scholarly journals Three-Dimensional Paper-Based Microfluidic Analysis Device for Simultaneous Detection of Multiple Biomarkers with a Smartphone

Biosensors ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 187
Author(s):  
Seung Ho Baek ◽  
Chanyong Park ◽  
Jaehyung Jeon ◽  
Sungsu Park
Keyword(s):  
Point Of Care ◽  
Simultaneous Detection ◽  
Three Dimensional ◽  
Cost Effective ◽  
Smartphone Application ◽  
Accurate Diagnosis ◽  
Point Of Care Testing ◽  
Digital Light Processing ◽  
Multiple Biomarkers ◽  
Microfluidic Analysis

Paper-based microfluidic analysis devices (μPADs) have attracted attention as a cost-effective platform for point-of-care testing (POCT), food safety, and environmental monitoring. Recently, three-dimensional (3D)-μPADs have been developed to improve the performance of μPADs. For accurate diagnosis of diseases, however, 3D-μPADs need to be developed to simultaneously detect multiple biomarkers. Here, we report a 3D-μPADs platform for the detection of multiple biomarkers that can be analyzed and diagnosed with a smartphone. The 3D-μPADs were fabricated using a 3D digital light processing printer and consisted of a sample reservoir (300 µL) connected to 24 detection zones (of 4 mm in diameter) through eight microchannels (of 2 mm in width). With the smartphone application, eight different biomarkers related to various diseases were detectable in concentrations ranging from normal to abnormal conditions: glucose (0–20 mmol/L), cholesterol (0–10 mmol/L), albumin (0–7 g/dL), alkaline phosphatase (0–800 U/L), creatinine (0–500 µmol/L), aspartate aminotransferase (0–800 U/L), alanine aminotransferase (0–1000 U/L), and urea nitrogen (0–7.2 mmol/L). These results suggest that 3D-µPADs can be used as a POCT platform for simultaneous detection of multiple biomarkers.

scholarly journals Three-Dimensional Paper-Based Microfluidic Analysis Device for Simultaneous Detection of Multiple Biomarkers With a Smartphone

2020 ◽  
Author(s):  
Seung Ho Baek ◽  
Chanyong Park ◽  
Jaehyung Jeon ◽  
Sungsu Park
Keyword(s):  
Point Of Care ◽  
Simultaneous Detection ◽  
Three Dimensional ◽  
Cost Effective ◽  
Smartphone Application ◽  
Accurate Diagnosis ◽  
Point Of Care Testing ◽  
Digital Light Processing ◽  
Multiple Biomarkers ◽  
Microfluidic Analysis

Paper-based microfluidic analysis devices (μPADs) have attracted attention as a cost-effective platform for point-of-care testing (POCT), food safety, and environmental monitoring. Recently, three-dimensional (3D)-μPADs have been developed to improve the performance of μPADs. For accurate diagnosis of diseases, however, 3D-μPADs need to be developed to simultaneously detect multiple biomarkers. Here, we report a 3D-μPADs platform for the detection of multiple biomarkers that can be analyzed and diagnosed with a smartphone. The 3D-μPADs were fabricated using a 3D digital light processing printer and consisted of a sample reservoir (300 µL) connected to 24 detection zones (of 4 mm in diameter) through 8 microchannels (of 2 mm in width). With the smartphone application, eight different biomarkers related to various diseases were detectable in concentrations ranging from normal to abnormal conditions: glucose (0–20 mmol/L), cholesterol (0–10 mmol/L), albumin (0–7 g/dL), alkaline phosphatase (0–800 U/L), creatinine (0–500 µmol/L), aspartate aminotransferase (0–800 U/L), alanine aminotransferase (0–1000 U/L), and urea nitrogen (0–7.2 mmol/L). These results suggest that 3D-µPADs can be used as a POCT platform for simultaneous detection of multiple biomarkers.

scholarly journals Wave-shaped microfluidic chip assisted point-of-care testing for accurate and rapid diagnosis of infections

2021 ◽  
Author(s):  
Binfeng Yin ◽  
Xinhua Wan ◽  
Mingzhu Yang ◽  
Changcheng Qian ◽  
A S M Muhtasim Fuad Sohan
Keyword(s):  
Microfluidic Chip ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Simultaneous Detection ◽  
Accurate Diagnosis ◽  
Point Of Care Testing ◽  
Promising Alternative ◽  
Reactive Protein ◽  
Linear Relationships ◽  
Multiple Biomarkers

Abstract Background: Simultaneous and timely detection of C-reactive protein (CRP), procalcitonin (PCT), and interleukin-6 (IL-6) provides effective information for the accurate diagnosis of infections. Early diagnosis and classification of infections increase the cure rate while decreasing complications, which is significant for severe infections, especially for war surgery. However, traditional methods rely on laborious operations and bulky devices. On the other hand, point-of-care (POC) methods suffer from limited robustness and accuracy. Therefore, it is of urgent demand to develop POC devices for rapid and accurate diagnosis of infections to fulfill on-site militarized requirements.Methods: We developed a wave-shaped microfluidic chip (WMC) assisted multiplexed detection platform (WMC-MDP). WMC-MDP reduces detection time and improves repeatability through premixing of the samples and reaction of the reagents. We further combined the detection platform with the streptavidin-biotin (SA-B) amplified system to enhance the sensitivity while using chemiluminescence (CL) intensity as signal readout. We realized simultaneous detection of CRP, PCT, and IL-6 on the detection platform and evaluated the sensitivity, linear range, selectivity, and repeatability. Finally, we finished detecting 15 samples from volunteers and compared the results with commercial ELISA kits.Results: Detection of CRP, PCT, and IL-6 exhibited good linear relationships between CL intensities and concentrations in the range of 1.25-40 μg/mL, 0.4-12.8 ng/mL, and 50-1600 pg/mL. The limit of detection (LOD) of CRP, PCT, and IL-6 were 0.54 μg/mL, 0.11 ng/mL, and 16.25 pg/mL, respectively. WMC-MDP is capable of good adequate selectivity and repeatability. The whole detection procedure takes only 22 minutes that meets the requirements of a POC device. Results of 15 samples from volunteers were consistent with the results detected by commercial ELISA kits.Conclusion: WMC-MDP allows simultaneous, rapid, and sensitive detection of CRP, PCT, and IL-6 with satisfactory selectivity and repeatability, requiring minimal manipulation. However, WMC-MDP takes advantage of being a microfluidic device showing the coefficients of variation less than 10% enabling WMC-MDP to be a type of POCT. Therefore, WMC-MDP provides a promising alternative to point-of-care testing (POCT) of multiple biomarkers. We believe the practical application of WMC-MDP in militarized fields will revolutionize infection diagnosis for soldiers.

Paper-based point-of-care testing for cost-effective diagnosis of acute dengue infections

2016 ◽  
Vol 82 ◽  
pp. S51
Author(s):  
F. Bedin ◽  
E. Voilin ◽  
L. Boulet ◽  
G. Theillet ◽  
A. Perrin ◽  
...  
Keyword(s):  
Point Of Care ◽  
Cost Effective ◽  
Point Of Care Testing ◽  
Effective Diagnosis

scholarly journals Textile sensors platform for the selective and simultaneous detection of chloride ion and pH in sweat

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Luca Possanzini ◽  
Francesco Decataldo ◽  
Federica Mariani ◽  
Isacco Gualandi ◽  
Marta Tessarolo ◽  
...  
Keyword(s):  
Point Of Care ◽  
Wearable Sensors ◽  
Simultaneous Detection ◽  
Chloride Ion ◽  
Cost Effective ◽  
Chloride Ions ◽  
Buffer Solution ◽  
Textile Sensors ◽  
New Vision ◽  
Poly Styrene Sulfonate

Abstract The development of wearable sensors, in particular fully-textile ones, is one of the most interesting open challenges in bioelectronics. Several and significant steps forward have been taken in the last decade in order to achieve a compact, lightweight, cost-effective, and easy to wear platform for healthcare and sport activities real-time monitoring. We have developed a fully textile, multi-thread biosensing platform that can detect different bioanalytes simultaneously without interference, and, as an example, we propose it for testing chloride ions (Cl−) concentration and pH level. The textile sensors are simple threads, based on natural and synthetic fibers, coated with the conducting polymer poly(3,4-ethylenedioxythiophene):poly(styrene-sulfonate) (PEDOT:PSS) and properly functionalized with either a nano-composite material or a chemical sensitive dye to obtain Cl− and pH selective sensing functionality, respectively. The single-thread sensors show excellent sensitivity, reproducibility, selectivity, long term stability and the ability to work with small volumes of solution. The performance of the developed textile devices is demonstrated both in buffer solution and in artificial human perspiration to perform on-demand and point-of-care epidermal fluids analysis. The possibility to easily knit or sew the thread sensors into fabrics opens up a new vision for a textile wearable multi-sensing platform achievable in the near future.

scholarly journals Cost-Effective Flexible CSRR-Based Sensor for Noninvasive Measurement of Permittivity of Biomaterials

2021 ◽  
Vol 10 (1) ◽  
pp. 26
Author(s):  
Faezeh Shanehsazzadeh ◽  
Nafise Azizi ◽  
Hosna Kazerooni Haghighat ◽  
Fatemeh Mashayekhi ◽  
Mehdi Fardmanesh
Keyword(s):  
Ring Resonator ◽  
Point Of Care ◽  
Cost Effective ◽  
Split Ring Resonator ◽  
Point Of Care Testing ◽  
Split Ring ◽  
Tissue Samples ◽  
Complementary Split Ring Resonator ◽  
Microwave Sensor ◽  
Wide Range

A novel, cost-effective, flexible microwave sensor is proposed to facilitate point-of-care testing (POCT) methods for medical diagnosis. The sensor is based on the complementary split-ring resonator (CSRR) to accurately measure the permittivity of biomaterials over a wide range of frequencies. This ability can be used to characterize various materials under test (MUT) such as blood, saliva, tissue samples, etc. The flexibility of the proposed sensor means that it can be used when the accessibility of the sample has technical difficulties, such as on curved surfaces. Firstly, the optimized structure and coupling to the readout transmission line are evaluated using finite element method (FEM) simulations. Then, the prototype of the optimized structure is fabricated on a thin polydimethylsiloxane (PDMS) substrate as a biocompatible economical polymer, and aluminium is carefully chosen for the fabrication of CSRR and readout parts. The proposed flexible sensor is tested and compared to conventional rigid CSRR sensors. The proposed structure withstood the different bending positions well, and also showed an improvement in the results for curved MUT.

scholarly journals Paper-based point-of-care testing for cost-effective diagnosis of acute flavivirus infections

2017 ◽  
Vol 89 (9) ◽  
pp. 1520-1527 ◽  
Author(s):  
Frederic Bedin ◽  
Laurent Boulet ◽  
Elodie Voilin ◽  
Gerald Theillet ◽  
Agnes Rubens ◽  
...  
Keyword(s):  
Point Of Care ◽  
Cost Effective ◽  
Point Of Care Testing ◽  
Effective Diagnosis

Point-of-care testing INR: an overview

2017 ◽  
Vol 55 (6) ◽  
pp. 800-805 ◽  
Author(s):  
Doris Barcellona ◽  
Lara Fenu ◽  
Francesco Marongiu
Keyword(s):  
Point Of Care ◽  
Cost Effective ◽  
International Normalized Ratio ◽  
Point Of Care Testing ◽  
Mechanical Valves ◽  
Self Testing ◽  
Periodic Monitoring ◽  
Monitoring Test ◽  
Pros And Cons

Abstract Oral anticoagulant therapies with the anti-vitamin K drugs (AVK), warfarin, acenocoumarol and phenprocoumon, are employed in primary and secondary anti-thrombotic prophylaxis in patients with venous thromboembolism, atrial fibrillation and cardiac mechanical valves. However, a monitoring test such as the International Normalized Ratio (INR) is required. The periodic monitoring of this therapy entails discomfort for the patients. Telemedicine and telecare can provide significant aid in the management of this therapy allowing patients to perform the test at home or anywhere else with a portable device, i.e. point-of-care testing (POCT), and to send the result to a thrombosis (TC) via web. Patients can receive dose adjustment sent back by the TC. The effectiveness of this type of management is equal or superior to the traditional AVK monitoring in terms of hemorrhagic and thrombotic events. Analysis of the costs with a horizon of 10 years reveals that both self-testing and self-management are cost-effective. The aim of this overview is to describe the pros and cons of the use of POCT as an alternative in the monitoring of AVK. In particular, description of the POCT, decentralization, quality of the therapy, safety and costs will be examined.

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