Dispersion-Enhancing surface treatment of AuNP for Reduced Probe loading and detection limit using t-SPR detection

The Analyst ◽  
2021 ◽  
Author(s):  
Wing Kiu Yeung ◽  
Shu-Cheng Lo ◽  
Sheng-Hann Wang ◽  
Pei-Kuen Wei ◽  
Ji-Yen Cheng
Keyword(s):  
Surface Plasmon Resonance ◽  
Detection Limit ◽  
Surface Treatment ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Diagnostic System ◽  
Spectral Imaging ◽  
Microfluidic Biosensor

COVID-19 has demonstrated that a highly specific and rapid diagnostic system is a necessity. Spectral imaging-based surface plasmon resonance (SPRi) platform with integrated microfluidic biosensor to detect oligonucleotide sequences has...

scholarly journals Femtomolar and selective dopamine detection by a gold nanoparticle enhanced surface plasmon resonance aptasensor

2018 ◽  
Author(s):  
Yong Cao ◽  
Mark T. McDermott
Keyword(s):  
Surface Plasmon Resonance ◽  
Detection Limit ◽  
Gold Nanoparticle ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Cdna Probe ◽  
Dna Aptamer ◽  
Dopamine Detection ◽  
Calibration Curves ◽  
20 Nm

ABSTRACTUltrasensitive and selective detection and quantification of dopamine (DA) plays a key role in monitoring neurodegenerative diseases. However, the detection limit reported for DA detection is typically in the lower nM range. Pushing the detection limit to pM or lower for this particular target to cover the physiological levels (< 130 pM) is significant. Herein, DA DNA aptamer (DAAPT) gold nanoparticle (AuNP) conjugate is utilized to enhance the surface plasmon resonance (SPR) signal, which enables to detect and quantify DA in the femtomolar (200 fM) to picomolar range. To the best of our knowledge, this is the lowest detection limit achieved for SPR sensing of dopamine. The as-prepared 10 nm DAAPT-AuNP conjugate demonstrates strong binding affinity (Kd = 3.1 ± 1.4 nM) to the complementary DNA (cDNA) probe on gold chip. The cDNA probe is immobilized to the chip via polydopamine surface chemistry, which allows the Michael addition of any primary amine-terminated biomolecules. By adjusting the concentration of the DAAPT-AuNP conjugate, two calibration curves are generated with dynamic ranges from 100 µM to 2 mM, and from 200 fM to 20 nM, respectively. Both calibration curves have negative slopes, showing good agreement to a dose-response curve in an enzyme inhibition assay. In addition, the sensing strategy is evaluated to be specific for DA detection using a series of DA analogs and other metabolites as potential interferences.

A Hydride Generation-Nanosilver Surface Plasmon Resonance Absorption Spectral Method for the Determination of Trace Se(IV)

2013 ◽  
Vol 749 ◽  
pp. 491-494
Author(s):  
Ai Hui Liang ◽  
Qing Ye Liu ◽  
Gui Qing Wen ◽  
Ting Sheng Li ◽  
Zhi Liang Jiang
Keyword(s):  
Surface Plasmon Resonance ◽  
Detection Limit ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Water Samples ◽  
Hydride Generation ◽  
Resonance Absorption ◽  
Surface Plasmon Resonance Absorption ◽  
Hcl Solution

In HCl solution, the Se (IV) was reduced to SeH2 by NaBH4, and absorbed by solution of ethanol-AgNO3. The Ag+ was reduced to nanosilver that exhibited surface plasmon resonance absorption (SPR) peaks at 292 nm and 420 nm. Under the selected conditions, the value at 292 nm was linear to the concentration of Se (IV) in the range of 0.08-2.0 μg/mL, a detection limit of 0.04 μg/mL. The proposed method was applied to detect Se (IV) in water samples, with satisfactory results.

Highly Sensitive Surface Plasmon Resonance Sensor on Nanoscale Bioactive Surfaces for Specific Detection of Tri-Nitro Toluene

2006 ◽  
Vol 951 ◽  
Author(s):  
Praveen Singh ◽  
Takeshi Onodera ◽  
Kiyoshi Matsumoto ◽  
Norio Miura ◽  
Kiyoshi Toko
Keyword(s):  
Surface Plasmon Resonance ◽  
Detection Limit ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Self Assembly ◽  
Competitive Inhibition ◽  
Layer By Layer ◽  
Self Assembled Monolayer ◽  
Chip Surface ◽  
Resonance Sensor

ABSTRACTA nano-scale biosensor chip surface was fabricated using dinitro-phenylated key hole limpet (DNP-KLH) protein conjugate as ligand supported by underlying 11-amino 1-undecanethiol hydrochloride(AUT) self assembled monolayer (SAM) and bis sulfosuccinimidyl suberate(BS3) as crosslinker. Bioactive thin films were fabricated over gold chip via layer-by-layer self assembly methods. Biomolecular interaction between substrate specific anti-TNT antibody and DNP-KLH conjugate surface was monitored through surface plasmon resonance based optical sensor. The quantitation of tri-nitro toluene(TNT) on this bioactive surface was done using the solution based competitive inhibition assay. The DNP-KLH surface biosensor has shown a detection limit of 0.14 ng/ml(140 ppt) for TNT molecule. The detection limit of surface plasmon resonance(SPR) biosensor was further enhanced by using goat anti mouse antibody to the 0.002 ng/ml for TNT analyte. This TNT specific biosensor holds the promise to be one of most sensitive sensor surface under indirect competitive assay format. A short injection (12 sec) of 10 mM Glycine-HCl solution was found adequate for regeneration of DNP-KLH surface for repeated use.

scholarly journals Emergent Biosensing Technologies Based on Fluorescence Spectroscopy and Surface Plasmon Resonance

Sensors ◽  
2021 ◽  
Vol 21 (3) ◽  
pp. 906
Author(s):  
Alessandra Camarca ◽  
Antonio Varriale ◽  
Alessandro Capo ◽  
Angela Pennacchio ◽  
Alessia Calabrese ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Detection Limit ◽  
Fluorescence Spectroscopy ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Food Environment ◽  
Daily Life ◽  
Commercial Potential ◽  
Scientific Disciplines ◽  
Wide Range

The purpose of this work is to provide an exhaustive overview of the emerging biosensor technologies for the detection of analytes of interest for food, environment, security, and health. Over the years, biosensors have acquired increasing importance in a wide range of applications due to synergistic studies of various scientific disciplines, determining their great commercial potential and revealing how nanotechnology and biotechnology can be strictly connected. In the present scenario, biosensors have increased their detection limit and sensitivity unthinkable until a few years ago. The most widely used biosensors are optical-based devices such as surface plasmon resonance (SPR)-based biosensors and fluorescence-based biosensors. Here, we will review them by highlighting how the progress in their design and development could impact our daily life.

scholarly journals Localized Surface Plasmon Resonance Decorated with Carbon Quantum Dots and Triangular Ag Nanoparticles for Chlorophyll Detection

Nanomaterials ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 35
Author(s):  
Nur Afifah Ahmad Nazri ◽  
Nur Hidayah Azeman ◽  
Mohd Hafiz Abu Bakar ◽  
Nadhratun Naiim Mobarak ◽  
Yunhan Luo ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Surface Plasmon Resonance ◽  
Detection Limit ◽  
Functional Groups ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Linear Response ◽  
Localized Surface Plasmon Resonance ◽  
Carbon Quantum Dots ◽  
Localized Surface Plasmon

This paper demonstrates carbon quantum dots (CQDs) with triangular silver nanoparticles (AgNPs) as the sensing materials of localized surface plasmon resonance (LSPR) sensors for chlorophyll detection. The CQDs and AgNPs were prepared by a one-step hydrothermal process and a direct chemical reduction process, respectively. FTIR analysis shows that a CQD consists of NH2, OH, and COOH functional groups. The appearance of C=O and NH2 at 399.5 eV and 529.6 eV in XPS analysis indicates that functional groups are available for adsorption sites for chlorophyll interaction. A AgNP–CQD composite was coated on the glass slide surface using (3-aminopropyl) triethoxysilane (APTES) as a coupling agent and acted as the active sensing layer for chlorophyll detection. In LSPR sensing, the linear response detection for AgNP–CQD demonstrates R2 = 0.9581 and a sensitivity of 0.80 nm ppm−1, with a detection limit of 4.71 ppm ranging from 0.2 to 10.0 ppm. Meanwhile, a AgNP shows a linear response of R2 = 0.1541 and a sensitivity of 0.25 nm ppm−1, with the detection limit of 52.76 ppm upon exposure to chlorophyll. Based on these results, the AgNP–CQD composite shows a better linearity response and a higher sensitivity than bare AgNPs when exposed to chlorophyll, highlighting the potential of AgNP–CQD as a sensing material in this study.

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