ◽  
Juhee Lee ◽  
Sang Kyung Kim ◽  
Kwang Pyo Kim ◽  
Hyung Soon Park ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Small Molecule ◽  
Signal Amplification ◽  
Amplification Method

scholarly journals Signal amplification method for miR-205 assay through combining graphene oxide with duplex-specific nuclease

RSC Advances ◽  
2019 ◽  
Vol 9 (47) ◽  
pp. 27341-27346 ◽  
Author(s):  
Zhaoqi Yang ◽  
Lan Qin ◽  
Dutao Yang ◽  
Weixia Chen ◽  
Yue Qian ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Nasopharyngeal Carcinoma ◽  
Signal Amplification ◽  
Amplification Method ◽  
Radiation Resistant

A signal amplified method for detecting a biomarker of radiation-resistant nasopharyngeal carcinoma using graphene oxide and duplex-specific nuclease was constructed.

A dual signal amplification method for exosome detection based on DNA dendrimer self-assembly

The Analyst ◽  
2019 ◽  
Vol 144 (6) ◽  
pp. 1995-2002 ◽  
Author(s):  
Mei-Ling Gao ◽  
Fang He ◽  
Bin-Cheng Yin ◽  
Bang-Ce Ye
Keyword(s):  
Self Assembly ◽  
Signal Amplification ◽  
Vital Role ◽  
Amplification Method ◽  
Dual Signal Amplification

An increasing number of studies have found that circulating exosomes play a vital role in the occurrence and metastasis of cancer.

Amplification systems of weak interaction biosensors: applications and prospects

Sensor Review ◽  
2015 ◽  
Vol 35 (1) ◽  
pp. 30-42 ◽  
Author(s):  
Xingya Wang ◽  
Guangchang Pang
Keyword(s):  
G Protein ◽  
Weak Interaction ◽  
Signal Amplification ◽  
Signaling Cascade ◽  
G Protein Signaling ◽  
Protein Signaling ◽  
Content Type ◽  
Amplification Method ◽  
Amplification System ◽  
Cascade Amplification

Purpose – This paper aims to provide a detailed review of weak interaction biosensors and several common biosensor methods for magnifying signals, as well as judiciously guide readers through selecting an appropriate detecting system and signal amplification method according to their research and application purpose. Design/methodology/approach – This paper classifies the weak interactions between biomolecules, summarizes the common signal amplification methods used in biosensor design and compares the performance of different kinds of biosensors. It highlights a potential electrochemical signal amplification method: the G protein signaling cascade amplification system. Findings – Developed biosensors which, based on various principles, have their own strengths and weaknesses have met the basic detection requirements for weak interaction between biomolecules: the selectivity, sensitivity and detection limit of biosensors have been consistently improving with the use of new signal amplification methods. However, most of the weak interaction biosensors stop at the research stage; there are only a minority realization of final commercial application. Originality/value – This paper evaluates the status of research and application of weak interaction biosensors systematically. The G protein signaling cascade amplification system proposal offers a new avenue for the research and development of electrochemical biosensors.

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