A Fluorescent Color Card for Point-of-care Testing (POCT) and Its Application in Simultaneous Detection

The Analyst ◽  
2021 ◽  
Author(s):  
Fan Lu ◽  
Sisi Yang ◽  
Yu Ning ◽  
Fubing Wang ◽  
Xinghu Ji ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Point Of Care ◽  
Simultaneous Detection ◽  
Cerebrovascular Diseases ◽  
Point Of Care Testing

Diabetes mellitus has been paid much attention because it complicates the liver, kidney, eye, heart, and cerebrovascular diseases. Thus, it exhibits a positive significance to develop a rapid and efficient...

scholarly journals Wave-shaped microfluidic chip assisted point-of-care testing for accurate and rapid diagnosis of infections

2021 ◽  
Author(s):  
Binfeng Yin ◽  
Xinhua Wan ◽  
Mingzhu Yang ◽  
Changcheng Qian ◽  
A S M Muhtasim Fuad Sohan
Keyword(s):  
Microfluidic Chip ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Simultaneous Detection ◽  
Accurate Diagnosis ◽  
Point Of Care Testing ◽  
Promising Alternative ◽  
Reactive Protein ◽  
Linear Relationships ◽  
Multiple Biomarkers

Abstract Background: Simultaneous and timely detection of C-reactive protein (CRP), procalcitonin (PCT), and interleukin-6 (IL-6) provides effective information for the accurate diagnosis of infections. Early diagnosis and classification of infections increase the cure rate while decreasing complications, which is significant for severe infections, especially for war surgery. However, traditional methods rely on laborious operations and bulky devices. On the other hand, point-of-care (POC) methods suffer from limited robustness and accuracy. Therefore, it is of urgent demand to develop POC devices for rapid and accurate diagnosis of infections to fulfill on-site militarized requirements.Methods: We developed a wave-shaped microfluidic chip (WMC) assisted multiplexed detection platform (WMC-MDP). WMC-MDP reduces detection time and improves repeatability through premixing of the samples and reaction of the reagents. We further combined the detection platform with the streptavidin-biotin (SA-B) amplified system to enhance the sensitivity while using chemiluminescence (CL) intensity as signal readout. We realized simultaneous detection of CRP, PCT, and IL-6 on the detection platform and evaluated the sensitivity, linear range, selectivity, and repeatability. Finally, we finished detecting 15 samples from volunteers and compared the results with commercial ELISA kits.Results: Detection of CRP, PCT, and IL-6 exhibited good linear relationships between CL intensities and concentrations in the range of 1.25-40 μg/mL, 0.4-12.8 ng/mL, and 50-1600 pg/mL. The limit of detection (LOD) of CRP, PCT, and IL-6 were 0.54 μg/mL, 0.11 ng/mL, and 16.25 pg/mL, respectively. WMC-MDP is capable of good adequate selectivity and repeatability. The whole detection procedure takes only 22 minutes that meets the requirements of a POC device. Results of 15 samples from volunteers were consistent with the results detected by commercial ELISA kits.Conclusion: WMC-MDP allows simultaneous, rapid, and sensitive detection of CRP, PCT, and IL-6 with satisfactory selectivity and repeatability, requiring minimal manipulation. However, WMC-MDP takes advantage of being a microfluidic device showing the coefficients of variation less than 10% enabling WMC-MDP to be a type of POCT. Therefore, WMC-MDP provides a promising alternative to point-of-care testing (POCT) of multiple biomarkers. We believe the practical application of WMC-MDP in militarized fields will revolutionize infection diagnosis for soldiers.

scholarly journals Three-Dimensional Paper-Based Microfluidic Analysis Device for Simultaneous Detection of Multiple Biomarkers with a Smartphone

Biosensors ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 187
Author(s):  
Seung Ho Baek ◽  
Chanyong Park ◽  
Jaehyung Jeon ◽  
Sungsu Park
Keyword(s):  
Point Of Care ◽  
Simultaneous Detection ◽  
Three Dimensional ◽  
Cost Effective ◽  
Smartphone Application ◽  
Accurate Diagnosis ◽  
Point Of Care Testing ◽  
Digital Light Processing ◽  
Multiple Biomarkers ◽  
Microfluidic Analysis

Paper-based microfluidic analysis devices (μPADs) have attracted attention as a cost-effective platform for point-of-care testing (POCT), food safety, and environmental monitoring. Recently, three-dimensional (3D)-μPADs have been developed to improve the performance of μPADs. For accurate diagnosis of diseases, however, 3D-μPADs need to be developed to simultaneously detect multiple biomarkers. Here, we report a 3D-μPADs platform for the detection of multiple biomarkers that can be analyzed and diagnosed with a smartphone. The 3D-μPADs were fabricated using a 3D digital light processing printer and consisted of a sample reservoir (300 µL) connected to 24 detection zones (of 4 mm in diameter) through eight microchannels (of 2 mm in width). With the smartphone application, eight different biomarkers related to various diseases were detectable in concentrations ranging from normal to abnormal conditions: glucose (0–20 mmol/L), cholesterol (0–10 mmol/L), albumin (0–7 g/dL), alkaline phosphatase (0–800 U/L), creatinine (0–500 µmol/L), aspartate aminotransferase (0–800 U/L), alanine aminotransferase (0–1000 U/L), and urea nitrogen (0–7.2 mmol/L). These results suggest that 3D-µPADs can be used as a POCT platform for simultaneous detection of multiple biomarkers.

Point-of-Care Testing device for analysis of Diabetes Mellitus

2013 ◽  
Author(s):  
J. A. Oliveira ◽  
G. Minas ◽  
M. Correia-Neves ◽  
J. Mariz ◽  
C. Capela ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Point Of Care ◽  
Testing Device ◽  
Point Of Care Testing

scholarly journals Three-Dimensional Paper-Based Microfluidic Analysis Device for Simultaneous Detection of Multiple Biomarkers With a Smartphone

2020 ◽  
Author(s):  
Seung Ho Baek ◽  
Chanyong Park ◽  
Jaehyung Jeon ◽  
Sungsu Park
Keyword(s):  
Point Of Care ◽  
Simultaneous Detection ◽  
Three Dimensional ◽  
Cost Effective ◽  
Smartphone Application ◽  
Accurate Diagnosis ◽  
Point Of Care Testing ◽  
Digital Light Processing ◽  
Multiple Biomarkers ◽  
Microfluidic Analysis

Paper-based microfluidic analysis devices (μPADs) have attracted attention as a cost-effective platform for point-of-care testing (POCT), food safety, and environmental monitoring. Recently, three-dimensional (3D)-μPADs have been developed to improve the performance of μPADs. For accurate diagnosis of diseases, however, 3D-μPADs need to be developed to simultaneously detect multiple biomarkers. Here, we report a 3D-μPADs platform for the detection of multiple biomarkers that can be analyzed and diagnosed with a smartphone. The 3D-μPADs were fabricated using a 3D digital light processing printer and consisted of a sample reservoir (300 µL) connected to 24 detection zones (of 4 mm in diameter) through 8 microchannels (of 2 mm in width). With the smartphone application, eight different biomarkers related to various diseases were detectable in concentrations ranging from normal to abnormal conditions: glucose (0–20 mmol/L), cholesterol (0–10 mmol/L), albumin (0–7 g/dL), alkaline phosphatase (0–800 U/L), creatinine (0–500 µmol/L), aspartate aminotransferase (0–800 U/L), alanine aminotransferase (0–1000 U/L), and urea nitrogen (0–7.2 mmol/L). These results suggest that 3D-µPADs can be used as a POCT platform for simultaneous detection of multiple biomarkers.

scholarly journals Point-of-Care Testing Device for Diabetes Mellitus and Renal Function Analysis of Biological Fluids

2012 ◽  
Vol 47 ◽  
pp. 710-713 ◽  
Author(s):  
J.A. Oliveira ◽  
José Mariz ◽  
Carlos Capela ◽  
M. Correia-Neves ◽  
G. Minas
Keyword(s):  
Diabetes Mellitus ◽  
Renal Function ◽  
Function Analysis ◽  
Biological Fluids ◽  
Point Of Care ◽  
Testing Device ◽  
Point Of Care Testing
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