Deoxyribonucleic Nucleic Acid Anchored on Cell Membranes for Biomedical Applications

2021 ◽  
Author(s):  
Qunye He ◽  
Yanfei Liu ◽  
Ke Li ◽  
Yuwei Wu ◽  
Ting Wang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Biomedical Applications ◽  
Cell Membranes ◽  
Cellular Membrane ◽  
Molecular Tool ◽  
Cellular Behaviors

Engineering cellular membrane with functional molecules provides an attractive strategy to manipulate cellular behaviors and functionalities. Currently, synthetic deoxyribonucleic nucleic acid (DNA) has been emerged as a promising molecular tool...

Penetration and preferential binding of charged nanoparticles to mixed lipid monolayers: interplay of lipid packing and charge density

Soft Matter ◽  
2020 ◽  
Author(s):  
Anurag Chaudhury ◽  
Koushik Debnath ◽  
Wei Bu ◽  
Nikhil R. Jana ◽  
Jaydeep Kumar Basu
Keyword(s):  
Charge Density ◽  
Biomedical Applications ◽  
Cell Membranes ◽  
Lipid Monolayers ◽  
Cytotoxic Effects ◽  
Lipid Packing ◽  
Preferential Binding ◽  
Charged Nanoparticles

Designing of nanoparticles (NPs) for biomedical applications or mitigating their cytotoxic effects require microscopic understanding of their interactions with cell membranes. Such insight is best obtained by studying model biomembranes...

scholarly journals Nucleic acid–based aggregates and their biomedical applications

Aggregate ◽  
2021 ◽  
Vol 2 (1) ◽  
pp. 133-144
Author(s):  
Run Tian ◽  
Zhaoran Wang ◽  
Jianbing Liu ◽  
Qiao Jiang ◽  
Baoquan Ding
Keyword(s):  
Nucleic Acid ◽  
Biomedical Applications

scholarly journals Identification of Novel Subcellular Localization and Trafficking of HIV-1 Nef Variants from Reference Strains G (F1.93.HH8793) and H (BE.93.VI997)

Viruses ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 493
Author(s):  
Logan Van Nynatten ◽  
Aaron Johnson ◽  
Brennan Dirk ◽  
Emily Pawlak ◽  
Rajesh Jacob ◽  
...  
Keyword(s):  
Disease Progression ◽  
Membrane Trafficking ◽  
Protein Function ◽  
Flow Cytometric Analysis ◽  
Cellular Membrane ◽  
Cellular Behaviors ◽  
Cellular Markers ◽  
Golgi Network ◽  
Reference Strains ◽  
Hiv 1

The human immunodeficiency virus type 1 (HIV-1) accessory protein Nef, plays an essential role in disease progression and pathogenesis via hijacking the host cellular membrane-trafficking machinery. Interestingly, HIV-1 group-M subtypes display differences in the rate of disease progression. However, few reports investigated how the cellular behaviors and activities of Nef isolates from reference strains may differ between HIV-1 group-M subtypes. Here, we characterize how differing cellular distributions of Nef proteins across group-M subtypes may impact protein function using immunofluorescence microscopy and flow cytometric analysis. We demonstrate that Nef variants isolated from HIV-1 group-M subtypes display differences in expression, with low expressing Nef proteins from reference strains of subtypes G (F1.93.HH8793) and H (BE.93.VI997) also displaying decreased functionality. Additionally, we demonstrate variations in the subcellular distribution and localization of these Nef proteins. Nef from subtype G (F1.93.HH8793) and H (BE.93.VI997) reference strains also failed to colocalize with the trans-Golgi network, and were not differentially localized to cellular markers of multivesicular bodies or lysosomes. Strikingly, our results demonstrate that HIV-1 Nef proteins from reference strains G (F1.93.HH8793) and H (BE.93.VI997) highly colocalize with labeled mitochondrial compartments.

Nucleic Acid Guided Molecular Tool for In-Vivo Theranostic Applications

Aptamers ◽  
2019 ◽  
pp. 101-122
Author(s):  
Shahnawaz Ahmad Baba ◽  
Ruchi Mutreja ◽  
Arun Beniwal ◽  
Shubham Jain ◽  
Ekta Yadav ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Molecular Tool ◽  
Theranostic Applications

Biomedical Applications of Quantum Dots, Nucleic Acid-Based Aptamers, and Nanostructures in Biosensors

2015 ◽  
Vol 43 (4) ◽  
pp. 277-296 ◽  
Author(s):  
Xenia Meshik ◽  
Sidra Farid ◽  
Min Choi ◽  
Yi Lan ◽  
Souvik Mukherjee ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Nucleic Acid ◽  
Biomedical Applications

scholarly journals Deficiency of stearoyl-CoA desaturase-1 aggravates colitogenic potential of adoptively transferred effector T cells

2016 ◽  
Vol 311 (4) ◽  
pp. G713-G723 ◽  
Author(s):  
Beng San Yeoh ◽  
Piu Saha ◽  
Vishal Singh ◽  
Xia Xiao ◽  
Yun Ying ◽  
...  
Keyword(s):  
T Cells ◽  
Lipid Bilayers ◽  
Cell Membranes ◽  
Intestinal Inflammation ◽  
De Novo ◽  
Saturated Fatty Acids ◽  
Cellular Membrane ◽  
Major Fatty Acid ◽  
Effector T Cells ◽  
Stearoyl Coa Desaturase

Stearoyl-CoA desaturase-1 (SCD1) is a lipogenic enzyme involved in the de novo biosynthesis of oleate (C18:1, n9), a major fatty acid in the phospholipids of lipid bilayers of cell membranes. Accordingly, Scd1KO mice display substantially reduced oleate in cell membranes. An altered SCD1 level was observed during intestinal inflammation; however, its role in modulating inflammatory bowel disease remains elusive. Herein, we investigated the colitogenic capacity of Scd1KO effector T cells by employing the adoptive T-cell transfer colitis model. Splenic effector T cells (CD4+CD25−) from age- and sex-matched wild-type (WT) and Scd1KO mice were isolated by FACS and intraperitoneally administered to Rag1KO mice, which were monitored for the development of colitis. At day 60 postcell transfer, Rag1KO mice that received Scd1KO CD4+CD25− T cells displayed accelerated and exacerbated colitis than mice receiving WT CD4+CD25− T cells. Intriguingly, Scd1KO CD4+CD25− T cells display augmented inflammatory cytokine profile and cellular membrane fluidity with a concomitant increase in proinflammatory saturated fatty acids, which we postulate to potentially underlie their augmented colitogenic potential.

scholarly journals Tunability of Biodegradable Poly(amine-co-ester) Polymers for Customized Nucleic Acid Delivery and Other Biomedical Applications

2018 ◽  
Vol 19 (9) ◽  
pp. 3861-3873 ◽  
Author(s):  
Amy C. Kauffman ◽  
Alexandra S. Piotrowski-Daspit ◽  
Kay H. Nakazawa ◽  
Yuhang Jiang ◽  
Amit Datye ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Biomedical Applications ◽  
Nucleic Acid Delivery ◽  
Biodegradable Poly

scholarly journals A survey of advancements in nucleic acid-based logic gates and computing for applications in biotechnology and biomedicine

2015 ◽  
Vol 51 (18) ◽  
pp. 3723-3734 ◽  
Author(s):  
Cuichen Wu ◽  
Shuo Wan ◽  
Weijia Hou ◽  
Liqin Zhang ◽  
Jiehua Xu ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Biomedical Applications ◽  
Logic Gates ◽  
Logic Systems

Nucleic acid based logic systems have been rationally designed and functionalized to better serve bioanalytical and biomedical applications.

Towards biomedical applications for nucleic acid nanodevices

Nanomedicine ◽  
2007 ◽  
Vol 2 (6) ◽  
pp. 817-830 ◽  
Author(s):  
Friedrich C Simmel
Keyword(s):  
Nucleic Acid ◽  
Biomedical Applications
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